| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Research Abstract |
We established highly sensitive in-vivo imaging techniques for sequential monitoring of cell migration in the mouse brain at the single-cell level by using 11.7 T high-field magnetic resonance imaging (MRI) scanner. MRI with intravenous administration of superparamagnetic particles of iron oxide (SPIO) can be used to successfully monitor the transmigration of peripheral phagocytes into healthy and inflamed brains, as intracellular absorbed-SPIO can pass through the blood-brain barrier. We successfully detected single cells in live animal brains by achieving images of high spatio-temporal resolution as a result of high SNR. In addition to the high magnetic field, improvements in MR techniques; such as the custom-made RF coil, optimization of MR parameters and optimization of contrast agents, were significant factors for this achievement.
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