Project/Area Number |
24791299
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Radiation science
|
Research Institution | Osaka University |
Principal Investigator |
SAITO Shigeyoshi 大阪大学, 医学(系)研究科(研究院), 助教 (40583068)
|
Project Period (FY) |
2012-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 胎生期被ばく / 放射線 / 生体イメージング / マイクロCT / 小動物MRI / 組織染色 / 胎生期放射線曝露 / 高磁場MRI / Micro-CT |
Research Abstract |
We assessed brain abnormalities in mice and rats exposed prenatally to radiation (X-rays) using magnetic resonance imaging (MRI), Micro-CT and histological experiments. These animals were exposed to different radiation doses (0.5, 1.0, and 1.5 Gy). Brain abnormalities were assessed in neonatal male mice and rats. In vivo/Ex vivo T2-weighted imaging and diffusion tensor imaging (DTI) were performed using 11.7 T MRI. Decreased brain volume, increased ventricle volume, and thinner cortices were observed in irradiated rats and mice using MRI. However, no abnormalities in the cortical 6-layered structure were observed in radiation-exposed mice and rats. T2W and DTI and histological experiments are useful tools for assessing cortical and hippocampal abnormalities after prenatal exposure to radiation in mice and rats.
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