| Project/Area Number |
24790421
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Kyoto Prefectural University |
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Principal Investigator |
OKA Mayuko 京都府立大学, 生命環境科学研究科(系), 准教授 (40347498)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | マクロファージ / 結核 / HIF-1alpha / 糖代謝 / 低酸素 |
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| Research Abstract |
For intracellular replication of Mycobacterium tuberculosis, glucose is considered one of dominant carbon sources but it is also important for energy production and maintenance cellular functions of the macrophages. However it is unknown how M. tuberculosis infection impacts on the glucose metabolism of the macrophages and in turn, how it affects on the virulence of this pathogen. Hypoxia-inducible factor-1alpha (HIF-1) is one of master regulators of glucose metabolism. In this study we examined whether that HIF-1 has some affects on intracellular growth of M. tuberculosis.
These results shows that HIF-1 protein down-regulates cellular concentration of pyruvate in the intracellular bacterial pathogen-infected macrophages via enhancing LDH-A level, and thus attenuates intracellular replication of M. tuberculosis. Our data demonstrate that regulation of glucose metabolism is critical basic mechanism in controlling intracellular pathogen.
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