Role of Chitin in Allergic Airway Inflammation

• Project/Area Number: 24791005
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Kyorin University
• Principal Investigator: ARAE Ken 杏林大学, 保健学部, 講師 (50306669)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: アレルギー / キチン / IL-33 / Th2

Research Abstract

Chitin, b-(1-4)-poly-N-acetyl-D-glucosamine, is a major constituent of the exoskeleton of house dust mites (HDM), raising the possibility that it may be involved in the development of HDM-related allergic disorders. However, the role of chitin in the pathogenesis of allergic disorders is poorly understood. Chitin enhances Th2-type immune responses in airway such as eosinophilia, IgE production and Th2-cytokine production dependently on the IL-33 production and IL-4/IL-13-STAT-6 pathway, resulting in aggravated OVA-induced airway inflammation in mice. In addition, chitin-mediated IL-33 activates pulmonary DCs to induce excessive IL-1b production, and then the DCs enhance the activation of Th2 cells dependently on antigen and IL-1 signaling.

Research Products

• [Journal Article] Role of Interleukin-33 in Innate-Type Immune Cells in Allergy (2013)
  • Author(s): Nakae S, Morita H, Ohno T, Arae K, Matsumoto K, Saito H
  • Journal Title: Allergology International Volume: 62 Issue: 1 Pages: 13-20
  • DOI: 10.2332/allergolint.13-RAI-0538
  • NAID: 130004477181
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed
• [Journal Article] Epithelial cell-derived IL-25, but not Th17 cell-derived IL-17 or IL-17F, is crucial for murine asthma (2012)
  • Author(s): Suzukawa M, Morita H, Nambu A, Arae K, Shimura E, Shibui A, Yamaguchi S, Suzukawa K, Nakanishi W, Oboki K, Kajiwara N, Ohno T, Ishii A, Körner H, Cua DJ, Suto H, Yoshimoto T, Iwakura Y, Yamasoba T, Ohta K, Sudo K, Saito H, Okumura K, Broide DH, Matsumoto K, Nakae S
  • Journal Title: J Immunol Volume: 189(7) Pages: 3641-52
  • URL: http://www.jimmunol.org/content/189/7/3641.long
  • Peer Reviewed
• [Journal Article] Interleukin-33 in allergy. (2012)
  • Author(s): Ohno T, Morita H, Arae K, Matsumoto K, Nakae S.
  • Journal Title: Allergy. Volume: 67 Issue: 10 Pages: 1203-14
  • DOI: 10.1111/all.12004
  • Peer Reviewed
• [Journal Article] ST2 Requires Th2-, but Not Th17-, Type Airway Inflammation in Epicutaneously Antigen-Sensitized Mice (2012)
  • Author(s): Morita H, Arae K, Ohno T, Kajiwara N, Oboki, K, Matsuda A, Suto H, Okumura K, Sudo K, Takahashi T, Matsumoto K, Nakae, S
  • Journal Title: Allergology International Volume: 61 Issue: 2 Pages: 265-273
  • DOI: 10.2332/allergolint.11-OA-0379
  • NAID: 130004477129
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed
• [Journal Article] Epithelial cell-derived IL-25, but not Th17 cell-derived IL-17 or IL-17F, is crucial for murine asthma. (2012)
  • Author(s): Suzukawa M, Morita H, Nambu A, Arae K, Shimura E, Shibui A, Yamaguchi S, Suzukawa K, Nakanishi W, Oboki K, Kajiwara N, Ohno T, Ishii A et al.
  • Journal Title: J Immunol. Volume: 189 Pages: 3641-3652
  • Peer Reviewed
• [Presentation] Chitin-mediated IL-33 induces breakdown of immune tolerance by excessive IL-1・ production by DCs, causing aggravation of murine asthma (2013)
  • Author(s): Ken Arae
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張
  • Year and Date: 2013-12-12
• [Presentation] Chitin-mediated IL-33 induces breakdown of immune tolerance by excessive IL-1β production by DCs, causing aggravation of murine asthma. (2013)
  • Author(s): ARAE Ken, TAGUCHI Haruhiko, MATSUMOTO Kenji, NAKAE Susumu
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ（千葉市）
• [Presentation] キチンはIL-33を介してアレルギー性気道炎症を誘導する (2013)
  • Author(s): 新江賢、蔵田訓、神谷茂、田口晴彦
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張