Proteomics analysis in IgG4-related disease

• Project/Area Number: 24791010
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Kanazawa Medical University
• Principal Investigator: KAWANAMI Takafumi 金沢医科大学, 医学部, 講師 (20350762)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: IgG4関連疾患 / プロテオミクス / クラススイッチ / Alpha-1 antitrypsin / LRA2G

Outline of Final Research Achievements

We conducted a comprehensive analysis of proteins that change in a disease specific manner, using the serum of patients with IgG4-related disease before and after treatment, and that of healthy individuals, in order to identify proteins that are associated with the pathogenesis of IgG4-related disease. After protein removal as a pretreatment, two-dimensional electrophoresis of the serum was conducted and silver staining method was used. We also cut out the spots which changed before and after treatment and detection was performed by ESI-MS/MS. In addition, we identified proteins whose expression significantly changed before and after treatment, as well as those between healthy individuals and patients and confirmed the results with ELISA.　From two IgG4-related disease cases, we extracted 38 proteins which significantly changed expression before and after treatment. We found the increase in inflammatory factors such as Alpha-1 antitrypsin, Leucin-rich alpha-2- glycoprotein in serum.

Research Products

• [Journal Article] Decreased expression of innate immunity-related genes in peripheral blood mononuclear cells from patients with IgG4-related disease (2015)
  • Author(s): Akio Nakajima, Yasufumi Masaki, Takuji Nakamura, Takafumi Kawanami, Yasuhito Ishigaki, Tsutomu Takegami, Mitsuhiro Kawano, Kazunori Yamada, Norifumi Tsukamoto, Shoko Matsui, Takako Saeki, Kazuichi Okazaki, Terumi Kamisawa, Taiichiro Miyashita, Yoshihiro Yakushijin, Keita Fujikawa, Motohisa Yamamoto, et al.
  • Journal Title: PLOS ONE Volume: -
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] <b>CD14</b>^<b>+</b><b> follicular dendritic cells in lymphoid follicles may play a role in the pathogenesis of IgG4-related </b><b>disease </b> (2015)
  • Author(s): Tomomi Satoh-Nakamura, Nozomu Kurose, Takafumi Kawanami, Takuji Nakamura, Haruka Iwao-Kawanami, Akio Nakajima, Miyuki Miki, Tomoyuki Sakai, Yoshimasa Fujita, Masao Tanaka, Toshihiro Fukushima, and Yasufumi Masaki
  • Journal Title: Biomedical Research Volume: 36 Issue: 2 Pages: 143-153
  • DOI: 10.2220/biomedres.36.143
  • NAID: 130005065277
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] IgG4-Related Disease: Diagnostic Methods and Therapeutic Strategies in Japan (2014)
  • Author(s): Yasufumi Masaki, Hironori Shimiz, Tomomi Sato Nakamura, Takuji Nakamura, Akio Nakajima, Haruka Iwao Kawanami, Miyuki Miki, Tomoyuki Sakai, Takafumi Kawanami, Yoshimasa Fujita, Masao Tanaka, and Toshiro Fukushima
  • Journal Title: Journal of Clinical and Experimental Hematopathology Volume: 54 Pages: 95-101
  • NAID: 130004699344
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] IgG4-related disease and its pathogenesis-cross-talk between innate and acquired immunity. (2014)
  • Author(s): Umehara H, Nakajima A, Nakamura T, Kawanami T, Tanaka M, Dong L, Kawano M.
  • Journal Title: Int Immunol. Volume: 26 Issue: 11 Pages: 585-95
  • DOI: 10.1093/intimm/dxu074
  • Peer Reviewed / Open Access
• [Journal Article] Upregulation of aquaporin expression in the salivary glands of heat-acclimated rats (2013)
  • Author(s): Sugimoto N, Matsuzaki K, Ishibashi H, Tanaka M, Sawaki T, Fujita Y, Kawanami T, Masaki Y, Okazaki T, Sekine J, Koizumi S, Yachie A, Umehara H and Shido O
  • Journal Title: Scientific Reports Volume: 3 Issue: 1 Pages: 1763-1763
  • DOI: 10.1038/srep01763
  • Peer Reviewed
• [Journal Article] Japanese variant of multicentirc cattleman's disease associated with serositis and thrombocytopenia-a report of two cases: Is TAFRO syndrome(Castleman-Kojima Disease) a distinct clinicopathological entity? (2013)
  • Author(s): Y.Masaki, A.Nakajima, H.Iwao, N.Kurose, T.Sato, T.Nakamura, M.Miki, T.Sakai, T.Kawanami, T.Sawaki, Y.Fujita, M.Tanaka, T.Fukushima, T.Okazaki, H.Umehara
  • Journal Title: J. Clin. Exp. Hematop. Volume: 53 Pages: 57-61
  • Peer Reviewed
• [Journal Article] Cutoff Values of Serum IgG4 and Histopathological IgG4+ Plasma Cells for Diagnosis of Patients with IgG4-Related Disease. (2013)
  • Author(s): Masaki Y, Kurose N, Yamamoto M, ,Yamada K, Kawano M, ,Eguchi K, Sugai S, Umehara H.
  • Journal Title: Int J Rheumatol. Volume: Epub ahead of print Pages: 580814-580814
  • DOI: 10.1155/2012/580814
  • Peer Reviewed
• [Journal Article] Skewed production of IL-6 and TGFbeta by cultured salivary gland epithelial cells from patients with Sjogren's syndrome (2012)
  • Author(s): Kawanami T, Sawaki T, Sakai T, Miki M, Iwao H, Nakajima A, Nakamura T, Sato T, Fujita Y, Tanaka M, Masaki Y, Fukushima T, Hirose Y, Taniguchi M, Sugimoto N, Okazaki T and Umehara H
  • Journal Title: PLoS One Volume: 7 Issue: 10 Pages: e45689-e45689
  • DOI: 10.1371/journal.pone.0045689
  • Peer Reviewed
• [Journal Article] Prevention of fasting-mediated bone marrow atrophy by leptin administration (2012)
  • Author(s): Fujita Y, Yanagida H, Mimori T, Jin ZX, Sakai T, Kawanami T, Sawaki T, Masaki Y, Fukushima T, Okazaki T and Umehara H
  • Journal Title: Cellular immunology Volume: 273 Issue: 1 Pages: 52-58
  • DOI: 10.1016/j.cellimm.2011.11.007
  • Peer Reviewed
• [Journal Article] Cellular heat acclimation regulates cell growth, cell morphology, mitogen-activated protein kinase activation, and expression of aquaporins in mouse fibroblast cells (2012)
  • Author(s): Sugimoto N, Shido O, Matsuzaki K, Ohno-Shosaku T, Hitomi Y, Tanaka M, Sawaki T, Fujita Y, Kawanami T, Masaki Y, Okazaki T, Nakamura H, Koizumi S, Yachie A and Umehara H
  • Journal Title: Cell Physiol Biochem. Volume: 30 Issue: 2 Pages: 450-457
  • DOI: 10.1159/000339038
  • Peer Reviewed
• [Journal Article] CD4+ T-cell dysfunctions through the impaired lipid rafts ameliorate concanavalin A-induced hepatitis in sphingomyelin synthase 1-knockout mice (2012)
  • Author(s): Dong L, Watanabe K, Itoh M, Huan CR, Tong XP, Nakamura T, Miki M, Iwao H, Nakajima A, Sakai T, Kawanami T, Sawaki T, Masaki Y, Fukushima T, Fujita Y, Tanaka M, Yano M, Okazaki T and Umehara H
  • Journal Title: International immunology Volume: 24 Issue: 5 Pages: 327-337
  • DOI: 10.1093/intimm/dxs008
  • Peer Reviewed
• [Presentation] IgG4関連疾患患者の疾患特異的に変動する血清タンパク質・代謝物群の網羅解析 (2015)
  • Author(s): 河南 崇典
  • Organizer: IgG4研究会
  • Place of Presentation: 福岡リーセントホテル（福岡県福岡市）
  • Year and Date: 2015-03-21
  • Invited
• [Presentation] IgG4関連疾患患者の疾患特異的に変動する血清タンパク質・代謝物群の網羅解析 (2014)
  • Author(s): 河南 崇典
  • Organizer: 日本シェーグレン症候群学会学術集会
  • Place of Presentation: ホテルニュー長崎（長崎県長崎市）
  • Year and Date: 2014-09-11 – 2014-09-14
  • Invited
• [Presentation] Proteomics analysis in IgG4-related disease (2013)
  • Author(s): T.Kawanami, T.Takata, H.Iwao, Y.Masaki, H.Shimizu, T.Sato, T.Nakamura, A.Nakajima, M.Miki, Y.Fujita, M.Tanaka, T.Fukushima, T.Okazaki, H.Umehara
  • Organizer: 12th International Symposium on Sj&#246;gren's Syndrome
  • Place of Presentation: Kyoto Hotel Okura, 京都市
• [Presentation] IgG4関連疾患の疾患特異的に変動するタンパク質群の網羅解析 (2013)
  • Author(s): 河南崇典, 高田尊信, 岩男 悠, 正木康史, 佐藤智美, 中村拓路, 中島章夫, 三木美由貴, 藤田義正, 田中真生, 福島俊洋, 岡崎俊朗, 梅原久範
  • Organizer: 第57回日本リウマチ学会総会・学術集会
  • Place of Presentation: 国立京都国際会館, 京都市
• [Presentation] IgG4関連疾患の疾患特異的に変動するタンパク質群の網羅解析 (2013)
  • Author(s): 河南崇典, 梅原久範
  • Organizer: 第34回日本炎症・再生医学会
  • Place of Presentation: 国立京都国際会館, 京都市
• [Presentation] IgG4関連疾患の疾患特異的に変動するタンパク質群の網羅解析 (2013)
  • Author(s): 河南崇典, 高田尊信, 岩男 悠, 正木康史, 清水啓智, 佐藤智美, 中村拓路, 中島章夫, 三木美由貴, 藤田義正, 田中真生, 福島俊洋, 岡崎俊朗, 梅原久範
  • Organizer: 第22回日本シェーグレン症候群学会学術集会
  • Place of Presentation: ブリーゼプラザ小ホール7F, 大阪府大阪市北区
• [Presentation] IgG4関連疾患の疾患特異的に 変動するタンパク質群の網羅解析 (2013)
  • Author(s): 河南崇典
  • Organizer: 日本リウマチ学会
  • Place of Presentation: 京都国際会館
• [Presentation] IgG4関連疾患の疾患特異的に変動するタンパク質群の網羅解析 (2013)
  • Author(s): 河南崇典
  • Organizer: 日本炎症・再生医学会
  • Place of Presentation: 京都国際会館
• [Book] 再生医療における臨床研究と製品開発 (2013)
  • Author(s): 河南崇典, 小川法良
  • Total Pages: 6
  • Publisher: 株式会社 技術情報協会
• [Remarks] IgG4+MOLPS：Mikulicz病検討会
  • URL: http://www.kanazawa-med.ac.jp/&#771;hematol/topics4.html
• [Remarks] IgG4+MOLPS：Mikulicz病検討会
  • URL: http://www.kanazawa-med.ac.jp/~hematol/topics4.html