MicroRNA expression in plasma and new therapy for cardiovascular diseases

• Project/Area Number: 24790766
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Circulatory organs internal medicine
• Research Institution: Kyushu University
• Principal Investigator: TOKUNOU Tomotake 九州大学, 医学(系)研究科(研究院), 助教 (50567378)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 分子血管病態学 / マイクロRNA / 低酸素

Research Abstract

Sleep apnea syndrome is one of the risks of cardiovascular diseases. MicroRNAs from plasma of sleep apnea syndrome patients with agreement of using CPAP (continuous positive airway pressure) were measured. The microRNAs expression was measured before and after CPAP therapy. First microRNAs expression was measured by microRNA array. The result showed some microRNAs expression changed after CPAP therapy. The expression changes of microRNAs were measured by real-time PCR with 11 samples, but the result showed no significant difference with CPAP therapy in this case group.
The expression of microRNA-132 of vascular endothelial cells decreased 0.52 times with low oxygen culture condition. One of the microRNA-132 targets is p120RasGAP (Ras GTPase activating protein). The administration of microRNA-132 might be one of the therapeutic targets of ischemic vascular diseases by decrease of p120RasGAP. But the delivery method of microRNA is needed to be improved.

Research Products

• [Journal Article] Stimulation of α7 nicotinic acetylcholine receptor by AR-R17779 suppresses atherosclerosis and aortic aneurysm formation in apolipoprotein E-deficient mice. (2014)
  • Author(s): Hashimoto T, Ichiki T, Watanabe A, Hurt-Camejo E, Michaëlsson E, Ikeda J, Inoue E, Matsuura H, Tokunou T, Kitamoto S, Sunagawa K.
  • Journal Title: Vascul Pharmacol Volume: In press Issue: 2-3 Pages: 49-55
  • DOI: 10.1016/j.vph.2014.03.006
  • Peer Reviewed / Open Access
• [Journal Article] Suppression of abdominal aortic aneurysm formation by inhibition of prolyl hydroxylase domain protein through attenuation of inflammation and extracellular matrix disruption. (2014)
  • Author(s): Watanabe A, Ichiki T, Sankoda C, Takahara Y, Ikeda J, Inoue E, Tokunou T, Kitamoto S, Sunagawa K.
  • Journal Title: Clin Sci Volume: 126 Issue: 9 Pages: 671-678
  • DOI: 10.1042/cs20130435
  • Peer Reviewed / Open Access
• [Journal Article] Deletion of phd2 in myeloid lineage attenuates hypertensive cardiovascular remodeling. (2013)
  • Author(s): Ikeda J, Ichiki T, Matsuura H, Inoue E, Kishimoto J, Watanabe A, Sankoda C, Kitamoto S, Tokunou T, Takeda K, Fong GH, Sunagawa K.
  • Journal Title: J Am Heart Assoc. Volume: 2 Issue: 3
  • DOI: 10.1161/jaha.113.000178
  • Peer Reviewed
• [Journal Article] Comparison of pre-dilation with a non-compliant balloon versus a dual wire scoring balloon for coronary stenting (2013)
  • Author(s): Sadamatsu K, Yoshida K, Yoshidomi Y, Koga Y, Amari K, Tokunou T
  • Journal Title: World Journal of Cardiovascular Diseases Volume: 3 Issue: 06 Pages: 395-400
  • DOI: 10.4236/wjcd.2013.36061
  • Peer Reviewed
• [Journal Article] Comparison of pre-dilation with a non-compliant balloon versus a dual wire scoring balloon for coronary stenting. (2013)
  • Author(s): Sadamatsu K, Yoshida K, Yoshidomi Y, Koga Y, Amari K, Tokunou T.
  • Journal Title: World Journal of Cardiovascular Diseases, Volume: 3 Pages: 395-400
  • Peer Reviewed
• [Journal Article] Recurrence of Takotsubo cardiomyopathy with coronary slow flow phenomenon (2012)
  • Author(s): Tokunou T, Sadamatsu K
  • Journal Title: Journal of Cardiology Cases Volume: 5 Issue: 2 Pages: e100-e106
  • DOI: 10.1016/j.jccase.2011.10.003
• [Journal Article] Resveratrol attenuates angiotensin II-induced senescence of vascular smooth muscle cells. (2012)
  • Author(s): Ichiki T
  • Journal Title: Regulatory Peptide Volume: 177 Issue: 1-3 Pages: 35-39
  • DOI: 10.1016/j.regpep.2012.04.005
  • Peer Reviewed
• [Presentation] Suppression of Abdominal Aortic Aneurysm Formation by Inhibition of Prolyl Hydroxylase Domain Protein through Attenuation of Inflammation and Extracellular Matrix Disruption. (2014)
  • Author(s): Aya Watanabe, Toshihiro Ichiki, Chikahiro Sankoda, Yusuke Takahara, Jiro Ikeda, Eriko Inoue, Tomotake Tokunou, Shiro Kitamoto, Kenji Sunagawa
  • Organizer: 第78回日本循環器学会
  • Place of Presentation: 東京
  • Year and Date: 2014-03-23
• [Presentation] Suppression of Abdominal Aortic Aneurysm Formation by Inhibition of Prolyl Hydroxylase Domain Protein through Attenuation of Inflammation and Extracellular Matrix Disruption. (2014)
  • Author(s): Aya Watanabe, Toshihiro Ichiki, Chikahiro Sankoda, Yusuke Takahara, Jiro Ikeda, Eriko Inoue, Tomotake Tokunou, Shiro Kitamoto, Kenji Sunagawa.
  • Organizer: 第78回日本循環器学会学術大会
  • Place of Presentation: 東京
• [Presentation] New Strategies for Patients with Atrial Septal Defect and Severe Pulmonary Arterial Hypertension : Combination of Medical Therapy and Catheter Closure (2013)
  • Author(s): Daisuke Toyomura, Teiji Akagi, Yasufumi Kijima, Koji Nakagawa, Kengo Kusano, Toshiro Shinke, Atsushi Yao, Tomotake Tokunou, Hiromi Matsubara, Katsumasa Miyaji, Hiroshi Itoh, Shunji Sano
  • Organizer: 日本循環器学会
  • Place of Presentation: 横浜
  • Year and Date: 2013-03-15
• [Presentation] 重度肺高血圧を伴う心房中隔欠損症に対する新しい治療戦略 (2013)
  • Author(s): 赤木禎治、木島康文、中川晃志、松原広己、得能智武、新家俊郎、八尾厚史、伊藤浩、佐野俊二
  • Organizer: 第61回日本心臓病学会
  • Place of Presentation: 熊本
• [Presentation] 重症肺高血圧症を合併した心房中隔欠損症に対する心房中隔欠損孔閉鎖術の有効性 (2013)
  • Author(s): 得能智武、石北綾子、肥後太基、赤木禎治、砂川賢二
  • Organizer: 第61回日本心臓病学会
  • Place of Presentation: 熊本
• [Remarks]
  • URL: http://www.med.kyushu-u.ac.jp/cardiol/2_junkan/