• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of mesenchymal stem cells for vessel formation

Research Project

Project/Area Number 24790735
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Circulatory organs internal medicine
Research InstitutionUniversity of Tsukuba

Principal Investigator

NAGANO Masumi  筑波大学, 医学医療系, 助教 (30436282)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords間葉系幹細胞 / 閉塞性動脈硬化症 / 血管再生 / 虚血改善 / 細胞治療
Research Abstract

To compare the abilities of MSC, characterize how those MSC affect the repair of ischemic tissue, vascular occlusion was performed by ligation of the femoral artery and vein. Of note, the blood flow in the ischemic region rapidly increased in mice injected with AT-MSC, as contrasted with mice injected with BM- or DT-MSC. The number of CD45+ and F4/80+ cells at the femoral region was higher in AT-MSC recipients than in recipients of BMMSC or DT-MSC. We evaluated the mRNA expression of angiogenic and migration factors in MSC and found the expression of CCL5 mRNA was higher in AT-MSC than in BM-MSC or DT-MSC. Transplantation of ATMSC with impaired expression of CCL5 clearly showed a significant delay in the recovery of blood flow compared with the control.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (16 results)

All 2014 2013 2012 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (8 results) Remarks (1 results)

  • [Journal Article] The role of CCL5 in the ability of adipose tissue-derived mesenchymal stem cells to support repair of ischemic regions.2014

    • Author(s)
      Kimura K, Nagano M, Salazar G, Yamashita T, Tsuboi I, Mishima H, Matsushita S, Sato F, Yamagata K, Ohneda O.
    • Journal Title

      Stem Cells Dev.

      Volume: 23(5) Issue: 5 Pages: 488-501

    • DOI

      10.1089/scd.2013.0307

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The role of CCL5 in the ability of adipose tissue-derived mesenchymal stem cells to support repair of ischemic regions.2014

    • Author(s)
      Kimura K, Nagano M, Salazar G, Yamashita T, Tsuboi I, Mishima H, Matsushita S, Sato F, Yamagata K, Ohneda O.
    • Journal Title

      Stem Cells Dev.

      Volume: 23 Pages: 488-501

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of erythroleukemic GAK14 cells and characterization of GATA1 N-terminal domain2013

    • Author(s)
      Mukai HY, Suzuki M, Nagano M, Ohmori S, Otsuki A, Tsuchida K, Moriguchi T, Ohneda K, Shimizu R, Ohneda O, Yamamoto M
    • Journal Title

      Genes Cells

      Volume: 18 Pages: 886-98

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional endothelial progenitor cells selectively recruit neurovascular protective monocyte-derived F4/80(+) /Ly6c(+) macrophages in a mouse model of retinal degeneration2013

    • Author(s)
      Fukuda S, Nagano M, Yamashita T, Kimura K, Tsuboi I, Salazar G, Ueno S, Kondo M, Kunath T, Oshika T, Ohneda O
    • Journal Title

      Stem Cells

      Volume: 31 Issue: 10 Pages: 2149-2161

    • DOI

      10.1002/stem.1469

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Umbilical cord blood-derived mesenchymal stem cells inhibit, but adipose tissue-derived mesenchymal stem cells promote, glioblastoma multiforme proliferation2013

    • Author(s)
      Akimoto K, Kimura K, Nagano M, Takano S, To'a Salazar G, Yamashita T, Ohneda O.
    • Journal Title

      Stem Cells Dev

      Volume: 22 Issue: 9 Pages: 1370-1386

    • DOI

      10.1089/scd.2012.0486

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Establishment of erythroleukemic GAK14 cells and characterization of GATA1 N-terminal domain2013

    • Author(s)
      Mukai HY, Suzuki M, Nagano M, Ohmori S, Otsuki A, Tsuchida K, Moriguchi T, Ohneda K, Shimizu R, Ohneda O, Yamamoto M,
    • Journal Title

      Genes Cells

      Volume: 18 Issue: 10 Pages: 886-898

    • DOI

      10.1111/gtc.12084

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A subpopulation of endothelial progenitor cells with low aldehyde dehydrogenase activity attenuates acute ischemic brain injury in rats.2012

    • Author(s)
      Kazuhiro Nakamura, Hideo Tsurushima, Aiki Marushima, Masumi Nagano, Toshiharu Yamashita, Kensuke Suzuki, Osamu Ohneda, Akira Matsumura.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 418 Issue: 1 Pages: 87-92

    • DOI

      10.1016/j.bbrc.2011.12.139

    • NAID

      120007130720

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] ヒト間葉系幹細胞のグリオブラストーマ増殖抑制に対する作用機構解明2013

    • Author(s)
      秋本恵子、木村健一,長野真澄,山下年晴,高野晋吾,大根田修
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜横浜
    • Year and Date
      2013-03-21
    • Related Report
      2013 Final Research Report
  • [Presentation] ステロイド薬投与による脂肪組織由来間葉系幹細胞への影響2013

    • Author(s)
      加藤俊貴,木村健一,長野真澄,山下年晴,秋本恵子,三島初,大根田修
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜横浜
    • Year and Date
      2013-03-21
    • Related Report
      2013 Final Research Report
  • [Presentation] 肺由来血管内皮細胞における低酸素応答転写因子HIF-3aの機能解析2012

    • Author(s)
      小林里美,山下年晴,長野真澄,木村健一,大根田修
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡 福岡
    • Year and Date
      2012-12-12
    • Related Report
      2013 Final Research Report
  • [Presentation] Hypoxia inducible factor-3a regulates its target genes in association with HIF-1a and HIF-2a.2012

    • Author(s)
      小林里美,山下年晴,長野真澄,木村健一,坪井一輝,大根田修
    • Organizer
      第10回がんとハイポキシア研究会
    • Place of Presentation
      横浜開港記念館 横浜
    • Year and Date
      2012-12-06
    • Related Report
      2013 Final Research Report
  • [Presentation] HIF-1a2012

    • Author(s)
      白石章,木村健一,長野真澄,山下年晴,坂東裕子,原尚人,大根田修
    • Organizer
      第10回がんとハイポキシア研究会
    • Place of Presentation
      横浜開港記念館 横浜
    • Year and Date
      2012-12-06
    • Related Report
      2013 Final Research Report
  • [Presentation] Deficient expression of HIF-2a in endothelial cell cause upregulation of HIF-1a and compensatory angiogenesis.,expression in ALDH-positive breast cancer is involved in cancer progression.2012

    • Author(s)
      坪井一輝,山下年晴,木村健一,小林里美,長野真澄,大根田修
    • Organizer
      第10回がんとハイポキシア研究会
    • Place of Presentation
      横浜開港記念館 横浜
    • Year and Date
      2012-12-06
    • Related Report
      2013 Final Research Report
  • [Presentation] ステロイド薬投与による脂肪組織由来間葉系幹細胞への影響

    • Author(s)
      加藤俊貴,木村健一,長野真澄,山下年晴,秋本恵子,三島初,大根田修
    • Organizer
      日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] ヒト間葉系幹細胞のグリオブラストーマ増殖抑制に対する作用機構解明

    • Author(s)
      秋本恵子,木村健一,長野真澄,高野晋吾,山下年晴,大根田修
    • Organizer
      日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.md.tsukuba.ac.jp/basic-med/remed/stemcell/index.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi