Roles of PDIP1, a cofactor for PPARg, in adipocytes

• Project/Area Number: 24790906
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Metabolomics
• Research Institution: Gunma University
• Principal Investigator: TOMARU Takuya 群馬大学, 医学部附属病院, その他 (70594399)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 脂肪細胞 / PPARg / 転写共役因子 / PDIP1

Research Abstract

Obesity increases risk of developing diabetes mellitus, hypertension and dyslipidemia, which finally leads to life-threating disease such as myocardial or cerebral infarction. PPARg has been shown to a master regulator of adiogenesis. We recently identified PDIP1 as a cofactor for PPARg. In this study we made PDIP1-depleted preadipocytes using RNA interfering method, and subjected them to differentiation. PDIP1-depleted adipocytes had less lipid droplets, and lower adipocyte-specific gene expression compared to control cells. These results suggest that PDIP1 is required for adipogenesis. We also compared the gene expression profile of fat tissue of PDIP1-depleted mice and that of wild type mice in genome-wide manner, and identified PDIP1-regulated genes.

Research Products

• [Journal Article] A case of thyroid storm with markedly elevated level of circulating soluble interleukin-2 receptor complicated by multiple organ failure and disseminated intravascular coagulation syndrome (2014)
  • Author(s): Shimoda Y, Satoh T, Takahashi H, Katano-Toki A, Ozawa A, Tomaru T, Horiguchi K, Kaira K, Nishioka M, Shibusawa N, Hashimoto K, Wakino S, Mori M, Yamada M
  • Journal Title: Endocr J Volume: (Epub ahead of print)
  • NAID: 130004677940
  • Peer Reviewed
• [Journal Article] A case of thyroid storm with a markedly elevated level of circulating soluble interleukin-2 receptor complicated by multiple organ failure and disseminated intravascular coagulation syndrome (2014)
  • Author(s): Shimoda Y, Satoh T, Takahashi H, Katano-Toki A, Ozawa A, Tomaru T, Horiguchi N, Kaira K, Nishioka M, Shibusawa N, Hashimoto K, Wakino S, Mori M, Yamada M
  • Journal Title: Endocr J. Volume: epub ahead of print
  • NAID: 130004677940
  • Peer Reviewed
• [Journal Article] THRAP3 interacts with HELZ2 and plays a novel role in adipocyte differentiation. (2013)
  • Author(s): Katano-Toki A, Satoh T, Tomaru T, Yoshino S, Ishizuka T, Ishii S, Ozawa A, Shibusawa N, Tsuchiya T, Saito T, Shimizu H, Hashimoto K, Okada S, Yamada M, Mori M
  • Journal Title: Molecular Endocrinology Volume: 27 Issue: 5 Pages: 769-780
  • DOI: 10.1210/me.2012-1332
  • Peer Reviewed
• [Journal Article] KCNJ5 mutations in aldosterone- and cortisol-co-secreting adrenal adenomas (2013)
  • Author(s): Yamada M, Nakajima Y, Taguchi R, Okamura T, Ishii S, Tomaru T, Ozawa A, Shibusawa N, Yoshino S, Toki A, Ishida E, Ozawa A, Hashimoto K, Satoh T, Mori M
  • Journal Title: Endocr J Volume: 59(8) Pages: 735-41
  • NAID: 10031064823
  • URL: https://www.jstage.jst.go.jp/article/endocrj/59/8/59_EJ12-0247/_article
  • Peer Reviewed
• [Journal Article] A novel adipose-specific gene deletion model demonstrates potential pitfalls of existing methods (2013)
  • Author(s): Mullican SE, Tomaru T, Gaddis CA, Peed LC, Sundaram A, Lazar MA
  • Journal Title: Mol Endocrinol Volume: 27(1) Issue: 1 Pages: 127-34
  • DOI: 10.1210/me.2012-1267
  • Peer Reviewed
• [Journal Article] KCNJ5 mutations in aldosterone- and cortisol-co-secreting adrenal adenomas. (2013)
  • Author(s): Yamada M, Nakajima Y, Taguchi R, Okamura T, Ishii S, Tomaru T, Ozawa A, Shibusawa N, Yoshino S, Toki A, Ishida E, Hashimoto K, Satoh T, Mori M.
  • Journal Title: Endocr J. Volume: 59(8) Pages: 735-41
  • NAID: 10031064823
  • Peer Reviewed
• [Journal Article] A novel adipose-specific gene deletion model demonstrates potential pitfalls of existing methods. (2013)
  • Author(s): Mullican SE, Tomaru T, Gaddis CA, Peed LC, Sundaram A, Lazar MA.
  • Journal Title: Mol Endocrinol Volume: 27(1) Pages: 127-34
  • Peer Reviewed
• [Journal Article] NR4A1 (Nur77) mediates thyrotropin-releasing hormone-induced stimulation of transcription of the thyrotropin β gene: analysis of TRH knockout mice. (2012)
  • Author(s): Nakajima Y
  • Journal Title: PLos One Volume: 7 Issue: 7 Pages: e40437-e40437
  • DOI: 10.1371/journal.pone.0040437
  • Peer Reviewed
• [Presentation] Roles of Helz2, helicase with zinc finger 2, in the maturation of white adipocytes (2013)
  • Author(s): Takuya Tomaru, Tetsurou Satoh, Satoshi Yoshino, Akiko Katano-Toki, Takahiro Ishizuka, Yasuyo Nakajima, Sumiyasu Ishii, Atsushi Ozawa, Nobuyuki Shibusawa, Koshi Hashimoto, Shuichi Okada, Masanobu Yamada, Masatomo Mori
  • Organizer: Cold Spring Harbor Laboratory meeting, Metabolic Signaling & Disease : From Cell to Organism
  • Place of Presentation: Long Island, NY
• [Presentation] Protection against high-fat diet induced obesity in Helz2-deficient mice by enhancing hepatic leptin sensitivity, despite central leptin reisistance (2013)
  • Author(s): Satoshi Yoshino, Tetsurou Satoh, Masanobu Yamada, Takuya Tomaru, Akiko Katano-Toki, Satoru Kakizaki, Hayato Ikota, munemasa Mori, Koshi Hashimoto, Atsushi Ozawa, Shuichi Okada, Yoichi Nakazato, Takashi Matozaki, Tsutomu Sasaki, Tadahiro Kitamura, Masatomo Mori
  • Organizer: Cold Spring Harbor Laboratory meeting, Metabolic Signaling & Disease : From Cell to Organism
  • Place of Presentation: Long Island, NY
• [Presentation] 核内受容体転写共役因子PDIP1 は脂肪細胞分化を制御する (2013)
  • Author(s): 登丸 琢也, 佐藤 哲郎, 片野 明子, 吉野 聡, 石塚 高広, 中島 康代, 石井 角保, 小澤 厚志, 渋沢 信行, 橋本 貢士, 山田 正信, 森 昌朋
  • Place of Presentation: 仙台市
• [Presentation] Roles of Helz2, helicase with zinc finger 2, in the maturation of white adipocytes (2013)
  • Author(s): Takuya Tomaru, Tetsurou Satoh, Satoshi Yoshino, Akiko Katano-Toki, Takahiro Ishizuka, Yasuyo Nakajima, Sumiyasu Ishii, Atsushi Ozawa, Nobuyuki Shibusawa, Koshi Hashimoto, Shuichi Okada, Masanobu Yamada, Masatomo Mori
  • Organizer: Cold Spring Harbor Laboratory Meeting, Metabolic Signaling & Disease: From Cell to Organism
  • Place of Presentation: Cold Spring Harbor, NY, USA
• [Presentation] Protection against high-fat diet induced obesity in Helz2-deficient mice by enhancing hepatic leptin sensitivity, despite central leptin resistance (2013)
  • Author(s): Satoshi Yoshino, Tetsurou Satoh, Masanobu Yamada, Takuya Tomaru, Takahiro Ishizuka, Akiko Katano-Toki, Satoru Kakizaki, Hayato Ikota, Munemasa Mori, Koshi Hashimoto, Atsushi Ozawa, Shuichi Okada, Yoichi Nakazato, Takashi Matozaki, Tsutomu Sasaki, Tadahiro Kitamura, Masatomo Mori
  • Organizer: Cold Spring Harbor Laboratory Meeting, Metabolic Signaling & Disease: From Cell to Organism
  • Place of Presentation: Cold Spring Harbor, NY, USA
• [Presentation] 核内受容体転写共役因子PDIP1は脂肪細胞分化を制御する (2013)
  • Author(s): 登丸琢也、佐藤哲郎、片野明子、吉野聡、石塚高広、中島康代、石井角保、小澤厚志、渋沢信行、橋本貢士、山田正信、森昌朋
  • Organizer: 第８６回 日本内分泌学会学術総会
  • Place of Presentation: 仙台市 仙台国際センター
• [Presentation] レプチン受容体isoform における選択的スプライシング機構の解明 (2012)
  • Author(s): 登丸 琢也, 佐藤 哲郎, 吉野 聡, 片野明子, 石井角保, 小澤 厚志, 渋沢 信行, 橋本 貢士, 山田 正信, 森 昌朋
  • Organizer: 日本肥満学会
  • Place of Presentation: 京都市
• [Presentation] マウスレジスチン遺伝子のスプライスフォームに関する検討 (2012)
  • Author(s): 登丸 琢也, 佐藤 哲郎, 吉野 聡, 片野明子, 小澤 厚志, 渋沢 信行, 橋本 貢士, 山田 正信, 森 昌朋
  • Organizer: 日本糖尿病学会年次学術総会
  • Place of Presentation: 横浜市
• [Presentation] マウスレジスチン遺伝子のスプライスフォームに関する検討 (2012)
  • Author(s): 登丸 琢也, 佐藤 哲郎, 吉野 聡, 片野明子, 小澤 厚志, 渋沢 信行, 橋本 貢士, 山田 正信, 森 昌朋
  • Organizer: 日本内分泌学会学術総会
  • Place of Presentation: 名古屋市
• [Presentation] マウスレジスチン遺伝子のスプライスフォームに関する検討 (2012)
  • Author(s): 登丸琢也、佐藤哲郎、吉野聡、片野明子、小澤厚志、渋沢信行、橋本貢士、山田正信、森昌朋
  • Organizer: 日本内分泌学会学術総会
  • Place of Presentation: 名古屋国際会議場（愛知県）
• [Presentation] マウスレジスチン遺伝子のスプライスフォームに関する検討 (2012)
  • Author(s): 登丸琢也、佐藤哲郎、吉野聡、片野明子、小澤厚志、渋沢信行、橋本貢士、山田正信、森昌朋
  • Organizer: 日本糖尿病学会年次学術集会
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] レプチン受容体isoformにおける選択的スプライシング分子機構の解明 (2012)
  • Author(s): 登丸琢也、佐藤哲郎、吉野聡、片野明子、石塚高広、石井角保、小澤厚志、渋沢信行、橋本貢士、山田正信、森昌朋
  • Organizer: 日本肥満学会
  • Place of Presentation: ホテルグランヴィア京都（京都府）
• [Presentation] 甲状腺転移を来した肺腺癌の1例 (2012)
  • Author(s): 登丸琢也、須賀孝慶、岩崎靖樹、山崎勇一、渋沢信行、石井角保、小澤厚志、橋本貢士、山田正信、森昌朋
  • Organizer: 日本甲状腺学会学術総会
  • Place of Presentation: アクロス福岡（福岡県）