| Project/Area Number |
24790906
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Metabolomics
|
|---|
| Research Institution | Gunma University |
|---|
Principal Investigator |
TOMARU Takuya 群馬大学, 医学部附属病院, その他 (70594399)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 脂肪細胞 / PPARg / 転写共役因子 / PDIP1 |
|---|
| Research Abstract |
Obesity increases risk of developing diabetes mellitus, hypertension and dyslipidemia, which finally leads to life-threating disease such as myocardial or cerebral infarction. PPARg has been shown to a master regulator of adiogenesis. We recently identified PDIP1 as a cofactor for PPARg. In this study we made PDIP1-depleted preadipocytes using RNA interfering method, and subjected them to differentiation. PDIP1-depleted adipocytes had less lipid droplets, and lower adipocyte-specific gene expression compared to control cells. These results suggest that PDIP1 is required for adipogenesis. We also compared the gene expression profile of fat tissue of PDIP1-depleted mice and that of wild type mice in genome-wide manner, and identified PDIP1-regulated genes.
|
