Analysis of mesenchymal stem cells for vessel formation

• Project/Area Number: 24790735
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Circulatory organs internal medicine
• Research Institution: University of Tsukuba
• Principal Investigator: NAGANO Masumi 筑波大学, 医学医療系, 助教 (30436282)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 間葉系幹細胞 / 閉塞性動脈硬化症 / 血管再生 / 虚血改善 / 細胞治療

Research Abstract

To compare the abilities of MSC, characterize how those MSC affect the repair of ischemic tissue, vascular occlusion was performed by ligation of the femoral artery and vein. Of note, the blood flow in the ischemic region rapidly increased in mice injected with AT-MSC, as contrasted with mice injected with BM- or DT-MSC. The number of CD45+ and F4/80+ cells at the femoral region was higher in AT-MSC recipients than in recipients of BMMSC or DT-MSC. We evaluated the mRNA expression of angiogenic and migration factors in MSC and found the expression of CCL5 mRNA was higher in AT-MSC than in BM-MSC or DT-MSC. Transplantation of ATMSC with impaired expression of CCL5 clearly showed a significant delay in the recovery of blood flow compared with the control.

Research Products

• [Journal Article] The role of CCL5 in the ability of adipose tissue-derived mesenchymal stem cells to support repair of ischemic regions. (2014)
  • Author(s): Kimura K, Nagano M, Salazar G, Yamashita T, Tsuboi I, Mishima H, Matsushita S, Sato F, Yamagata K, Ohneda O.
  • Journal Title: Stem Cells Dev. Volume: 23(5) Issue: 5 Pages: 488-501
  • DOI: 10.1089/scd.2013.0307
  • Peer Reviewed
• [Journal Article] The role of CCL5 in the ability of adipose tissue-derived mesenchymal stem cells to support repair of ischemic regions. (2014)
  • Author(s): Kimura K, Nagano M, Salazar G, Yamashita T, Tsuboi I, Mishima H, Matsushita S, Sato F, Yamagata K, Ohneda O.
  • Journal Title: Stem Cells Dev. Volume: 23 Pages: 488-501
  • Peer Reviewed
• [Journal Article] Establishment of erythroleukemic GAK14 cells and characterization of GATA1 N-terminal domain (2013)
  • Author(s): Mukai HY, Suzuki M, Nagano M, Ohmori S, Otsuki A, Tsuchida K, Moriguchi T, Ohneda K, Shimizu R, Ohneda O, Yamamoto M
  • Journal Title: Genes Cells Volume: 18 Pages: 886-98
  • Peer Reviewed
• [Journal Article] Functional endothelial progenitor cells selectively recruit neurovascular protective monocyte-derived F4/80(+) /Ly6c(+) macrophages in a mouse model of retinal degeneration (2013)
  • Author(s): Fukuda S, Nagano M, Yamashita T, Kimura K, Tsuboi I, Salazar G, Ueno S, Kondo M, Kunath T, Oshika T, Ohneda O
  • Journal Title: Stem Cells Volume: 31 Issue: 10 Pages: 2149-2161
  • DOI: 10.1002/stem.1469
  • Peer Reviewed
• [Journal Article] Umbilical cord blood-derived mesenchymal stem cells inhibit, but adipose tissue-derived mesenchymal stem cells promote, glioblastoma multiforme proliferation (2013)
  • Author(s): Akimoto K, Kimura K, Nagano M, Takano S, To'a Salazar G, Yamashita T, Ohneda O.
  • Journal Title: Stem Cells Dev Volume: 22 Issue: 9 Pages: 1370-1386
  • DOI: 10.1089/scd.2012.0486
  • Peer Reviewed
• [Journal Article] Establishment of erythroleukemic GAK14 cells and characterization of GATA1 N-terminal domain (2013)
  • Author(s): Mukai HY, Suzuki M, Nagano M, Ohmori S, Otsuki A, Tsuchida K, Moriguchi T, Ohneda K, Shimizu R, Ohneda O, Yamamoto M,
  • Journal Title: Genes Cells Volume: 18 Issue: 10 Pages: 886-898
  • DOI: 10.1111/gtc.12084
  • Peer Reviewed
• [Journal Article] A subpopulation of endothelial progenitor cells with low aldehyde dehydrogenase activity attenuates acute ischemic brain injury in rats. (2012)
  • Author(s): Kazuhiro Nakamura, Hideo Tsurushima, Aiki Marushima, Masumi Nagano, Toshiharu Yamashita, Kensuke Suzuki, Osamu Ohneda, Akira Matsumura.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 418 Issue: 1 Pages: 87-92
  • DOI: 10.1016/j.bbrc.2011.12.139
  • NAID: 120007130720
  • Peer Reviewed
• [Presentation] ヒト間葉系幹細胞のグリオブラストーマ増殖抑制に対する作用機構解明 (2013)
  • Author(s): 秋本恵子、木村健一,長野真澄,山下年晴,高野晋吾,大根田修
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: パシフィコ横浜横浜
  • Year and Date: 2013-03-21
• [Presentation] ステロイド薬投与による脂肪組織由来間葉系幹細胞への影響 (2013)
  • Author(s): 加藤俊貴,木村健一,長野真澄,山下年晴,秋本恵子,三島初,大根田修
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: パシフィコ横浜横浜
  • Year and Date: 2013-03-21
• [Presentation] 肺由来血管内皮細胞における低酸素応答転写因子HIF-3aの機能解析 (2012)
  • Author(s): 小林里美,山下年晴,長野真澄,木村健一,大根田修
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡 福岡
  • Year and Date: 2012-12-12
• [Presentation] Hypoxia inducible factor-3a regulates its target genes in association with HIF-1a and HIF-2a. (2012)
  • Author(s): 小林里美,山下年晴,長野真澄,木村健一,坪井一輝,大根田修
  • Organizer: 第10回がんとハイポキシア研究会
  • Place of Presentation: 横浜開港記念館 横浜
  • Year and Date: 2012-12-06
• [Presentation] HIF-1a (2012)
  • Author(s): 白石章,木村健一,長野真澄,山下年晴,坂東裕子,原尚人,大根田修
  • Organizer: 第10回がんとハイポキシア研究会
  • Place of Presentation: 横浜開港記念館 横浜
  • Year and Date: 2012-12-06
• [Presentation] Deficient expression of HIF-2a in endothelial cell cause upregulation of HIF-1a and compensatory angiogenesis.,expression in ALDH-positive breast cancer is involved in cancer progression. (2012)
  • Author(s): 坪井一輝,山下年晴,木村健一,小林里美,長野真澄,大根田修
  • Organizer: 第10回がんとハイポキシア研究会
  • Place of Presentation: 横浜開港記念館 横浜
  • Year and Date: 2012-12-06
• [Presentation] ステロイド薬投与による脂肪組織由来間葉系幹細胞への影響
  • Author(s): 加藤俊貴，木村健一，長野真澄，山下年晴，秋本恵子，三島初，大根田修
  • Organizer: 日本再生医療学会総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] ヒト間葉系幹細胞のグリオブラストーマ増殖抑制に対する作用機構解明
  • Author(s): 秋本恵子，木村健一，長野真澄，高野晋吾，山下年晴，大根田修
  • Organizer: 日本再生医療学会総会
  • Place of Presentation: パシフィコ横浜
• [Remarks]
  • URL: http://www.md.tsukuba.ac.jp/basic-med/remed/stemcell/index.html