| Project/Area Number |
24791125
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Osaka Medical College |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Keywords | preterm infant / cerebellar injury / myelination / female hormones / progesterone / 子宮内炎症 / 早産児 / neurosteroid / allopregnanolone / 5α-reductase type2 / 小脳障害 / 女性ホルモン |
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| Outline of Final Research Achievements |
An intrauterine inflammation rat model was made by intraperitoneal injection of Lipopolysaccharide（LPS）to late gestational pregnant rats (LPS-treated group). The control rats were injected with the same volume of saline. After birth, the cerebellar tissues were harvested from 7 day-old rat pups of both groups. Real-time RT-PCR showed gene expression of myelinating markers, myelin basic protein (MBP) and 2’, 3’-cyclic nucleotide phosphodiesterase（CNP）both decreased significantly in the cerebellar tissues of LPS rats compared to those of control rats. Decreased cerebellar gene expression of MBP and CNP in LPS-treated rats could not be reversed by intrapelitoneal administration of progesterone to either mothers or neonates. We conclude that progesterone has no effect on myelination in the injured neonatal cerebellar white matter induced by intrauterine inflammation.
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