basic research for cerebral venous ischemia and endogenous neural stem cell

• Project/Area Number: 24791516
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cerebral neurosurgery
• Research Institution: Nara Medical University
• Principal Investigator: YASUHIRO Takeshima 奈良県立医科大学, 医学部, 助教 (60510203)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
• Keywords: 脳血管障害 / 脳静脈虚血 / 神経幹細胞 / 脳血流動態 / 内在性 / 脳虚血 / 静脈虚血 / 光凝固法

Outline of Final Research Achievements

Cerebral venous ischemia is a rare but potentially significant complication of neurosurgical procedures. However it is still unclear how cerebral venous occlusion affects regional endogenous neural stem cell and cerebral blood flow dynamics. To elucidate its pathophysiology in detail, we examined this study. Two cortical veins were occluded photochemically using rose Bengal dye in male Wistar rats. Histopathological examination of the anti-Nestin antibody was performed. In addition, regional cerebral blood flow was measured in real time with a laser speckle contrast imaging before and after venous occlusion. Immunohistochemical examination showed the expression of anti-Nestin antibody positive cells around the ischemic core. Laser speckle contrast imaging revealed a stepwise reduction in cerebral blood flow with significant differences. These results suggest that the endogenous neural stem cells may affect the trend of regional ischemic tissue during cerebral venous occlusions.

Research Products

• [Journal Article] Rheumatoid arthritis-induced lateral atlantoaxial subluxation with multiple vertebrobasilar infarctions. (2015)
  • Author(s): Takeshima Y, Matsuda R, Hironaka Y, Motoyama Y, Nakase H.
  • Journal Title: Spine (Phila Pa 1976 Volume: 40(3) Issue: 3 Pages: E186-E189
  • DOI: 10.1097/brs.0000000000000701
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cytotoxic human peripheral blood-derived γδT cells kill glioblastoma cell lines: implications for cell-based immunotherapy for patients with glioblastoma (2014)
  • Author(s): Nakazawa T, Nakamura M, Park YS, Motoyama Y, Hironaka Y, Nishimura F, Nakagawa I, Yamada S, Matsuda R, Tamura K, Sugimoto T, Takeshima Y, Marutani A, Tsujimura T, Ouji N, Ouji Y, Yoshikawa M, Nakase H
  • Journal Title: J Neurooncol Volume: 116 Issue: 1 Pages: 31-39
  • DOI: 10.1007/s11060-013-1258-4
  • Peer Reviewed
• [Journal Article] 頸椎変性疾患に対する頸椎椎弓形成術 (2014)
  • Author(s): 竹島靖浩、中瀬裕之
  • Journal Title: 脳神経外科速報 Volume: 24(4) Pages: 422-429
• [Journal Article] Early inhibition of natriuresis suppresses symptomatic cerebral vasospasm in patients with aneurysmal subarachnoid hemorrhage. (2013)
  • Author(s): Nakagawa I, Hironaka Y, Nishimura F, Takeshima Y, Matsuda R, Yamada S, Motoyama Y, Park YS, Nakase H.
  • Journal Title: Cerebrovasc Dis Volume: 35(2) Issue: 2 Pages: 131-7
  • DOI: 10.1159/000346586
  • Peer Reviewed
• [Journal Article] Post-ischemic Administration of Vascular Endothelial Growth Factor Inhibitor in a Rat Model of Cerebral Venous Infarction (2013)
  • Author(s): Miyake H, Nakagawa I, Takeshima Y, Nishimura F, Park YS, Nakamura M, Nakase H.
  • Journal Title: Neurologia medico-chirurgica Volume: 53 Issue: 3 Pages: 135-140
  • DOI: 10.2176/nmc.53.135
  • NAID: 10031138791
  • ISSN: 0470-8105, 1349-8029
  • Peer Reviewed
• [Journal Article] An unusual case of primary central nervous system germinoma with meningeal dissemination. (2012)
  • Author(s): Takeshima Y, Yamada S, Motoyama Y, Park YS, Nakase H.
  • Journal Title: Childs Nerv Syst Volume: 28(12) Issue: 12 Pages: 2173-6
  • DOI: 10.1007/s00381-012-1901-y
  • Peer Reviewed
• [Journal Article] Hemodialysis-related upper cervical extradural amyloidoma presenting with intractable radiculopathy (2012)
  • Author(s): Takeshima Y, Kotsugi M, Park YS, Nakase H.
  • Journal Title: Eur Spine J Volume: Suppl 4 Issue: S4 Pages: 463-466
  • DOI: 10.1007/s00586-011-2084-z
  • Peer Reviewed
• [Journal Article] Subarachnoid hemorrhage in a case of segmental arterial mediolysis with coexisting intracranial and intraabdominal aneurysms. (2012)
  • Author(s): Matsuda R, Hironaka Y, Takeshima Y, Park YS, Nakase H.
  • Journal Title: J Neurosurg Volume: 116(5) Issue: 5 Pages: 948-51
  • DOI: 10.3171/2012.1.jns111967
  • Peer Reviewed
• [Journal Article] 脊髄血管障害 (2012)
  • Author(s): 竹島靖浩、中瀬裕之
  • Journal Title: 医学と薬学 Volume: 68(2) Pages: 223-230
• [Presentation] ラット脳静脈も出るにおける局所脳血流動態の評価 (2015)
  • Author(s): 竹島靖浩、三宅 仁、中川一郎、中瀬裕之
  • Organizer: Stroke 2015 (第40回日本脳卒中学会総会)
  • Place of Presentation: 広島
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] Neuroprotection with Intraventricular Brain-Derived neurotrophic Factor in Rat Venous Occlusion Model
  • Author(s): 竹島 靖浩、中川 一郎、中瀬 裕之
  • Organizer: 日本循環代謝学会総会
  • Place of Presentation: ﾘｰｶﾞﾛｲﾔﾙﾎﾃﾙ広島(広島県)