Detection of Notch1 mutation using PCR-Clamp method

• Project/Area Number: 24790797
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Respiratory organ internal medicine
• Research Institution: Hokkaido University
• Principal Investigator: ASAHINA Hajime 北海道大学, 大学病院, 助教 (90374298)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 肺癌 / Notch

Research Abstract

I tried to develop a highly specific detection system of Notch 1 mutation. To amplify the point mutation of Notch 1, I used the PCR clamp method. First, I tried to a clamp primer which covers the normal DNA of the mutation site. Second, I tried to design a mutant probe which covers the mutation sequence. But we cannot get the adequate primer.