The effectiveness of erythropoietin for discogenic low back pain

• Project/Area Number: 24791523
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Kitasato University
• Principal Investigator: INOUE Gen 北里大学, 医学部, 講師 (80594209)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: シュワン細胞 / 椎間板細胞 / 疼痛 / 椎間板

Research Abstract

The effectiveness of erythropoetin for rats discogenic low back pain was evaluated. The cells from nucleus pulpous were isolated and cultured, and supernatant of medium was obtained. This supernatant, which includes many kinds of cytokines and growth factors stimulates primary Schwann cells and the expression of MAP kinase were evaluated. Pretreatment with erythropoietin suppressed MAP kinase expression significantly. Also, in vivo, erythropoietin suppressed MAP kinase expression in dorsal root ganglion and glial activation in dorsal horn in spinal cord. Erythropoietin could be one of the effective treatment for discogenic low back pain.

Research Products

• [Journal Article] Evaluation of pain behavior and CGRP immunoreactive sensory nerve fibers in the spinal dorsal horn after sciatic nerve compression and application of nucleus pulposus in rats (2014)
  • Author(s): Kimura S, Sakuma Y, Suzuki M, Orita S, Yamauchi K, Inoue G, Aoki Y, Ishikawa T, Miyagi M, Kamoda H, Kubota G, Oikawa Y, Inage K, Sainoh T, Sato J, Nakamura J, Toyone T, Takahashi K, Ohtori S
  • Journal Title: Spine (Phila Pa 1976) Volume: 39 Issue: 6 Pages: 455-62
  • DOI: 10.1097/brs.0000000000000180
  • Peer Reviewed
• [Journal Article] Up-regulation of NaV1.7 in dorsal root ganglia after intervertebral disc injury in rats (2014)
  • Author(s): Sadamasu A, Sakuma Y, Suzuki M, Orita S, Yamauchi K, Inoue G, Aoki Y, Ishikawa T, Miyagi M, Kamoda H, Kubota G, Oikawa Y, Inage K, Sainoh T, Sato J, Nakamura J, Toyone T, Takahashi K, Ohtori S
  • Journal Title: Spine (Phila Pa 1976) Volume: 39 Issue: 7 Pages: E421-E426
  • DOI: 10.1097/brs.0000000000000229
  • Peer Reviewed
• [Journal Article] Dichotomizing sensory nerve fibers innervating both the lumbar vertebral body and the area surrounding the iliac crest: a possible mechanism of referred lateral back pain from lumbar vertebral body. (2013)
  • Author(s): Fujii T, Sakuma Y, Orita S, Inoue G, Ochiai N, Kuniyoshi K, Aoki Y, Ishikawa T, Miyagi M, Kamoda H, Suzuki M, Kubota G, Inage K, Sainoh T, Sato J, Yamauchi K, Toyone T, Nakamura J, Kishida S, Takahashi K, Koshi T, Ohtori S
  • Journal Title: Spine (Phila Pa 1976) Volume: 38 Issue: 25 Pages: 1459-65
  • DOI: 10.1097/brs.0b013e3182a879cd
  • Peer Reviewed
• [Journal Article] Assessment of pain behavior in a rat model of intervertebral disc injury using the CatWalk gait analysis system (2013)
  • Author(s): Miyagi M, Ishikawa T, Kamoda H, Suzuki M, Sakuma Y, Orita S, Oikawa Y, Aoki Y, Toyone T, Takahashi K, Inoue G, Ohtori S
  • Journal Title: Spine Volume: 38巻 Issue: 17 Pages: 1459-1465
  • DOI: 10.1097/brs.0b013e318299536a
  • Peer Reviewed
• [Journal Article] Comparison of CatWalk analysis and von Frey testing for pain assessment in a rat model of nerve crush plus inflammation (2013)
  • Author(s): Sakuma T, Kamoda H, Miyagi M, Ishikawa T, Arai G, Eguchi Y, Suzuki M, Oikawa Y, Sakuma Y, Kubota G, Inage K, Saino T, Orita S, Yamauchi K, Inoue G, Takahashi K, Ohtori S
  • Journal Title: Spine Volume: 38巻 Issue: 15 Pages: E919-E924
  • DOI: 10.1097/brs.0b013e318297bfb6
  • Peer Reviewed
• [Journal Article] In vivo transfection of proopiomelanocortin gene, precursor of endogenous endorphin, by radial shock waves alleviates neuropathic pain (2013)
  • Author(s): TETSUHIRO ISHIKAWA,MASAYUKI MIYAGI,MASAOMI YAMASHITA,HIROTO KAMODA,YAWARA EGUCHI,GEN ARAI,MIYAKO SAZUKI,YOSHIHIRO SAKUMA,YASUHIRO OIKAWA,SUMIHISA ORITA,GEN INOUE,TOMOYUKI OZAWA,YASUCHIKA AOKI,TOMOAKI TOYONE,KAZUHISA TAKAHASHI,ATSUSHI YAMAGUCHI,ＳＥＩＪＩ ＯＨＴＯＲＩ
  • Journal Title: Journal of Orthopedic Science Volume: Jul; 18(4) Issue: 4 Pages: 636-45
  • DOI: 10.1007/s00776-013-0397-y
  • Peer Reviewed
• [Journal Article] Assessment of pain behavior in a rat model of intervertebral disk injury using the CatWalk gait analysis system. (2013)
  • Author(s): Miyagi M, Ishikawa T, Kamoda H, Suzuki M, Sakuma Y, Orita S, Oikawa Y, Aoki Y, Toyone T, Takahashi K, Inoue G, Ohtori S.
  • Journal Title: Spine (Phila Pa 1976). Volume: May 3. [Epub ahead of print]
  • Peer Reviewed
• [Journal Article] Comparison of CatWalk analysis and von Frey testing for pain assessment in a rat model of nerve crush plus inflammation. (2013)
  • Author(s): Sakuma T, Kamoda H, Miyagi M, Ishikawa T, Arai G, Eguchi Y, Suzuki M, Oikawa Y, Sakuma Y, Kubota G, Inage K, Saino T, Orita S, Yamauchi K, Inoue G, Takahashi K, Ohtori S.
  • Journal Title: Spine (Phila Pa 1976). Volume: May 3. [Epub ahead of print]
  • Peer Reviewed
• [Journal Article] Quantitive measurement of rats myofascial low back pain using catWalk gait analysis system (2013)
  • Author(s): Inoue G, Ohtori S, Miyagi M, Ishikawa T, Takahashi K.
  • Journal Title: Spine (Phila Pa 1976). Volume: 未
  • Peer Reviewed
• [Journal Article] Acceleration of lumbar posterolateral fusion using a collagen-binding domain fused with bFGF and allografting in a rat model. (2013)
  • Author(s): Inoue G1, Uchida K1, Ueno M1, Saito W1, Nakazawa T1, Imura T, Yogoro M, Fujimaki H, Takahira N, Matsushita O, Takaso M.
  • Journal Title: Spine (Phila Pa 1976). Volume: 未
  • Peer Reviewed
• [Journal Article] ISSLS prize winner: disc dynamic compression in rats produces long-lasting increases in inflammatory mediators in discs and induces long-lasting nerve injury and regeneration of the afferent fibers innervating discs: a pathomechanism for chronic discogenic low back pain. (2012)
  • Author(s): Miyagi M, Ishikawa T, Kamoda H, Suzuki M, Murakami K, Shibayama M, Orita S, Eguchi Y, Arai G, Sakuma Y, Kubota G, Oikawa Y, Ozawa T, Aoki Y, Toyone T, Takahashi K, Inoue G, et al.
  • Journal Title: Spine (Phila Pa 1976). Volume: 37 Pages: 1810-1818
  • NAID: 10031169849
  • Peer Reviewed
• [Presentation] 慢性疼痛における塩基性線維芽細胞増殖因子の役割-痛みへの関与と治療への可能性 (2013)
  • Author(s): 井上玄, 内田健太郎, 上野正喜, 齋藤亘, 藤巻寿子, 中澤俊之, 井村貴之, 高平尚伸, 高相晶士
  • Organizer: 第28回日本整形外科学会基礎学術集会
  • Place of Presentation: 千葉市
  • Year and Date: 2013-10-18
• [Presentation] Quantitative measurement of rats myofascial low back pain using CatWalk gait analysis system (2013)
  • Author(s): Inoue G, Ohtori S, Miyagi M, Ishikawa T, Kamoda H, Eguchi Y, Suzuki M, Sakuma Y, Oikawa Y, Takaso M, Takahashi K
  • Organizer: The 14th EFFORT Congress
  • Place of Presentation: イスタンブール(トルコ)
  • Year and Date: 2013-06-05
• [Presentation] Quantitive measurement of rats myofascial low back pain using CatWalk gait analysis system (2013)
  • Author(s): Inoue G, Ohtori S, Miyagi M, Ishikawa T, Kamoda H, Eguchi Y, Suzuki M, Sakuma Y, Oikawa Y, Takaso M, Takahashi K
  • Organizer: The 14th EFORT Congress
  • Place of Presentation: Istanbul
• [Presentation] 末梢神経損傷モデルに対するエリスロポエチンの有用性に関する検討 (2013)
  • Author(s): 井上玄、Alban Gaultier、大鳥精司、高橋和久、W. Marie Campana
  • Organizer: 第42回日本脊椎脊髄病学会
  • Place of Presentation: 沖縄コンベンションセンター
• [Presentation] 慢性疼痛モデルにおける成長因子の役割に関する検討 (2013)
  • Author(s): 井上玄、内田健太郎、上野正喜、齋藤亘、中澤俊之、井村貴之、高平尚伸、高相晶士
  • Organizer: 第28回日本整形外科学会基礎学術集会
  • Place of Presentation: 幕張メッセ
  • Invited
• [Presentation] ラット骨粗鬆症モデル腰椎椎体骨髄内の感覚神経におけるカプサイシン受容体の動向 (2012)
  • Author(s): 鈴木都、吉野謙輔、井上玄、折田純久、宮城正行、石川哲大、鴨田博人、瓦井裕也、佐久間詳浩、及川泰宏、久保田剛、稲毛一秀、西能健、山内かづ代、高橋和久、大鳥精司
  • Organizer: 第5回日本運動器疼痛学会
  • Place of Presentation: ビッグサイトTFTホール(東京・有明)
• [Presentation] 痛み研究の最前線 慢性痛の分子メカニズム マウス神経障害性疼痛モデルでのマクロファージ、グリア活性に対するTNFαの関与 TNFαノックアウトマウスを用いた解析 (2012)
  • Author(s): 大鳥精司, 井上玄, 山内かづ代, 折田純久, 石川哲大, 鴨田博人, 鈴木都, 佐久間詳浩, 及川泰宏, 宮城正行, 高橋和久
  • Organizer: 第27回日本整形外科学会基礎学術集会
  • Place of Presentation: 名古屋国際会議場
  • Invited
• [Presentation] 機械的ストレスが骨粗鬆症性疼痛に及ぼす影響に関する検討 ラット骨粗鬆症尾椎圧迫モデルを用いた尾椎椎体および感覚神経の動態 (2012)
  • Author(s): 鈴木都, 井上玄, 大鳥精司, 折田純久, 宮城正行, 石川哲大, 鴨田博人, 佐久間詳浩, 及川泰宏, 山内かづ代, 高橋和久
  • Organizer: 第27回日本整形外科学会基礎学術集会
  • Place of Presentation: 名古屋国際会議場
• [Book] 上原記念生命科学財団研究報告集 (2012)
  • Author(s): 井上玄
  • Total Pages: 5
  • Publisher: 神経障害性疼痛における神経栄養因子に関する研究