Immune regulation of mast cells in semaphorin 3a transgenic mouse

• Project/Area Number: 24791147
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: TATSUNO Kazuki 浜松医科大学, 医学部附属病院, 助教 (50436937)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: semaphorin / mast cell / SCF / proliferation / keratinocyte / 肥満細胞 / 細胞増殖 / stem cell factor / Semaphorin / Mast cell

Outline of Final Research Achievements

We developed a transgenic mice designed to over-express semaphoring 3a (Sema3a) in the basal layer of the epidermis. Increase in mast cell infiltration in the dermis of these mice was observed, and therefore, we investigated the interaction between sema3a and mast cells. We generated bone marrow derived mast cells from B6 mice, and analyzed the expression of sema3a receptor (receptor complex of NRP1 and Plexin A1). These cells showed expression of both molecules, and sema3a enhanced IL-3-induced mast cell proliferation. On the other hand, sema3a decreased the production of SCF from mouse keratinocytes, indicating the complex role of sema3a in the regulation of mast cell survival with in the skin.