Identification of characteristic gene expression and molecular target in sarcomatoid renal cell carcinoma

• Project/Area Number: 24791634
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: University of Tsukuba
• Principal Investigator: KOJIMA Takahiro 筑波大学, 医学医療系, 講師 (40626892)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 肉腫様腎細胞癌 / 遺伝子異常 / DNAコピー数異常 / マイクロアレイ

Research Abstract

We selected and analyzed ETV5, TOP2, RAC and VAV1 for candidate targets among up-regulated genes in sarcomatoid renal cell carcinoma. These genes were significantly up-regulated in sarcomatoid component compared with clear cell component. Suppression of these genes by siRNA resulted in suppression of cell proliferation of renal cell carcinoma cell lines. Our results may represent a novel biomarker and therapeutic target for patients with sarcomatoid renal cell carcinoma.

Research Products

• [Journal Article] Oncological outcomes of metastatic testicular cancers under centralized management through regional medical network (2013)
  • Author(s): Inai H, Kawai K, Kojima T, Joraku A, Shimazui T, Yamauchi A, Miyagawa T, Endo T, Fukuhara Y, Miyazaki J, Uchida K, Nishiyama H
  • Journal Title: Jpn J Clin Oncol Volume: 43(12) Issue: 12 Pages: 1249-54
  • DOI: 10.1093/jjco/hyt152
  • Peer Reviewed
• [Journal Article] Case of metastatic malignant melanoma of the urinary bladder (2013)
  • Author(s): Ikeda A, Miyagawa T, Kurobe M, Uchida M, Kojima T, Tsutsumi M, Ito S, Sugita S, Nishiyama H
  • Journal Title: Hinyokika Kiyo Volume: 59(9) Pages: 579-82
  • NAID: 120005333767
  • Peer Reviewed
• [Journal Article] Systemic transduction of p16INK4A antitumor peptide inhibits the growth of MBT-2 mouse bladder tumor cell line grafts (2013)
  • Author(s): Shimazui T, Yoshikawa K, Miyazaki J, Kojima T, Inai H, Ando S, Uemura H, Uchida K, Nishiyama H
  • Journal Title: Int. J. Oncol Volume: Vo l .42 Issue: 2 Pages: 543-54
  • DOI: 10.3892/ijo.2012.1752
  • Peer Reviewed
• [Journal Article] Delayed cutaneous wound healing in Fam129b/Minerva-deficient mice. (2012)
  • Author(s): Oishi H et al.
  • Journal Title: J. Biochem Volume: 152 Issue: 6 Pages: 549-555
  • DOI: 10.1093/jb/mvs100
  • Peer Reviewed
• [Journal Article] Management of ureteral obstruction in advanced testicular tumor with lymph node metastasis (2012)
  • Author(s): Ikeda A, Kawai K, Miyazaki J, Nishiyama H.
  • Journal Title: Jpn J Clin Oncol Volume: 42(8) Issue: 8 Pages: 52-748
  • DOI: 10.1093/jjco/hys094
  • NAID: 120007137102
  • Peer Reviewed
• [Journal Article] A case of acute adrenal insufficiency unmasked during sunitinib treatment for metastatic renal cell carcinoma (2012)
  • Author(s): Yoshino T, Kawai K, Miyazaki J, Kimura T, Ikeda A, Takaoka E, Suetomi T, Oikawa T, Kojima T, Iwasaki H, Shimano H, Nishiyama H
  • Journal Title: Jpn J Clin Oncol Volume: 42(8) Issue: 8 Pages: 764-6
  • DOI: 10.1093/jjco/hys070
  • Peer Reviewed
• [Journal Article] Genome-wide identification of endothelial cell-enriched genes in the mouse embryo (2012)
  • Author(s): Takase H, Matsumoto H, Yamadera R, Kubota Y, Otsu A, Suzuki R, Ishitobi H, Mochizuki H, Kojima T, Takano S, Uchida K, Takahashi S, Ema M
  • Journal Title: Blood Volume: 120 Issue: 4 Pages: 914-923
  • DOI: 10.1182/blood-2011-12-398156
  • Peer Reviewed