| Project/Area Number |
24791447
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Digestive surgery
|
|---|
| Research Institution | Keio University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | 癌幹細胞 / 食道癌 / cancer stem cell |
|---|
| Research Abstract |
Fibrinogen is one of the prognostic factors in esophageal cancer. In esophageal squamous carcinoma cell lines, integrin beta 3 (CD61/fibrinogen receptor) positive cell line (TE8) showed sphere-formation in serum-free medium containing growth factors (EGF and bFGF). This result suggested that CD61- positive cells have the property of self-renewal of cancer stem cell. Furthermore, the analysis of cell morphology functions, CD61-positive cells indicated the high chemotactic ability. On the other hand, to enrich for cancer stem cells, followed by purification of cancer stem cells by long-term exposure to anti-cancer agents (5FU) in esophageal squamous carcinoma cell lines TE10 and TE11. After six months, both cells have acquired the ability for drug resistance, and the expression variation of miRNAs (miR-200 family or miR-let-7 family) were detected.
|
