Identification of RPS6KA1 as novel molecular target of rhabdomyosarcoma

Research Project

Project/Area Number	24791078
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Pediatrics
Research Institution	Kyoto Prefectural University of Medicine
Principal Investigator	MIYACHI Mitsuru 京都府立医科大学, 医学(系)研究科(研究院), 助教 (40584983)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000) Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	RPS6KA1 / 横紋筋肉腫 / 分子標的療法
Research Abstract	We compared the expression pattern of mouse myoblasts which express rhabdomyosarcoma-specific fusion gene and identified the candidate gene as Rps6ka1. We examined rhabdomyosarcoma tumor specimens and found that RPS6KA1 is overexpressed compared with normal human skeletal muscle. RPS6KA1 inhibitor, BI-D1870, inhibited the proliferation of rhabdomyosarcoma cells and G2/M cell cycle arrest was observed. BI-D1870 treatment induced Myosin Heavy Chain expression and differentiation of rhabdomyosarcoma cells. RPS6KA1 may be a novel therapeutic target of rhabdomyosarcoma.