Development of pleural mesothelioma treatment targeting for cancer-associated fibroblasts

Research Project

Project/Area Number	24790815
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Respiratory organ internal medicine
Research Institution	Kagawa University
Principal Investigator	KANAJI Nobuhiro 香川大学, 医学部附属病院, 助教 (60403789)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	悪性胸膜中皮腫 / 線維芽細胞
Outline of Final Research Achievements	Most cases of malignant pleural mesothelioma are inoperable, and have poor prognosis when they are chemotherapy-resistance. Recently, cancer-associated fibroblasts (CAF) had been reported, that are observed around cancer cells and have a role on cancer progression. In this study, association between malignant pleural mesothelioma cells and fibroblasts were investigated. Cell migration was assessed by chemotaxis, and cell invasion was assessed by Matrigel invasion assay. The culture media derived from lung fibroblasts augmented magratory and invasive abilities of malignant pelural mesothelioma cells. Exogenous fibronectin and hepatocyte growth factor (HGF) augmented mesothelioma cell migration and invasion, respectively. It was found that fibronectin and HGF were released from lung fibroblasts.