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Proteomics analysis in IgG4-related disease

Research Project

Project/Area Number 24791010
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field 膠原病・アレルギー・感染症内科学
Research InstitutionKanazawa Medical University

Principal Investigator

KAWANAMI Takafumi  金沢医科大学, 医学部, 講師 (20350762)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsIgG4関連疾患 / プロテオミクス / クラススイッチ / Alpha-1 antitrypsin / LRA2G
Outline of Final Research Achievements

We conducted a comprehensive analysis of proteins that change in a disease specific manner, using the serum of patients with IgG4-related disease before and after treatment, and that of healthy individuals, in order to identify proteins that are associated with the pathogenesis of IgG4-related disease. After protein removal as a pretreatment, two-dimensional electrophoresis of the serum was conducted and silver staining method was used. We also cut out the spots which changed before and after treatment and detection was performed by ESI-MS/MS. In addition, we identified proteins whose expression significantly changed before and after treatment, as well as those between healthy individuals and patients and confirmed the results with ELISA. From two IgG4-related disease cases, we extracted 38 proteins which significantly changed expression before and after treatment. We found the increase in inflammatory factors such as Alpha-1 antitrypsin, Leucin-rich alpha-2- glycoprotein in serum.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (22 results)

All 2015 2014 2013 2012 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (8 results) (of which Invited: 2 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Decreased expression of innate immunity-related genes in peripheral blood mononuclear cells from patients with IgG4-related disease2015

    • Author(s)
      Akio Nakajima, Yasufumi Masaki, Takuji Nakamura, Takafumi Kawanami, Yasuhito Ishigaki, Tsutomu Takegami, Mitsuhiro Kawano, Kazunori Yamada, Norifumi Tsukamoto, Shoko Matsui, Takako Saeki, Kazuichi Okazaki, Terumi Kamisawa, Taiichiro Miyashita, Yoshihiro Yakushijin, Keita Fujikawa, Motohisa Yamamoto, et al.
    • Journal Title

      PLOS ONE

      Volume: -

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] <b>CD14</b><sup><b>+</b></sup><b> follicular dendritic cells in lymphoid follicles may play a role in the pathogenesis of IgG4-related </b><b>disease </b>2015

    • Author(s)
      Tomomi Satoh-Nakamura, Nozomu Kurose, Takafumi Kawanami, Takuji Nakamura, Haruka Iwao-Kawanami, Akio Nakajima, Miyuki Miki, Tomoyuki Sakai, Yoshimasa Fujita, Masao Tanaka, Toshihiro Fukushima, and Yasufumi Masaki
    • Journal Title

      Biomedical Research

      Volume: 36 Issue: 2 Pages: 143-153

    • DOI

      10.2220/biomedres.36.143

    • NAID

      130005065277

    • ISSN
      0388-6107, 1880-313X
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] IgG4-Related Disease: Diagnostic Methods and Therapeutic Strategies in Japan2014

    • Author(s)
      Yasufumi Masaki, Hironori Shimiz, Tomomi Sato Nakamura, Takuji Nakamura, Akio Nakajima, Haruka Iwao Kawanami, Miyuki Miki, Tomoyuki Sakai, Takafumi Kawanami, Yoshimasa Fujita, Masao Tanaka, and Toshiro Fukushima
    • Journal Title

      Journal of Clinical and Experimental Hematopathology

      Volume: 54 Pages: 95-101

    • NAID

      130004699344

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] IgG4-related disease and its pathogenesis-cross-talk between innate and acquired immunity.2014

    • Author(s)
      Umehara H, Nakajima A, Nakamura T, Kawanami T, Tanaka M, Dong L, Kawano M.
    • Journal Title

      Int Immunol.

      Volume: 26 Issue: 11 Pages: 585-95

    • DOI

      10.1093/intimm/dxu074

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Upregulation of aquaporin expression in the salivary glands of heat-acclimated rats2013

    • Author(s)
      Sugimoto N, Matsuzaki K, Ishibashi H, Tanaka M, Sawaki T, Fujita Y, Kawanami T, Masaki Y, Okazaki T, Sekine J, Koizumi S, Yachie A, Umehara H and Shido O
    • Journal Title

      Scientific Reports

      Volume: 3 Issue: 1 Pages: 1763-1763

    • DOI

      10.1038/srep01763

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Japanese variant of multicentirc cattleman's disease associated with serositis and thrombocytopenia-a report of two cases: Is TAFRO syndrome(Castleman-Kojima Disease) a distinct clinicopathological entity?2013

    • Author(s)
      Y.Masaki, A.Nakajima, H.Iwao, N.Kurose, T.Sato, T.Nakamura, M.Miki, T.Sakai, T.Kawanami, T.Sawaki, Y.Fujita, M.Tanaka, T.Fukushima, T.Okazaki, H.Umehara
    • Journal Title

      J. Clin. Exp. Hematop.

      Volume: 53 Pages: 57-61

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cutoff Values of Serum IgG4 and Histopathological IgG4+ Plasma Cells for Diagnosis of Patients with IgG4-Related Disease.2013

    • Author(s)
      Masaki Y, Kurose N, Yamamoto M, ,Yamada K, Kawano M, ,Eguchi K, Sugai S, Umehara H.
    • Journal Title

      Int J Rheumatol.

      Volume: Epub ahead of print Pages: 580814-580814

    • DOI

      10.1155/2012/580814

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Skewed production of IL-6 and TGFbeta by cultured salivary gland epithelial cells from patients with Sjogren's syndrome2012

    • Author(s)
      Kawanami T, Sawaki T, Sakai T, Miki M, Iwao H, Nakajima A, Nakamura T, Sato T, Fujita Y, Tanaka M, Masaki Y, Fukushima T, Hirose Y, Taniguchi M, Sugimoto N, Okazaki T and Umehara H
    • Journal Title

      PLoS One

      Volume: 7 Issue: 10 Pages: e45689-e45689

    • DOI

      10.1371/journal.pone.0045689

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Prevention of fasting-mediated bone marrow atrophy by leptin administration2012

    • Author(s)
      Fujita Y, Yanagida H, Mimori T, Jin ZX, Sakai T, Kawanami T, Sawaki T, Masaki Y, Fukushima T, Okazaki T and Umehara H
    • Journal Title

      Cellular immunology

      Volume: 273 Issue: 1 Pages: 52-58

    • DOI

      10.1016/j.cellimm.2011.11.007

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cellular heat acclimation regulates cell growth, cell morphology, mitogen-activated protein kinase activation, and expression of aquaporins in mouse fibroblast cells2012

    • Author(s)
      Sugimoto N, Shido O, Matsuzaki K, Ohno-Shosaku T, Hitomi Y, Tanaka M, Sawaki T, Fujita Y, Kawanami T, Masaki Y, Okazaki T, Nakamura H, Koizumi S, Yachie A and Umehara H
    • Journal Title

      Cell Physiol Biochem.

      Volume: 30 Issue: 2 Pages: 450-457

    • DOI

      10.1159/000339038

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] CD4+ T-cell dysfunctions through the impaired lipid rafts ameliorate concanavalin A-induced hepatitis in sphingomyelin synthase 1-knockout mice2012

    • Author(s)
      Dong L, Watanabe K, Itoh M, Huan CR, Tong XP, Nakamura T, Miki M, Iwao H, Nakajima A, Sakai T, Kawanami T, Sawaki T, Masaki Y, Fukushima T, Fujita Y, Tanaka M, Yano M, Okazaki T and Umehara H
    • Journal Title

      International immunology

      Volume: 24 Issue: 5 Pages: 327-337

    • DOI

      10.1093/intimm/dxs008

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] IgG4関連疾患患者の疾患特異的に変動する血清タンパク質・代謝物群の網羅解析2015

    • Author(s)
      河南 崇典
    • Organizer
      IgG4研究会
    • Place of Presentation
      福岡リーセントホテル(福岡県福岡市)
    • Year and Date
      2015-03-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] IgG4関連疾患患者の疾患特異的に変動する血清タンパク質・代謝物群の網羅解析2014

    • Author(s)
      河南 崇典
    • Organizer
      日本シェーグレン症候群学会学術集会
    • Place of Presentation
      ホテルニュー長崎(長崎県長崎市)
    • Year and Date
      2014-09-11 – 2014-09-14
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Proteomics analysis in IgG4-related disease2013

    • Author(s)
      T.Kawanami, T.Takata, H.Iwao, Y.Masaki, H.Shimizu, T.Sato, T.Nakamura, A.Nakajima, M.Miki, Y.Fujita, M.Tanaka, T.Fukushima, T.Okazaki, H.Umehara
    • Organizer
      12th International Symposium on Sj&#246;gren's Syndrome
    • Place of Presentation
      Kyoto Hotel Okura, 京都市
    • Related Report
      2013 Research-status Report
  • [Presentation] IgG4関連疾患の疾患特異的に変動するタンパク質群の網羅解析2013

    • Author(s)
      河南崇典, 高田尊信, 岩男 悠, 正木康史, 佐藤智美, 中村拓路, 中島章夫, 三木美由貴, 藤田義正, 田中真生, 福島俊洋, 岡崎俊朗, 梅原久範
    • Organizer
      第57回日本リウマチ学会総会・学術集会
    • Place of Presentation
      国立京都国際会館, 京都市
    • Related Report
      2013 Research-status Report
  • [Presentation] IgG4関連疾患の疾患特異的に変動するタンパク質群の網羅解析2013

    • Author(s)
      河南崇典, 梅原久範
    • Organizer
      第34回日本炎症・再生医学会
    • Place of Presentation
      国立京都国際会館, 京都市
    • Related Report
      2013 Research-status Report
  • [Presentation] IgG4関連疾患の疾患特異的に変動するタンパク質群の網羅解析2013

    • Author(s)
      河南崇典, 高田尊信, 岩男 悠, 正木康史, 清水啓智, 佐藤智美, 中村拓路, 中島章夫, 三木美由貴, 藤田義正, 田中真生, 福島俊洋, 岡崎俊朗, 梅原久範
    • Organizer
      第22回日本シェーグレン症候群学会学術集会
    • Place of Presentation
      ブリーゼプラザ小ホール7F, 大阪府大阪市北区
    • Related Report
      2013 Research-status Report
  • [Presentation] IgG4関連疾患の疾患特異的に 変動するタンパク質群の網羅解析2013

    • Author(s)
      河南崇典
    • Organizer
      日本リウマチ学会
    • Place of Presentation
      京都国際会館
    • Related Report
      2012 Research-status Report
  • [Presentation] IgG4関連疾患の疾患特異的に変動するタンパク質群の網羅解析2013

    • Author(s)
      河南崇典
    • Organizer
      日本炎症・再生医学会
    • Place of Presentation
      京都国際会館
    • Related Report
      2012 Research-status Report
  • [Book] 再生医療における臨床研究と製品開発2013

    • Author(s)
      河南崇典, 小川法良
    • Total Pages
      6
    • Publisher
      株式会社 技術情報協会
    • Related Report
      2013 Research-status Report
  • [Remarks] IgG4+MOLPS:Mikulicz病検討会

    • URL

      http://www.kanazawa-med.ac.jp/&#771;hematol/topics4.html

    • Related Report
      2013 Research-status Report
  • [Remarks] IgG4+MOLPS:Mikulicz病検討会

    • URL

      http://www.kanazawa-med.ac.jp/~hematol/topics4.html

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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