| Project/Area Number |
24791540
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
TAKIMOTO Aki 京都大学, 健康長寿社会の総合医療開発ユニット, 特定助教 (00378902)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
|---|
| Keywords | 腱・靭帯 / コラーゲン線維 / コラーゲン線維形成 / 腱 / 靭帯 / コラーゲン / Scleraxis |
|---|
| Research Abstract |
Establishment of the musculoskeletal connections is critical for the formation of functional locomotive system. However, the molecular mechanism underlying the formation of entheses or musculo-tendinous junctions remains largely unknown. In this study, we generated mice lacking Hsp47, a collagen-specific molecular chaperon, and investigated how collagen fibrillogenesis in tendons affects the formation of the musculoskeletal connections. The tendon-specific Hsp47 conditional knockout mice exhibited drastic reduction of collagen fibers in tendons, while the cells expressing tendon-specific markers were still observed at the connection sites. Our results suggest that intercellular connections between tendon cells formed prior to the collagen fibrillogenesis play a crucial role in the establishment of musculoskeletal system during embryogenesis.
|
