Significance of tissue progenitor cells in progression of lung fibrosis and immunological depletion of progenitor cells

• Project/Area Number: 24790806
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Respiratory organ internal medicine
• Research Institution: Niigata University
• Principal Investigator: SAKAGAMI TAKURO 新潟大学, 医歯学系, 助教 (00444159)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 組織幹細胞 / 間質性肺炎 / 線維化 / 分化 / アポトーシス

Outline of Final Research Achievements

This investigation was performed following the hypothesis that abnormal differentiation of tissue stem cells might be one of the development mechanisms of lung fibrosis.
Creating the mice model which tissue stem cells were depleted immunologicaly. Extracting the tissue stem cells from lung, after apoptosis was induced, it was administered to antigen presenting cells (APC) differentiated from mice bone marrow cells. APCs were administered to mice, then interstitial pneumonia was induced by bleomysine. High mortality rate and delayed recovery from inflammation were revealed in these mice.