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Epigenetics regulation of steroid syntesis related gene promoter regions in granulosa cells undergoing luteinization during ovulation in rats

Research Project

Project/Area Number 24791705
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Obstetrics and gynecology
Research InstitutionYamaguchi University

Principal Investigator

LEE Lifa  山口大学, 医学部附属病院, 助教 (90610668)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsエピジェネティクス / プロゲステロン合成 / 排卵 / 黄体 / 顆粒膜細胞
Research Abstract

The ovulatory LH surge rapidly alters the expression of steroidogenesis-related genes in granulosa cells. Steroidogenic acute regulatory protein (StAR) is rapidly induced and aromatase (Cyp19a1) is rapidly suppressed after the LH surge. The rapid shift of estrogen synthesis to progesterone synthesis after the LH surge is crucial for ovulation and the following luteinization. We revealed changes of histone modification status and chromatin structure in the StAR and Cyp19s1 promoter region in addition to DNA hypomethylation status of the promoter are closely associated with the rapid change of StAR and Cyp19a1 mRNA expression in granulosa cells undergoing luteinization during ovulation.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (16 results)

All 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (14 results) (of which Invited: 4 results)

  • [Journal Article] Change in histone modification and DNA methylation of the StAR and Cyp19a1 promoter region in granulosa cells undergoing kuteinization during ovulation in rats2013

    • Author(s)
      Lifa Lee, Hiromi asada, Fumie Kizuka, Isao Tamura, Ryo Maekawa, Toshiaki Taketani, Shun Sato, Yoshiaki Yamagata, Hiroshi Tamura, and Norihiro Sugino
    • Journal Title

      Endocrinology

      Volume: 154 Pages: 458-470

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Changes in Histone Modification and DNA Methylation of the StAR and Cyp19a1 Promoter Regions in Granulosa Cells Undergoing Luteinization during Ovulation In Rats2013

    • Author(s)
      Lee L
    • Journal Title

      Endocrinology

      Volume: 154 Issue: 1 Pages: 458-470

    • DOI

      10.1210/en.2012-1610

    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Changes in histone modification and DNA methylation of the Cyp11a1 promoter region and mRNA expression of histone modification enzymes in rat granulosa cells undergoing luteinization during ovulation2013

    • Author(s)
      Lifa Lee
    • Organizer
      SSR's 46th annual meeting
    • Place of Presentation
      Palais des congres de Montreal(Canada)
    • Related Report
      2013 Final Research Report
  • [Presentation] ラット顆粒膜細胞の黄体化に伴う P450scc(Cyp11a1) 遺伝子発現におけるepigenetics 制御機構の関与2013

    • Author(s)
      李 理華
    • Organizer
      第65回日本産科婦人科学会学術集会
    • Place of Presentation
      ロイトン札幌(北海道)
    • Related Report
      2013 Final Research Report
  • [Presentation] ラット顆粒膜細胞の黄体化に伴う Steroidogenic Acute Regulatory(StAR)遺伝子 Cyp19a1(aromatase) 遺伝子発現のepigenetics 制御の関与2013

    • Author(s)
      李 理華
    • Organizer
      第86回日本内分泌学会学術集会
    • Place of Presentation
      仙台国際センター(宮城県)
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] ラット顆粒膜細胞の黄体化に伴うSteroidogenic Acute Regulatory (StAR)遺伝子 Cyp19a1(aromatase)遺伝子発現のepigenetics制御の関与2013

    • Author(s)
      李 理華
    • Organizer
      第86回日本内分泌学会学術集会
    • Place of Presentation
      仙台国際センター(宮城県)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ラット顆粒膜細胞の黄体化に伴うP450scc(Cyp11a1)遺伝子発現 におけるepigenetics制御機構の関与2013

    • Author(s)
      李 理華
    • Organizer
      第65回日本産科婦人科学会学術集会
    • Place of Presentation
      ロイトン札幌(北海道)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Changes in histone modification and DNA methylation of the Cyp11a1 promoter region and mRNA expression of histone modification enzymes in rat granulosa cells undergoing luteinization during ovulation.2013

    • Author(s)
      Lifa Lee
    • Organizer
      SSR's 46th annual meeting
    • Place of Presentation
      Palais des congres de Montreal(Canada)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ラット顆粒膜細胞の黄体化に伴う Steroidogenic Acute Regulatory(StAR)遺伝子, Cyp19a1(aromatase) 遺伝子発現の epigenetics 制御の関与2012

    • Author(s)
      李 理華
    • Organizer
      第57回日本生殖医学会
    • Place of Presentation
      長崎ブリックホール(長崎県)
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] ラット顆粒膜細胞の黄体化に伴う Steroidogenic Acute Regulatory(StAR)遺伝子, Cyp19a1(aromatase) 遺伝子発現の epigenetics 制御の関与2012

    • Author(s)
      李 理華
    • Organizer
      第105回繁殖生物学会
    • Place of Presentation
      筑波大学大学会館(茨城県)
    • Related Report
      2013 Final Research Report
  • [Presentation] ラット顆粒膜細胞の黄体化に伴う Steroidogenic Acute Regulatory(StAR)遺伝子, Cyp19a1(aromatase) 遺伝子発現の epigenetics 制御の関与2012

    • Author(s)
      李 理華
    • Organizer
      第6回日本エピジェネティクス研究会
    • Place of Presentation
      学術総合センター(東京都)
    • Related Report
      2013 Final Research Report
  • [Presentation] ラット顆粒膜細胞の黄体化に伴う Steroidogenic Acute Regulatory(StAR)遺伝子, Cyp19a1(aromatase) 遺伝子発現の epigenetics 制御の関与2012

    • Author(s)
      李 理華
    • Organizer
      第85回日本内分泌学会
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Related Report
      2013 Final Research Report
  • [Presentation] ラット顆粒膜細胞の黄体化に伴うSteroidogenic Acute Regulatory (StAR)遺伝子、Cyp19a1(aromatase)遺伝子発現のepigenetics制御の関与2012

    • Author(s)
      李 理華
    • Organizer
      第6回日本エピジェネティクス研究会
    • Place of Presentation
      東京(学術総合センター)
    • Related Report
      2012 Research-status Report
  • [Presentation] ラット顆粒膜細胞の黄体化に伴うSteroidogenic Acute Regulatory (StAR)遺伝子、Cyp19a1(aromatase)遺伝子発現のepigenetics制御の関与2012

    • Author(s)
      李 理華
    • Organizer
      第85回日本内分泌学会
    • Place of Presentation
      名古屋(名古屋国際会議場)
    • Related Report
      2012 Research-status Report
  • [Presentation] ラット顆粒膜細胞の黄体化に伴うSteroidogenic Acute Regulatory (StAR)遺伝子、Cyp19a1(aromatase)遺伝子発現のepigenetics制御の関与2012

    • Author(s)
      李 理華
    • Organizer
      第105回 繁殖生物学会
    • Place of Presentation
      筑波(筑波大学大学会館)
    • Related Report
      2012 Research-status Report
  • [Presentation] ラット顆粒膜細胞の黄体化に伴うSteroidogenic Acute Regulatory (StAR)遺伝子、Cyp19a1(aromatase)遺伝子発現のepigenetics制御の関与2012

    • Author(s)
      李 理華
    • Organizer
      第57回日本生殖医学会
    • Place of Presentation
      長崎(長崎ブリックホール)
    • Related Report
      2012 Research-status Report
    • Invited

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Published: 2013-05-31   Modified: 2019-07-29  

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