The effect of micro RNA in bone remodering.

• Project/Area Number: 24791565
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Nippon Veterinary and Life Science University
• Principal Investigator: OCHI Hiroki 日本獣医生命科学大学, 獣医学部, 助教 (30582283)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 骨・軟骨代謝学 / マイクロRNA / 骨細胞 / 骨芽細胞 / 骨代謝 / miRNA

Research Abstract

In this study, we focused on the miRNA, and examined the effect of miRNA in osteblast and osteocyte differentiation. When miRNA was overexpressed in osteoblast, its differentiation was accelerated. Next, we identified the target genes of this miRNA by in silico analysis. The protein expression of these target genes were decreased by miRNA over-expression. When these target genes were knocked down by siRNA in osteoblast, osteoblast differentiation was poromorted. Moreover, the miRNA induced calcification of collagen sponge in mouse heterotopic ossification model. On the other hand, we isolated the osteocyte and osteoblast from osteocyte specific GFP expression mice by using the FACS. We performed new generation sequence to evaluate the expression of mRNA and miRNA in GFP positve cell (osteocyte). In osteocyte, we identified the some mRNA and miRNA which were higher or lower than osteoblast. We are progressing the evaluation of physiological function of these mRNA and miRNA in osteocyte.

Research Products

• [Journal Article] Hydroxyapatite/poly-L-lactide acid screws have better biocompatibility and femoral burr hole closure than does poly-L-lactide acid alone (2014)
  • Author(s): Akagi H, Iwata M, Ichinohe T, Amimoto H, Hayashi Y, Kannno N, Ochi H, Fujita Y, Harada Y, Tagawa M, Hara Y
  • Journal Title: J Biomater Appl Volume: 28(6) Issue: 6 Pages: 954-62
  • DOI: 10.1177/0885328213487754
• [Journal Article] Identification and Antimicrobial Susceptibility of Enterococci Isolated from Dogs and Cats Subjected to Differing Antibiotic Pressures (2013)
  • Author(s): Kataoka Y, Ito C, Sasaki A, Ishii M, Yamashiro S, Harada K, Ochi H, Sawada T
  • Journal Title: J Vet Med Sci Volume: 75(6) Pages: 749-53
  • NAID: 130003362112
• [Journal Article] Clinical efficacy of autogenous cancellous bone and fibroblast growth factor 2 combined with frozen allografts in femoral nonunion fractures (2013)
  • Author(s): Akagi H, Ochi H, Kannno N, Iwata M, Ichinohe T, Harada Y, Nezu Y, Yogo T, Tagawa M, Hara Y
  • Journal Title: Vet Comp Orthop Traumatol Volume: 15;26(2) Pages: 123-9
• [Journal Article] An analysis of skeletal development in osteoblast- and chondrocyte-specific Runx2 knockout mice (2013)
  • Author(s): Takeshi Takarada, Eiichi Hinoi, Ryota Nakazato, Hiroki Ochi, Cheng Xu, Azusa Tsuchikane, Shu Takeda, Gerard Karsenty, Takaya Abe, Hiroshi Kiyonari, and Yukio Yoneda
  • Journal Title: J. Bone Miner. Res Volume: 未定 Issue: 10 Pages: 2064-9
  • DOI: 10.1002/jbmr.1945
  • Peer Reviewed
• [Journal Article] Initial Responses of Articular Tissues in a Murine High-Fat Diet-Induced Osteoarthritis Model : Pivotal Role of the IPFP as a Cytokine Fountain (2013)
  • Author(s): Iwata M, Ochi H, Hara Y, Tagawa M, Koga D, Okawa A, Asou Y
  • Journal Title: PLoS One Volume: 12;8(4)(Print)
• [Journal Article] Variations in Gene and Protein Expression in Canine Chondrodystrophic Nucleus Pulposus Cells following Long-Term Three-Dimensional Culture. (2013)
  • Author(s): Iwata M, Ochi H, Asou Y, Haro H, Aikawa T, Harada Y, Nezu Y, Yogo T, Tagawa M, Hara Y.
  • Journal Title: PLoS One. Volume: 8 Issue: 5 Pages: e63120-e63120
  • DOI: 10.1371/journal.pone.0063120
  • Peer Reviewed
• [Journal Article] Sema3A regulates bone-mass accrual through sensory innervations (2013)
  • Author(s): Fukuda T, Takeda S, Xu R, Ochi H, Sunamura S, Sato T, Shibata S, Yoshida Y, Gu Z, Kimura A, Ma C, Xu C, Bando W, Fujita K, Shinomiya K, Hirai T, Asou Y, Enomoto M, Okano H, Okawa A, Itoh H
  • Journal Title: Nature. Volume: 497 Issue: 7450 Pages: 490-493
  • DOI: 10.1038/nature12115
  • Peer Reviewed
• [Journal Article] Sirt6 regulates postnatal growth plate differentiation and proliferation via Ihh signaling. (2013)
  • Author(s): Piao J, Tsuji K, Ochi H, Iwata M, Koga D, Okawa A, Morita S, Takeda S, Asou Y.
  • Journal Title: Ｓｃｉｅｎｔｉｆｉｃ Ｒｅｐｏｒｔ Volume: october 23 Issue: 1 Pages: 3022-3022
  • DOI: 10.1038/srep03022
  • Peer Reviewed
• [Journal Article] The role of individual domains and the significance of shedding of ATP6AP2/(pro)renin receptor in vacuolar H+-ATPase biogenesis (2013)
  • Author(s): Kinouchi et al
  • Journal Title: Plos One Volume: 8 Issue: 11 Pages: e78603-e78603
  • DOI: 10.1371/journal.pone.0078603
  • Peer Reviewed
• [Journal Article] Identification and Antimicrobial Susceptibility of Enterococci Isolated from Dogs and Cats Subjected to Differing Antibiotic Pressures. (2013)
  • Author(s): Kataoka Y, Ito C, Sasaki A, Ishii M, Yamashiro S, Harada K, Ochi H, Sawada T.
  • Journal Title: J Vet Med Sci. Volume: 75 Pages: 749-53
  • NAID: 130003362112
  • Peer Reviewed
• [Journal Article] Clinical efficacy of autogenous cancellous bone and fibroblast growth factor 2 combined with frozen allografts in femoral nonunion fractures. (2013)
  • Author(s): Akagi H, Ochi H, Kannno N, Iwata M, Ichinohe T, Harada Y, Nezu Y, Yogo T, Tagawa M, Hara Y.
  • Journal Title: Vet Comp Orthop Traumatol Volume: 26 Issue: 02 Pages: 123-9
  • DOI: 10.3415/vcot-11-12-0186
  • Peer Reviewed
• [Journal Article] Initial Responses of Articular Tissues in a Murine High-Fat Diet-Induced Osteoarthritis Model: Pivotal Role of the IPFP as a Cytokine Fountain. (2013)
  • Author(s): Iwata M, Ochi H, Hara Y, Tagawa M, Koga D, Okawa A, Asou Y.
  • Journal Title: PLoS One Volume: 12 Issue: 4 Pages: e60706-e60706
  • DOI: 10.1371/journal.pone.0060706
  • Peer Reviewed
• [Journal Article] Runx2 haploinsufficiency ameliorates the development of ossification of the posterior longitudinal ligament (2012)
  • Author(s): Iwasaki M, Piao J, Kimura A, Sato S, Inose H, Ochi H, Asou Y, Shinomiya K, Okawa A, Takeda S
  • Journal Title: PLoS One Volume: 7(8)
• [Journal Article] Procyanidin B3 prevents articular cartilage degeneration and heterotopic cartilage formation in a mouse surgical osteoarthritis model (2012)
  • Author(s): Aini H, Ochi H, Iwata M, Okawa A, Koga D, Okazaki M, Sano A, Asou Y
  • Journal Title: PLoS One Volume: 7(5)
• [Journal Article] Evaluation of the association between runt-related transcription factor 2 expression and intervertebral disk aging in dogs. (2012)
  • Author(s): Itoh H, Hara Y, Tagawa M, Kato T, Ochi H, Koga D, Okawa A, Asou Y.
  • Journal Title: Am J Vet Res. Volume: 73 Pages: 1553-1559
  • Peer Reviewed
• [Journal Article] Runx2 haploinsufficiency ameliorates the development of ossification of the posterior longitudinal ligament. (2012)
  • Author(s): Iwasaki M, Piao J, Kimura A, Sato S, Inose H, Ochi H, Asou Y, Shinomiya K, Okawa A, Takeda S.
  • Journal Title: PLoS One Volume: 7 Issue: 8 Pages: e43372-e43372
  • DOI: 10.1371/journal.pone.0043372
  • Peer Reviewed
• [Journal Article] Procyanidin B3 prevents articular cartilage degeneration and heterotopic cartilage formation in a mouse surgical osteoarthritis model. (2012)
  • Author(s): Aini H, Ochi H, Iwata M, Okawa A, Koga D, Okazaki M, Sano A, Asou Y.
  • Journal Title: PLoS One Volume: 7(5) Issue: 5 Pages: e37728-e37728
  • DOI: 10.1371/journal.pone.0037728
  • Peer Reviewed