Development of a rapid GC-MS analysis method for drugs in blood using solid-phase microextraction and high temperature vaporization.

• Project/Area Number: 24790647
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Legal medicine
• Research Institution: Fukuoka University
• Principal Investigator: WATERS Brian 福岡大学, 医学部, 助教 (00609480)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 乱用薬物 / 覚せい剤 / バルビツレート / ベンゾジアゼピン / ヘッドスペース / 固相マイクロ抽出 / ガスクロマトグラフィー / Drugs of abuse / Amphetamines / Barbiturates / Benzodiazepines / Headspace / SPME / Fast GC-MS/MS / methamphetamine / amphetamine / headspace / fast GC-MS/MS

Research Abstract

In this study, a rapid method for analyzing a number of drugs of abuse from whole blood was developed. The goal was to accomplish extraction of the compounds while avoiding harmful organic solvents and tedius solid-phase extraction media. Solid-phase microextraction (SPME) techniques were employed to accomplish this. Initially, the focus was on amphetamine and methamphetamine. Analysis was achieved with pre-treatment of 0.1 g of sample with 0.1 ml of a strong base (5N NaOH). It was also determined that heating the headspace to a temperature of 220C was ideal for adsorption to the SPME fiber. Finally, the exposure time of the fiber to the heated headspace was examined. It was determined that exposure of the SPME fiber to the headspace for 7 minutes was ideal. These conditions were then used to analyze barbiturates and benzodiazepines in whole blood, however, analysis by SPME was problematic. Further study of SPME analysis of these classes of drugs in whole blood is necessary.

Research Products

• [Presentation] A fast and simple method for analyzing drugs of abuse in whole blood using high temperature vaporization and solid-phase microextraction (2014)
  • Author(s): WATERS Brian, Fujii Hiroshi, Hara Kenji, Matsusue Aya, Kashiwagi Masayuki, Kubo Shin-ichi
  • Organizer: 9^<th> International Symposium on Advances in Legal Medicine (ISALM)
  • Place of Presentation: 福岡県、福岡市、日本
  • Year and Date: 2014-06-20
• [Presentation] A fast and simple method for analyzing drugs of abuse in whole blood using high temperature vaporization and solid-phase microextraction. (2014)
  • Author(s): Brian Waters, Hiroshi Fujii, Kenji Hara, Aya Matsusue, Masayuki Kashiwagi, Shin-ichi Kubo
  • Organizer: The 9th International Symposium on Advances in Legal Medicine
  • Place of Presentation: 福岡県福岡市
• [Presentation] Fujii Hiroshi, Kubo Shin-ichi, Optimization of a Method for the Quantification of Underivatized Amphetamines in Whole Blood Using Headspace Solid-Phase Microextraction and Fast GC-MS/MS (2013)
  • Author(s): WATERS Brian, Hara Kenji, Kashiwagi Masayuki, Matsusue Aya, Takayama Mio
  • Organizer: The 43^<rd> Annual Meeting of the Society of Forensic Toxicologists (SOFT)
  • Place of Presentation: Orlando, Florida (USA)
  • Year and Date: 2013-10-30
• [Presentation] Optimization of a Method for the Quantification of Underivatized Amphetamines in Whole Blood Using Headspace Solid-Phase Microextraction and Fast GC-MS/MS (2013)
  • Author(s): Brian Waters, Kenji Hara, Masayuki Kashiwagi, Aya Matsusue, Mio Takayama, Hiroshi Fujii, Shin-ichi Kubo
  • Organizer: Society Of Forensic Toxicologists (SOFT) 2013 Annual Meeting
  • Place of Presentation: Orlando, Florida, USA