Estimation of effects of EGFR-TKI on NSCLCs by cell-based approach including CTOS
Project/Area Number |
24790829
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Respiratory organ internal medicine
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Research Institution | 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所) |
Principal Investigator |
WATANUKI ZENTA 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん先進治療開発研究部, 特任研究員 (10626518)
|
Project Period (FY) |
2012-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 癌 / 肺癌 / EGFR-TKI / CTOS |
Research Abstract |
EGFR-TKIs work effectively on NSCLCs with EGFR-mutations, however, most of the cases eventually develop drug resistance by acquiring an additional mutation T790M. In the present study, I aimed to establish a CTOS system; a spheroid culture system with patient derived cancer cells. Initial CTOS trial worked fine. Nevertheless, due to the relatively slow rate of tumors, I established another cell-based EGFR-TKI evaluation system. Forced expression of EGFR, either wild type or mutations, enabled K562 cells to grow and survive by the help of the EGFR-driven signals. EGFR-TKI killed the cells, in a dose-dependent manner. Among the genotypes tested, L858R was the most sensitive, whereas L858R/L790M showed resistance to the first generation TKIs. I found that EGFR-TKI-induced cell death was correlated with BIMEL induction and autophagy.
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Report
(3 results)
Research Products
(2 results)
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[Presentation] A Case of Small Cell Lung Cancer with Cancer-Associated Retinopathy (CAR) Accompanied by Circulating Anti-CRMP5/CV2 Antibodies2013
Author(s)
Mami Morita, Tatsuro Fukuhara, Yoko Tsukita, Zenta Watanuki, Aya Suzuki, Kana Watanabe, Nobuyuki Sato, Hidetoshi Takahashi, Shigemi Ito, Ikuro Sato, and Makoto Maemondo
Organizer
18th Congress of the Asian Pacific Society of Respirology
Place of Presentation
Yokohama
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