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The effect of micro RNA in bone remodering.

Research Project

Project/Area Number 24791565
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthopaedic surgery
Research InstitutionNippon Veterinary and Life Science University

Principal Investigator

OCHI Hiroki  日本獣医生命科学大学, 獣医学部, 助教 (30582283)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords骨・軟骨代謝学 / マイクロRNA / 骨細胞 / 骨芽細胞 / 骨代謝 / miRNA
Research Abstract

In this study, we focused on the miRNA, and examined the effect of miRNA in osteblast and osteocyte differentiation. When miRNA was overexpressed in osteoblast, its differentiation was accelerated. Next, we identified the target genes of this miRNA by in silico analysis. The protein expression of these target genes were decreased by miRNA over-expression. When these target genes were knocked down by siRNA in osteoblast, osteoblast differentiation was poromorted. Moreover, the miRNA induced calcification of collagen sponge in mouse heterotopic ossification model. On the other hand, we isolated the osteocyte and osteoblast from osteocyte specific GFP expression mice by using the FACS. We performed new generation sequence to evaluate the expression of mRNA and miRNA in GFP positve cell (osteocyte). In osteocyte, we identified the some mRNA and miRNA which were higher or lower than osteoblast. We are progressing the evaluation of physiological function of these mRNA and miRNA in osteocyte.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (17 results)

All 2014 2013 2012

All Journal Article (17 results) (of which Peer Reviewed: 11 results)

  • [Journal Article] Hydroxyapatite/poly-L-lactide acid screws have better biocompatibility and femoral burr hole closure than does poly-L-lactide acid alone2014

    • Author(s)
      Akagi H, Iwata M, Ichinohe T, Amimoto H, Hayashi Y, Kannno N, Ochi H, Fujita Y, Harada Y, Tagawa M, Hara Y
    • Journal Title

      J Biomater Appl

      Volume: 28(6) Issue: 6 Pages: 954-62

    • DOI

      10.1177/0885328213487754

    • Related Report
      2013 Final Research Report
  • [Journal Article] Identification and Antimicrobial Susceptibility of Enterococci Isolated from Dogs and Cats Subjected to Differing Antibiotic Pressures2013

    • Author(s)
      Kataoka Y, Ito C, Sasaki A, Ishii M, Yamashiro S, Harada K, Ochi H, Sawada T
    • Journal Title

      J Vet Med Sci

      Volume: 75(6) Pages: 749-53

    • NAID

      130003362112

    • Related Report
      2013 Final Research Report
  • [Journal Article] Clinical efficacy of autogenous cancellous bone and fibroblast growth factor 2 combined with frozen allografts in femoral nonunion fractures2013

    • Author(s)
      Akagi H, Ochi H, Kannno N, Iwata M, Ichinohe T, Harada Y, Nezu Y, Yogo T, Tagawa M, Hara Y
    • Journal Title

      Vet Comp Orthop Traumatol

      Volume: 15;26(2) Pages: 123-9

    • Related Report
      2013 Final Research Report
  • [Journal Article] An analysis of skeletal development in osteoblast- and chondrocyte-specific Runx2 knockout mice2013

    • Author(s)
      Takeshi Takarada, Eiichi Hinoi, Ryota Nakazato, Hiroki Ochi, Cheng Xu, Azusa Tsuchikane, Shu Takeda, Gerard Karsenty, Takaya Abe, Hiroshi Kiyonari, and Yukio Yoneda
    • Journal Title

      J. Bone Miner. Res

      Volume: 未定 Issue: 10 Pages: 2064-9

    • DOI

      10.1002/jbmr.1945

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Initial Responses of Articular Tissues in a Murine High-Fat Diet-Induced Osteoarthritis Model : Pivotal Role of the IPFP as a Cytokine Fountain2013

    • Author(s)
      Iwata M, Ochi H, Hara Y, Tagawa M, Koga D, Okawa A, Asou Y
    • Journal Title

      PLoS One

      Volume: 12;8(4)(Print)

    • Related Report
      2013 Final Research Report
  • [Journal Article] Variations in Gene and Protein Expression in Canine Chondrodystrophic Nucleus Pulposus Cells following Long-Term Three-Dimensional Culture.2013

    • Author(s)
      Iwata M, Ochi H, Asou Y, Haro H, Aikawa T, Harada Y, Nezu Y, Yogo T, Tagawa M, Hara Y.
    • Journal Title

      PLoS One.

      Volume: 8 Issue: 5 Pages: e63120-e63120

    • DOI

      10.1371/journal.pone.0063120

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sema3A regulates bone-mass accrual through sensory innervations2013

    • Author(s)
      Fukuda T, Takeda S, Xu R, Ochi H, Sunamura S, Sato T, Shibata S, Yoshida Y, Gu Z, Kimura A, Ma C, Xu C, Bando W, Fujita K, Shinomiya K, Hirai T, Asou Y, Enomoto M, Okano H, Okawa A, Itoh H
    • Journal Title

      Nature.

      Volume: 497 Issue: 7450 Pages: 490-493

    • DOI

      10.1038/nature12115

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sirt6 regulates postnatal growth plate differentiation and proliferation via Ihh signaling.2013

    • Author(s)
      Piao J, Tsuji K, Ochi H, Iwata M, Koga D, Okawa A, Morita S, Takeda S, Asou Y.
    • Journal Title

      Scientific Report

      Volume: october 23 Issue: 1 Pages: 3022-3022

    • DOI

      10.1038/srep03022

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The role of individual domains and the significance of shedding of ATP6AP2/(pro)renin receptor in vacuolar H+-ATPase biogenesis2013

    • Author(s)
      Kinouchi et al
    • Journal Title

      Plos One

      Volume: 8 Issue: 11 Pages: e78603-e78603

    • DOI

      10.1371/journal.pone.0078603

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification and Antimicrobial Susceptibility of Enterococci Isolated from Dogs and Cats Subjected to Differing Antibiotic Pressures.2013

    • Author(s)
      Kataoka Y, Ito C, Sasaki A, Ishii M, Yamashiro S, Harada K, Ochi H, Sawada T.
    • Journal Title

      J Vet Med Sci.

      Volume: 75 Pages: 749-53

    • NAID

      130003362112

    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Clinical efficacy of autogenous cancellous bone and fibroblast growth factor 2 combined with frozen allografts in femoral nonunion fractures.2013

    • Author(s)
      Akagi H, Ochi H, Kannno N, Iwata M, Ichinohe T, Harada Y, Nezu Y, Yogo T, Tagawa M, Hara Y.
    • Journal Title

      Vet Comp Orthop Traumatol

      Volume: 26 Issue: 02 Pages: 123-9

    • DOI

      10.3415/vcot-11-12-0186

    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Initial Responses of Articular Tissues in a Murine High-Fat Diet-Induced Osteoarthritis Model: Pivotal Role of the IPFP as a Cytokine Fountain.2013

    • Author(s)
      Iwata M, Ochi H, Hara Y, Tagawa M, Koga D, Okawa A, Asou Y.
    • Journal Title

      PLoS One

      Volume: 12 Issue: 4 Pages: e60706-e60706

    • DOI

      10.1371/journal.pone.0060706

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Runx2 haploinsufficiency ameliorates the development of ossification of the posterior longitudinal ligament2012

    • Author(s)
      Iwasaki M, Piao J, Kimura A, Sato S, Inose H, Ochi H, Asou Y, Shinomiya K, Okawa A, Takeda S
    • Journal Title

      PLoS One

      Volume: 7(8)

    • Related Report
      2013 Final Research Report
  • [Journal Article] Procyanidin B3 prevents articular cartilage degeneration and heterotopic cartilage formation in a mouse surgical osteoarthritis model2012

    • Author(s)
      Aini H, Ochi H, Iwata M, Okawa A, Koga D, Okazaki M, Sano A, Asou Y
    • Journal Title

      PLoS One

      Volume: 7(5)

    • Related Report
      2013 Final Research Report
  • [Journal Article] Evaluation of the association between runt-related transcription factor 2 expression and intervertebral disk aging in dogs.2012

    • Author(s)
      Itoh H, Hara Y, Tagawa M, Kato T, Ochi H, Koga D, Okawa A, Asou Y.
    • Journal Title

      Am J Vet Res.

      Volume: 73 Pages: 1553-1559

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Runx2 haploinsufficiency ameliorates the development of ossification of the posterior longitudinal ligament.2012

    • Author(s)
      Iwasaki M, Piao J, Kimura A, Sato S, Inose H, Ochi H, Asou Y, Shinomiya K, Okawa A, Takeda S.
    • Journal Title

      PLoS One

      Volume: 7 Issue: 8 Pages: e43372-e43372

    • DOI

      10.1371/journal.pone.0043372

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Procyanidin B3 prevents articular cartilage degeneration and heterotopic cartilage formation in a mouse surgical osteoarthritis model.2012

    • Author(s)
      Aini H, Ochi H, Iwata M, Okawa A, Koga D, Okazaki M, Sano A, Asou Y.
    • Journal Title

      PLoS One

      Volume: 7(5) Issue: 5 Pages: e37728-e37728

    • DOI

      10.1371/journal.pone.0037728

    • Related Report
      2012 Research-status Report
    • Peer Reviewed

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Published: 2013-05-31   Modified: 2019-07-29  

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