Stem cell hierarchy in human intestinal epithelium and colorectal cancer.

• Project/Area Number: 24790718
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Keio University
• Principal Investigator: MATANO mami 慶應義塾大学, 医学部, 研究員 (20439889)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 大腸癌 / 幹細胞 / LGR5

Research Abstract

To elucidate the role of Lgr5 stem cells in colorectal cancer, we attempted to knock-in fluorescence reporter and CreER (liniage tracer) in an Lgr5 locus. Taking advantage of genome editing system, CRISPR/Cas9, we found an optimal condition to engineer human intestinal organoids. With this system, we succeeded in knock-in of CreER into the Lgr5 locus.

Research Products

• [Presentation] ゲノム編集技術による体外における人工的な大腸がんの再構築 (2013)
  • Author(s): 佐藤俊朗,南木康作,藤井正幸,武下達矢,中里圭宏,清野隆史,高野愛,股野麻未,下川真理子,伊達昌一,太田悠木,金井隆典
  • Organizer: 第21回浜名湖シンポジウム
  • Place of Presentation: アクトシティ浜松
  • Year and Date: 2013-12-21
• [Presentation] Molecular mechanism of intestinal stem cell self-renewal : Stem cells and their niche signals. International Symposium 3. Molecular mechanisms for the growth and differentiation of tissue-specific stem cells in mammals (2013)
  • Author(s): Toshiro Sato, Mami Matano, Shoichi Date, Ai Takano, Mariko Shimokawa
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2013-09-11
• [Book] がん基盤生物学―革新的シーズ育成に向けて― (2013)
  • Author(s): 股野麻未、佐藤俊朗
  • Publisher: 南山堂
• [Book] がん基盤生物学―革新的シーズ育成に向けてー (2013)
  • Author(s): 股野麻未, 佐藤俊朗
  • Total Pages: 4
  • Publisher: 南山堂