Development of in-vivo imaging method for immune cell tracking in mouse brain by using magnetic resonance imaging
Project/Area Number |
24791302
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Radiation science
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Research Institution | Osaka University |
Principal Investigator |
MORI Yuki 大阪大学, 免疫学フロンティア研究センター, 特任助教(常勤) (10559355)
|
Project Period (FY) |
2012-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
Keywords | 磁気共鳴画像法 / 免疫細胞動態 / 細胞追跡 / 神経免疫学 |
Research Abstract |
We established highly sensitive in-vivo imaging techniques for sequential monitoring of cell migration in the mouse brain at the single-cell level by using 11.7 T high-field magnetic resonance imaging (MRI) scanner. MRI with intravenous administration of superparamagnetic particles of iron oxide (SPIO) can be used to successfully monitor the transmigration of peripheral phagocytes into healthy and inflamed brains, as intracellular absorbed-SPIO can pass through the blood-brain barrier. We successfully detected single cells in live animal brains by achieving images of high spatio-temporal resolution as a result of high SNR. In addition to the high magnetic field, improvements in MR techniques; such as the custom-made RF coil, optimization of MR parameters and optimization of contrast agents, were significant factors for this achievement.
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Report
(3 results)
Research Products
(35 results)