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Intracellular tension-dependent regulation of regulation in human epidermal keratinocytes

Research Project

Project/Area Number 24791159
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Dermatology
Research InstitutionEhime University

Principal Investigator

NANBA Daisuke  愛媛大学, 上級研究員センター, 講師 (10380255)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords表皮角化幹細胞 / 角化幹細胞
Research Abstract

Understanding of the nature of human epidermal keratinocytes is significant for advances in therapies for disregulation of epidermal homeostasis and wound healing, and also in keratinocyte stem cell biology and regenerative medicine for burns. In this study, we revealed that the actin filaments was differently organized in keratinocyte stem/progenitor cells and transient amplifying (TA) cells, which was due to differetial activity of Rac1 proteins in each type of keratinocytes. Furthermore, Rac1 was essential for maintenance of human epidermal keratinocyte stem cells. We also found that cell motility which is associated with intracelluar tension was correlated with proliferation of human epidermal keratinocytes.These results indicate that cytoskeletal organization and intracellular tension are involved in proliferation of humam epidermal keratinocytes.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (23 results)

All 2013 2012 Other

All Journal Article (8 results) (of which Peer Reviewed: 4 results) Presentation (13 results) Remarks (2 results)

  • [Journal Article] Actin filament dynamics impacts keratinocyte stem cell maintenance2013

    • Author(s)
      Nanba D, Toki F, Matsushita N, Matsushita S, Higashiyama S, Barrandon Y
    • Journal Title

      EMBO Mol Med

      Volume: 5 Pages: 640-653

    • Related Report
      2013 Final Research Report
  • [Journal Article] Recent advances in the epidermal growth factor receptor/ligand biology on skin homeostasis and keratinocyte stem cell regulation2013

    • Author(s)
      Nanba D, Toki F, Barrandon Y, Higashiyama S
    • Journal Title

      J Dermatol Sci

      Volume: 72 Pages: 81-86

    • Related Report
      2013 Final Research Report
  • [Journal Article] Second harmonic generation reveals collagen fibril remodeling in fibroblast-populated collagen gels2013

    • Author(s)
      Toki F, Honkura N, Shirakata Y, Imamura T, Higashiyama S, Nanba D
    • Journal Title

      Cell Struct Funct

      Volume: 38 Pages: 227-236

    • NAID

      130004137598

    • Related Report
      2013 Final Research Report
  • [Journal Article] Efficient expansion of human keratinocyte stem / progenitor cells carrying a transgene with lentiviral vector2013

    • Author(s)
      Nanba D, Matsushita N, Toki F, 5.Higashiyama S
    • Journal Title

      Stem Cell Res Ther

      Volume: 4 Pages: 127-127

    • Related Report
      2013 Final Research Report
  • [Journal Article] Recent advances in the epidermal growth factor receptor/ligand system biology on skin homeostasis and keratinocyte stem cell regulation.2013

    • Author(s)
      Nanba D, Toki F, Higashiyama S, Barrandon Y.
    • Journal Title

      Journal of Dermatological Science

      Volume: 72 Issue: 2 Pages: 81-86

    • DOI

      10.1016/j.jdermsci.2013.05.009

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Second harmonic generation reveals collagen fibril remodeling in fibroblast-populated collagen gels.2013

    • Author(s)
      Toki F, Honkura N, Shirakata Y, Imamura T, Higashiyama S, Nanba D.
    • Journal Title

      Cell Structure andFunction

      Volume: 38 Pages: 227-236

    • NAID

      130004137598

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient expansion of huan keratinocyte stem/progenitor cells carrying a transgene with lentiviral vector.2013

    • Author(s)
      Nanba D, Matsushita N, Toki F, Higashiyama S.
    • Journal Title

      Stem Cell Research and Therapy

      Volume: 4 Issue: 5 Pages: 127-127

    • DOI

      10.1186/scrt338

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Actin filamet dynamics impacts keratinocyte stem cell maintenance2013

    • Author(s)
      Nanba D, Toki F, Matsushita N, Matsushita S, Higashiyama S, Barrandon Y
    • Journal Title

      EMBO Molecular Medicine

      Volume: 5 Issue: 4 Pages: 640-653

    • DOI

      10.1002/emmm.201201839

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] ヒト表皮角化幹細胞のダイナミクス2013

    • Author(s)
      難波大輔
    • Organizer
      第3回細胞再生医療研究会
    • Place of Presentation
      神戸
    • Year and Date
      2013-07-27
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Collective motion dynamics of human epidermal keratinocyte stem cells2013

    • Author(s)
      Nanba D, Toki F, Tate S, Imai M, Matsushita N, Toki H, Higashiyama S, Barrandon Y
    • Organizer
      Stem Cells in Translation
    • Place of Presentation
      Florence, Italy
    • Related Report
      2013 Final Research Report
  • [Presentation] A cell motion predicts keratinocyte stemness2013

    • Author(s)
      Nanba D, Toki F, Tate S, Imai M, Matsushita N, Toki H, Higashiyama S, Barrandon Y
    • Organizer
      The Physical Biology of Stem Cells
    • Place of Presentation
      Cambridge, UK
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト表皮角化幹細胞コロニー動態を集団動力学で読み解く2013

    • Author(s)
      難波大輔, 土岐富士緒, 松下夏樹, 田手壮太, 今井統, 土岐博, 東山繁樹, Yann Barrandon
    • Organizer
      日本再生医療学会第12回大会
    • Place of Presentation
      横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] A cell motion predicts keratinocyte stemness.2013

    • Author(s)
      Nanba D, Toki F, Tate S, Imai M, Matsushita N, Toki H, Higashiyama S, Barrandon Y.
    • Organizer
      The Physical Biology of Stem Cells
    • Place of Presentation
      Cambridge, UK
    • Related Report
      2013 Annual Research Report
  • [Presentation] Collective motion dynamics of human epidermal keratinocyte stem cells.2013

    • Author(s)
      Nanba D, Nanba F, Tate S, Imai M, Matsushita N, Toki H, Higashiyama S, Barrandon Y.
    • Organizer
      Stem Cells in Translation
    • Place of Presentation
      Florence, Italy
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヒト表皮角化幹細胞コロニー動態を集団動力学で読み解く2013

    • Author(s)
      難波大輔、土岐富士緒、松下夏樹、田手壮太、今井統、土岐博、東山繁樹、Yann Barrandon
    • Organizer
      日本再生医療学会 第12回大会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] Collective motion dynamics of human epidermal keratinocyte stem cells2012

    • Author(s)
      Nanba D, Toki F, Matsushita N, Toki H, Higashiyama S, Barrandon Y
    • Organizer
      日本研究皮膚科学会第37回大会
    • Place of Presentation
      那覇
    • Related Report
      2013 Final Research Report
  • [Presentation] Dynamics of cultured human epidermal keratinocyte stem cells. International Society of Stem Cell Research2012

    • Author(s)
      Nanba D, Toki F, Higashiyama S, Barrandon Y
    • Organizer
      10th Annual Meeting
    • Place of Presentation
      Yokohama
    • Related Report
      2013 Final Research Report
  • [Presentation] Dynamics of cultured human epidermal keratinocyte stem cells2012

    • Author(s)
      Nanba D, Toki F, Matsushita N, Toki H, Higashiyama S, Barrandon Y
    • Organizer
      日本発生生物学会第45回大会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] Dynamics of cultured human epidermal keratinocyte stem cells2012

    • Author(s)
      Nanba D, Toki F, Higashiyama S, Barrandon Y.
    • Organizer
      International Society of Stem Cell Research, 10th Annual Meeting
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] Collective motion dynamics of human epidermal keratinocyte stem cells2012

    • Author(s)
      Nanba D, Toki F, Matsushita N, Toki H, Higashiyama S, Barrandon Y
    • Organizer
      日本研究皮膚科学会 第37回大会
    • Place of Presentation
      那覇
    • Related Report
      2012 Research-status Report
  • [Presentation] Dynamics of cultured human epidermal keratinocyte stem cells2012

    • Author(s)
      Nanba D, Toki F, Matsushita N, Toki H, Higashiyama S, Barrandon Y
    • Organizer
      日本発生生物学会 第45回大会
    • Place of Presentation
      神戸
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.m.ehime-u.ac.jp/school/biochem2/

    • Related Report
      2013 Final Research Report
  • [Remarks] 愛媛大学プロテオサイエンスセンター細胞増殖・腫瘍制御部門

    • URL

      http://www.m.ehime-u.ac.jp/school/biochem2/

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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