Epigenetic regulation induces the functional cardiomyocytes derived from human pluripotent stem cells
Project/Area Number |
24790755
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Circulatory organs internal medicine
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Research Institution | Kyoto University |
Principal Investigator |
OTSUJI Tomomi G. 京都大学, 物質-細胞統合システム拠点, 研究員 (50564754)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | 移植・再生医療 / ヒトES/iPS細胞 / 心筋分化 / 心機能 / 機能細胞 / 大量培養法 |
Outline of Final Research Achievements |
The cardiomyocytes (CMs) derived from Human ESC and iPSC (hESC/iPSC) are currently viewed as promising tools in regenerative medicine and pharmacological development. However, they are functionally heterogeneous, display insufficient biological efficacy and generally possess the electrophysiological properties seen in fetal CMs. Here, treating with a histone deacetylase inhibitor (HDACi) facilitates cardiac function in primitive hESC/hiPSC-CMs. HDACi-treated hESC/hiPSC-CM colonies showed appropriate responses to particular concentrations of known potassium ion channel inhibitors. In addition, we developed 3D sphere culture system without mechanical stirring for hESC/hiPSCs. Combination of these system may be optimized toward translation into a large-scale hESC/iPSC-CMs production format.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] A 3D Sphere Culture System Containing Functional Polymers for Large-scale Human Pluripotent Stem Cell Production2014
Author(s)
Tomomi G Otsuji, Jiang Bin, Azumi Yoshimura, Misayo Tomura, Daiki Tateyama, Itsunari Minami, Yoshihiro Yoshikawa, Kazuhiro Aiba, John E Heuser, Taito Nishino, Kouichi Hasegawa, Norio Nakatsuji
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Journal Title
Stem Cell Reports
Volume: 2
Issue: 5
Pages: 734-745
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A small molecule that promotes cardiac differentiation of human pluripotent stem cells under defined, cytokine- and xeno-free conditions2012
Author(s)
Minami I, Yamada K, Otsuji TG, Yamamoto T, Shen Y, Otsuka S, Kadota S, Morone N, Barve M, Asai Y, Tenkova-Heuser T, Heuser JE, Uesugi M, Aiba K, Nakatsuji N
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Journal Title
Cell Rep
Volume: 2(5)
Issue: 5
Pages: 1448-60
DOI
Related Report
Peer Reviewed
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[Presentation] 3D sphere culture with functional polymers for large-scale human pluripotent stem cell production2014
Author(s)
Tomomi G. Otsuji, Jiang Bin, Azumi Yoshimura, Misayo Tomura, Daiki Tateyama, Itsunari Minami, Yoshihiro Yoshikawa, Kazuhiro Aiba, John E. Heuser, Taito Nishino, Kouichi Hasegawa, Norio Nakatsuji
Organizer
ISSCR 12th
Place of Presentation
Canada, Vancouver
Year and Date
2014-06-18
Related Report
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[Presentation] 3D sphere culture with functional polymers for large-scale human pluripotent stem cell production2014
Author(s)
Tomomi G Otsuji, Jiang Bin, Azumi Yoshimura, Misayo Tomura, Daiki Tateyama, Itsunari Minami, Yoshihiro Yoshikawa, Kazuhiro Aiba, John E Heuser, Taito Nishino, Kouichi Hasegawa, Norio Nakatsuji
Organizer
日本再生医療学会 第13回総会
Place of Presentation
京都市
Related Report
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