Examination of anti-viral activity of novel phage-derived nucleosides
Project/Area Number |
24791025
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Infectious disease medicine
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Research Institution | Kochi University |
Principal Investigator |
UCHIYAMA Jumpei 高知大学, 教育研究部医療学系, 助教 (20574619)
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Research Collaborator |
GAMOH Keiji 高知大学, 教育研究部総合科学系, 教授 (90204817)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | ファージ / 感染症 / 核酸 / ウイルス / ヌクレオシド / LC-MS / 核酸分析 / bacteriophage / virus |
Outline of Final Research Achievements |
Nucleic acid analogs, which the structure of the nucleoside is based, is used in chemotherapy against viral infections. Some of the bacteriophages (phages) have unique genomic DNAs. In this study, we searched novel nucleic acids from phage genomic DNA, which can become drug discovery platform for anti-viral drugs. The phage genomic DNAs were analyzed using LC-MS. As a result, most of the phages were revealed to have normal genomic DNAs. On the other hand, Staphylococcus aureus phage S6 DNA was revealed to contain uracil in place of thymine as a nucleic acid base. Unfortunately, it did not lead to the discovery of novel nucleic acids that can be drug discovery platform for anti-viral drugs in the present study.
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Report
(4 results)
Research Products
(78 results)
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[Journal Article] Characterization of a novel Pseudomonas aeruginosa bacteriophage, KPP25, of the family Podoviridae2014
Author(s)
Miyata R, Yamaguchi K, Uchiyama J, Shigehisa R, Takemura-Uchiyama I, Kato S, Ujihara T, Sakaguchi Y, Daibata M, Matsuzaki S
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Journal Title
Virus Research
Volume: 189
Pages: 43-46
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] In silico analysis of AHJD-like viruses, Staphylococcus aureus phages S24-1 and S13', and study of phage S24-1 adsorption.2014
Author(s)
Uchiyama J, Takemura-Uchiyama I, Kato S, Sato M, Ujihara T, Matsui H, Hanaki H, Daibata M, Matsuzaki S.
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Journal Title
Microbiologyopen.
Volume: 3
Issue: 2
Pages: 257-270
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Draft Genome Sequence of Clostridium botulinum Type B Strain Osaka05, Isolated from an Infant Patient with Botulism in Japan.2014
Author(s)
Sakaguchi, Y., Hosomi, K., Uchiyama, J., Ogura, Y., Umeda, K., Sakaguchi, M., Kohda, T., Mukamoto, M., Misawa, N., Matsuzaki, S., Hayashi, T., Kozaki, S.
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Journal Title
Genome Announc.
Volume: 23
Issue: 1
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] バクテリオファージ療法の現状と可能性2014
Author(s)
松崎茂展,内山淳平,内山伊代,村上雅尚,大畑雅典
Organizer
2014年度日本農芸化学会西日本支部大会
Place of Presentation
佐賀県(HOTELグランデはがくれ)
Year and Date
2014-09-18 – 2014-09-19
Related Report
Invited
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[Presentation] KHP30-like phages among clinical isolates of Helicobacter pylori2014
Author(s)
6.Iyo Takemura-Uchiyama, Jumpei Uchiyama, Hiroaki Takeuchi, Keiji Gamoh, Yoshihiko Sakaguchi, Shin-ichiro Kato, Takako Ujihara, Masanori Daibata, Shigenobu Matsuzaki
Organizer
IUMS (International Union of Microbiological Societies Congress) 2014
Place of Presentation
Montreal, Canada(Montreal’s Convention Centre)
Year and Date
2014-07-27 – 2014-08-01
Related Report
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[Presentation] Analysis of botulinum neurotoxin type G gene-encoding plasmid in Clostridium argentinense strain 27402014
Author(s)
7.Yoshihiko Sakaguchi, Jumpei Uchiyama, Tomonori Suzuki, Yumiko Yamamoto, Koji Hosomi, Tomoko Kohda, Shigenobu Matsuzaki, Tetsuya Hayashi, Masafumi Mukamoto, Keiji Oguma
Organizer
IUMS (International Union of Microbiological Societies Congress) 2014
Place of Presentation
Montreal, Canada(Montreal’s Convention Centre)
Year and Date
2014-07-27 – 2014-08-01
Related Report
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[Presentation] Estimated distribution of KHP30-like bacteriophages in the stomach, using clinical Helicobacter pylori isolates2014
Author(s)
Jumpei Uchiyama, Hiroaki Takeuchi, Yoshihiko Sakaguchi, Iyo Takemura-Uchiyama, Shin-ichiro Kato, Keiji Gamoh, Takako Ujihara, Masanori Daibata, Shigenobu Matsuzaki
Organizer
The 12th Japan-Korea International Symposium on Microbiology (XII-JKISM)
Place of Presentation
Tokyo, Japan
Related Report
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[Presentation] Staphylococcus aureus CC398 increasingly causing invasive infections in humans: the consequence of the acquisition of a phage of bovine origin?2013
Author(s)
van der Mee-Marquet N, Girard M, Uchiyama J, Corvaglia A, Bertrand X, Kluytmans J, Donnio PY, Quentin R, Matsuzaki S, Francois P
Organizer
10th International Meeting on Microbial Epidemiological Markers (IMMEM-10)
Place of Presentation
Paris, France
Related Report
Invited
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[Presentation] A novel type of spherical bacteriophage, Helicobacter pylori bacteriophage KHP302013
Author(s)
Uchiyama J, Takeuchi H, Sakaguchi Y, Gamoh K, Kato S, Takemura-Uchiyama I, Ujihara T, Daibata M, Matsuzaki S
Organizer
The 5th Congress of European Microbiologists (FEMS 2013)
Place of Presentation
Leipzig, Germany
Related Report
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[Presentation] Analysis of botulinum neurotoxin type G gene-encoding plasmid in of Clostridium argentinense2013
Author(s)
Sakaguchi Y, Uchiyama J, Suzuki T, Yamamoto Y, Ogura Y, Sakaguchi M, Tsuda C, Matsuzaki S, Hayashi T, Oguma K
Organizer
The 5th Congress of European Microbiologists (FEMS 2013)
Place of Presentation
Leipzig, Germany
Related Report
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[Presentation] C型ボツリヌス毒素変換ファージのゲノム情報を基盤とした偽溶原性メカニズムの解析2013
Author(s)
阪口 義彦, Oliva Maria A., Martin-Galiano Antonio J., Andreu Jose M., 阪口 政清, 内山淳平, 小椋 義俊, 山本 由弥子, 鈴木 智典, 織田 華絵, 津田 千秋, 松崎 茂展, 林 哲也, 小熊 惠二
Organizer
第35回日本分子生物学会年会
Place of Presentation
福岡国際会議場・マリンメッセ福岡(福岡)
Related Report
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