The therapeutic research using Autophagy in Tuberous sclerosis complex

Research Project

Project/Area Number	24791074
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Pediatrics
Research Institution	Oita University
Principal Investigator	MIYAHARA HIROAKI 大分大学, 医学部, 講師 (00457615)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000) Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	結節性硬化症 / オートファジー / ウェスタンブロット / 細胞培養 / mTOR
Outline of Final Research Achievements	In this research, I have tried to elucidate the relationship between mTOR signaling pathway and autophagy in Tuberous sclerosis complex (TSC) and establish new therapeutic strategy using induction of autophagy. In western blotting, control fubroblast induced autophagy with starvation, but TSC fbroblast did not at all. p62, which is decrease with induction of autophagy, showed reduction tendency in both fibroblasts.