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Construction of biomarker in urological cancer targeting the O-glycan

Research Project

Project/Area Number 24791631
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Urology
Research InstitutionHirosaki University

Principal Investigator

Tobisawa Yuki  弘前大学, 医学(系)研究科(研究院), 助教 (70623768)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Keywords糖転移酵素 / 糖鎖生物学 / バイオマーカー / 前立腺癌 / 糖鎖抗原 / モノクローナル抗体 / 癌 / マーカー
Outline of Final Research Achievements

Core2 β-1,6-N-acetylglucosaminyltransferase-1 (GCNT1) is a key enzyme that forms core 2-branched O-glycans.Paraffin-embedded PCa specimens were analyzed by immunohistochemistry for GCNT1 expression. GCNT1-negative tumors were associated with significantly better prostate-specific antigen (PSA)-free survival compared with GCNT1-positive tumors. Multivariate analysis revealed that detection of GCNT1 expression was an independent risk factor for PSA recurrence. We established new methods for GCNT1 detection from PCa specimens. Immunoblotting was used to examine post-digital rectal examination (DRE) urine from PCa patients. Over 90% of GCNT1-positive PCa patients with high concentrations of PSA showed extracapsular extension. In conclusion, GCNT1 expression closely associates with the aggressive potential of PCa. Further research aims to develop GCNT1 detection in post-DRE urine as a marker for PCa aggressiveness.

Report

(4 results)
  • 2015 Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (20 results)

All 2015 2014 2013 2012

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 2 results,  Open Access: 2 results) Presentation (14 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Prostate-specific antigen density predicts extracapsular extension and increased risk of biochemical recurrence in patients with high-risk prostate cancer who underwent radical prostatectomy.2015

    • Author(s)
      Koie T, Mitsuzuka K, Yoneyama T, Narita S, Kawamura S, Kaiho Y, Tsuchiya N, Tochigi T, Habuchi T, Arai Y, Ohyama C, Yoneyama T, Tobisawa Y.
    • Journal Title

      Int J Clin Oncol.

      Volume: 20(1) Issue: 1 Pages: 176-81

    • DOI

      10.1007/s10147-014-0696-0

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Neoadjuvant gemcitabine and carboplatin followed by immediate cystectomy may be associated with a survival benefit in patients with clinical T2 bladder cancer.2014

    • Author(s)
      Koie T, Ohyama C, Yamamoto H, Imai A, Hatakeyama S, Yoneyama T, Hashimoto Y, Yoneyama T, Tobisawa Y.
    • Journal Title

      Med Oncol.

      Volume: 31 Issue: 5 Pages: 949-949

    • DOI

      10.1007/s12032-014-0949-9

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Measurement of aberrant glycosylation of prostate specific antigen can improve specificity in early detection of prostate cancer.2014

    • Author(s)
      Yoneyama T, Ohyama C, Hatakeyama S, Narita S, Habuchi T, Koie T, Mori K, Hidari KI, Yamaguchi M, Suzuki T, Tobisawa Y
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 448 Issue: 4 Pages: 390-396

    • DOI

      10.1016/j.bbrc.2014.04.107

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Serum tri-and tetra-antennary N-glycan is a potential predictive biomarker for castration-resistant prostate cancer.2014

    • Author(s)
      Ishibashi Y, Tobisawa Y, Hatakeyama S, Ohashi T, Tanaka M, Narita S, Koie T, Habuchi T, Nishimura S, Ohyama C, Yoneyama T
    • Journal Title

      Prostate

      Volume: 74 Issue: 15 Pages: 1521-1529

    • DOI

      10.1002/pros.22869

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Neoadjuvant luteinizing-hormone-releasing hormone agonist plus low-dose estramustine phosphate improves prostate-specific antigen-free survival in high-risk prostate cancer patients: a propensity score-matched analysis.2014

    • Author(s)
      Koie T, Mitsuzuka K, Yoneyama T, Narita S, Kawamura S, Kaiho Y, Tsuchiya N, Tochigi T, Habuchi T, Arai Y, Ohyama C, Yoneyama T, Tobisawa Y.
    • Journal Title

      Int J Clin Oncol

      Volume: not yet

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Core2 β-1, 6-N-acetylglucosaminyltransferaseに対する新規モノクローナル抗体の樹立と本酵素による前立腺癌の悪性度増強機構の解明.2014

    • Author(s)
      飛澤悠葵,米山 徹,畠山真吾,溝上 敦,大山 力
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 前立腺マッサージ尿中のcore2 beta-1,6-N-acetylglucosaminyltransferaseの発現はpT3予測に有用である2014

    • Author(s)
      飛澤悠葵,米山 徹,畠山真吾,岡本哲平,山本勇人,今井 篤,米山高弘,橋本安弘,古家琢也,大山 力
    • Organizer
      第79回日本泌尿器科学会東部総会
    • Place of Presentation
      横浜
    • Year and Date
      2014-10-12 – 2014-10-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] pT3およびPSA再発を術前に予測する新規前立腺癌悪性度診断マーカーの開発2014

    • Author(s)
      飛澤悠葵,米山 徹,畠山真吾,岡本哲平,杉山尚樹,鈴木裕一郎,岡本亜希子,山本勇人,今井 篤,米山高弘,橋本安弘,古家琢也,大山 力
    • Organizer
      第102回日本泌尿器科学会総会
    • Place of Presentation
      神戸
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Detection of core2 beta-1,6-N-acetylglucosaminyltransferase in post-digital rectal examination urine is an independent predictor for extraprostatic extension of prostate cancer2014

    • Author(s)
      Tobisawa Y, Yoneyama T, Hatakeyama S, Hashimoto Y, Koie T, Ohyama C.
    • Organizer
      AUA annual meeting 2014
    • Place of Presentation
      Orland, FL, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] Glycan modification of surface glycoproteins on prostate cancer induces secretion of growth factors via enhanced interaction with prostate stromal cells2014

    • Author(s)
      Tobisawa Y, Yoneyama T, Hatakeyama S, Hashimoto Y, Koie T, Ohyama C.
    • Organizer
      EAU 29th Annual Congress
    • Place of Presentation
      Stockholm, Sweden
    • Related Report
      2013 Research-status Report
  • [Presentation] Immunohistochemical detection of core2 beta-1,6-N-acetylglucosaminyltransferase is an independent risk factor for PSA recurrence after radical prostatectomy2014

    • Author(s)
      Tobisawa Y, Yoneyama T, Hatakeyama S, Hashimoto Y, Koie T, Ohyama C.
    • Organizer
      EAU 29th Annual Congress
    • Place of Presentation
      Stockholm, Sweden
    • Related Report
      2013 Research-status Report
  • [Presentation] 前立腺外への浸潤を術前に予測する新規前立腺癌悪性度診断マーカーの開発2014

    • Author(s)
      飛澤悠葵、米山 徹、畠山真吾、橋本安弘、古家琢也、大山 力
    • Organizer
      第 23回泌尿器科分子・細胞研究会
    • Place of Presentation
      山形
    • Related Report
      2013 Research-status Report
  • [Presentation] 糖転移酵素C2GnTは前立腺癌と間質細胞との相互作用を高め前立腺癌の増殖を制御する2013

    • Author(s)
      飛澤悠葵, 米山 徹, 盛 和行, 畠山真吾, 今井 篤, 岡本亜希子, 山本勇人, 杉山尚樹, 岡本哲平, 鈴木裕一朗, 萩原和久, 及川真亮, 成田拓磨, 米山高弘, 橋本安弘,古家琢也, 大山 力
    • Organizer
      第22回泌尿器科分子・細胞研究会
    • Place of Presentation
      高知
    • Related Report
      2012 Research-status Report
  • [Presentation] Core2 O-glycan 陽性ポドプラニンの筋層湿潤膀胱癌における役割2012

    • Author(s)
      飛澤悠葵,岡本哲平,畠山真吾,米山 徹,大山 力
    • Organizer
      第6回GFRG研究会
    • Place of Presentation
      品川区
    • Related Report
      2012 Research-status Report
  • [Presentation] 前立腺全摘標本における糖転移酵素 core 2 β-1、6-N- acetylglucosaminylttransferase の免疫組織化学的評価はPSA再発予知法として有用である2012

    • Author(s)
      飛澤悠葵,米山 徹,畠山真吾,山本勇人,岡本哲平,鈴木裕一朗,米山高弘,古家琢也,岩村大径,佐藤天童,小島由太,神村典孝,大山 力
    • Organizer
      第77回日本泌尿器科学会東部総会
    • Place of Presentation
      文京区
    • Related Report
      2012 Research-status Report
  • [Presentation] 筋層湿潤膀胱癌に発現するムチン様タンパク質ポドプラニンにおけるコア2糖鎖構造の役割2012

    • Author(s)
      飛澤悠葵,岡本哲平,畠山真吾,米山 徹,大山 力
    • Organizer
      第6回東北糖鎖研究会
    • Place of Presentation
      弘前市
    • Related Report
      2012 Research-status Report
  • [Presentation] C2GnTは前立腺癌の悪性度マーカーになりうる2012

    • Author(s)
      飛澤悠葵
    • Organizer
      第8回臨床糖鎖研究会
    • Place of Presentation
      弘前市
    • Related Report
      2012 Research-status Report
  • [Presentation] Expression of core 2 β - 1, 6 - N - acetylglucosaminyltransferase is an independent risk factor for PSA recurrence after radical prostatectomy and closely associated with aggressive potential2012

    • Author(s)
      Tobisawa Y, Yoneyama T, Hatakeyama S, Yamamoto H, Koie T, Yoneyama T, Okamoto T, Kamimura N, Ohyama C
    • Organizer
      Glycobiology Meeting 2012
    • Place of Presentation
      サンディエゴ
    • Related Report
      2012 Research-status Report
  • [Presentation] Expression of core 2 beta-1, 6-N-acetylglucosaminyltransferase correlate with tumor progression in prostate cancer2012

    • Author(s)
      Tobisawa Y, Hatakeyama S, Yoneyama T, Koie T, Yoneyama T, Sato T, Fujita N, Ohyama C
    • Organizer
      第29回日韓泌尿器科会議
    • Place of Presentation
      鹿児島市
    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] 抗コア2β1,6-N-アセチルグルコサミニルトランスフェラーゼIモノクローナル抗体を利用して癌の悪性度を診断する方法2012

    • Inventor(s)
      大山 力、米山 徹、飛澤悠葵、畠山真吾
    • Industrial Property Rights Holder
      大山 力、米山 徹、飛澤悠葵、畠山真吾
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2012-232564
    • Filing Date
      2012-10-22
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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