Autoantibody production during gamma-herpesvirus infection

• Project/Area Number: 24790440
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: Osaka University
• Principal Investigator: SAKAKIBARA Shuhei 大阪大学, 微生物病研究所, 助教 (10618838)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 自己反応性免疫応答 / 自己抗体産生 / ウイルス / ウイルス感染 / polyreactive抗体

Research Abstract

The purpose of this study is to unveil the underlying mechanisms how autoreactive B cells emerge during viral infection. Murine gamma-herpesvirus 68 (MHV68) reportedly induced serum IgG autoantibody. Employing this model, we analyzed the reactivities of the germinal center B cells by singlecell based clonining method. It was revealed that the splenic IgG-positive germinal center B cells from MHV68-infected mice exhibited auto- and polyreactivity, which is defined as an ability to recognize multiple antigens.

Research Products

• [Journal Article] ウイルス感染による自己抗体先生誘導 (2014)
  • Author(s): 榊原修平、菊谷仁
  • Journal Title: 臨床免疫・アレルギー科 Volume: vol.61, No.1
• [Journal Article] ウイルス感染による自己抗体産生誘導 (2014)
  • Author(s): 榊原 修平･ 菊谷 仁
  • Journal Title: 臨床免疫･アレルギー科 Volume: 61 Pages: 74-80
  • NAID: 40019955503
• [Journal Article] A20/TNFAIP3 inhibits NF-κB activation induced by the Kaposi's sarcoma-associated herpesvirus vFLIP oncoprotein (2013)
  • Author(s): Sakakibara, S., Espigol-Frigole G., Gasperini P., Uldrick T.S., Yarchoan R., and Tosato G.
  • Journal Title: Oncogene Volume: 32 Pages: 1223-1232
  • Peer Reviewed
• [Presentation] Generation of polyreactive B cells during murine herpesvirus infection (2013)
  • Author(s): Sakakibara, S., T. Yasui, T. Minamitani, and H. Kikutani
  • Organizer: 第42回日本免疫学会
  • Place of Presentation: 千葉市(口頭)
• [Presentation] Generation of autoreactive and poly-reactive antibody producing cells during gamma-herpevirus infection (2013)
  • Author(s): Sakakibara, S., T. Yasui, T. Minamitani, H. Jinzai, and H. Kikutani
  • Organizer: The 15 th International Congress of Immunology
  • Place of Presentation: イタリアミラノ(ポスター)
• [Presentation] Generation and selection of virus- and self-reactive B cells during herpesvirus infection (2013)
  • Author(s): Sakakibara, S., T. Yasui, T. Minamitani, and H. Kikutani
  • Organizer: 第41回日本免疫学会
  • Place of Presentation: 神戸市(口頭)
• [Presentation] Generation of self-reactive and poly-reactive antibody producing cells during g-herpesvirus infection (2013)
  • Author(s): ○Shuhei Sakakibara, Teruhito Yasui, Takeharu Minamitani, Hideyuki Jinzai and Hitoshi Kikutani
  • Organizer: The 15 th International Congress of Immunology
  • Place of Presentation: イタリア･ミラノ
• [Presentation] Generation of polyreactive B cells during murine herpesvirus infection. (2013)
  • Author(s): ○Shuhei Sakakibara, Teruhito Yasui, Takeharu Minamitani, and Hitoshi Kikutani
  • Organizer: Annual Meeting of Japanese Society of Immunology （第４２回日本免疫学会学術集会）
  • Place of Presentation: 千葉･幕張
• [Presentation] Direct and indirect inhibition of germinal cnter formation and humoral immune responses by Epstein-Barr virus LMP1. (2013)
  • Author(s): ○Tsai, C.-Y., T. Yasui, T. Minamitani, S. Sakakibara, and H. Kikutani
  • Organizer: Annual Meeting of Japanese Society of Immunology （第４２回日本免疫学会学術集会）
  • Place of Presentation: 千葉･幕張
• [Presentation] Epstein-Barr virus LMP2a reduces the stringency of B cell selection in germinal center by augmenting BCR signal strength and enhancing plasma cell differentiation. (2013)
  • Author(s): ○Minamitani, T., T. Yasui, S. Sakakibara, C.-Y. Tsai, and H. Kikutani
  • Organizer: Annual Meeting of Japanese Society of Immunology （第４２回日本免疫学会学術集会）
  • Place of Presentation: 千葉･幕張
• [Presentation] Signal-cell biology技術を用いたヒト抗HSV1抗体の単離‐持続感染ウイルスに対するヒト液性免疫応答の推移。 (2013)
  • Author(s): ○安居輝人、渡部明子、榊原修平、義江修、菊谷仁
  • Organizer: 第61回日本ウイルス学会
  • Place of Presentation: 兵庫･神戸
• [Presentation] ガンマヘルペスウイルス感染による自己抗体産生 (2012)
  • Author(s): 榊原修平、安居輝人、南谷武春、菊谷仁
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪市(口頭
• [Presentation] Generation of virus- and self-reactive B cells during herpesvirus infection (2012)
  • Author(s): Sakakibara, S. , T. Yasui, T. Minamitani, H. Kikutani
  • Organizer: The 12th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 兵庫県淡路市(ポスター)
• [Presentation] Generation of virus- and self-reactive B cells during herpesvirus infection (2012)
  • Author(s): Shuhei Sakakibara, Teruhito Yasui, Takeharu Minamitani, Hitoshi Kikutani
  • Organizer: The 11th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 兵庫県淡路市
• [Presentation] 榊原 修平、安居 輝人、南谷 武春、菊谷 仁 (2012)
  • Author(s): ガンマヘルペスウイルス感染による自己抗体産生
  • Organizer: 第６０回 日本ウイルス学会
  • Place of Presentation: 大阪府大阪市
• [Presentation] Shuhei Sakakibara, Teruhito Yasui, Takeharu Minamitani, Hitoshi Kikutani (2012)
  • Author(s): Generation and selection of virus- and self-reactive B cells during herpesvirus infection
  • Organizer: 第41回 日本免疫学会
  • Place of Presentation: 兵庫県神戸市