Epigenetics regulation of steroid syntesis related gene promoter regions in granulosa cells undergoing luteinization during ovulation in rats

• Project/Area Number: 24791705
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Yamaguchi University
• Principal Investigator: LEE Lifa 山口大学, 医学部附属病院, 助教 (90610668)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: エピジェネティクス / プロゲステロン合成 / 排卵 / 黄体 / 顆粒膜細胞

Research Abstract

The ovulatory LH surge rapidly alters the expression of steroidogenesis-related genes in granulosa cells. Steroidogenic acute regulatory protein (StAR) is rapidly induced and aromatase (Cyp19a1) is rapidly suppressed after the LH surge. The rapid shift of estrogen synthesis to progesterone synthesis after the LH surge is crucial for ovulation and the following luteinization. We revealed changes of histone modification status and chromatin structure in the StAR and Cyp19s1 promoter region in addition to DNA hypomethylation status of the promoter are closely associated with the rapid change of StAR and Cyp19a1 mRNA expression in granulosa cells undergoing luteinization during ovulation.

Research Products

• [Journal Article] Change in histone modification and DNA methylation of the StAR and Cyp19a1 promoter region in granulosa cells undergoing kuteinization during ovulation in rats (2013)
  • Author(s): Lifa Lee, Hiromi asada, Fumie Kizuka, Isao Tamura, Ryo Maekawa, Toshiaki Taketani, Shun Sato, Yoshiaki Yamagata, Hiroshi Tamura, and Norihiro Sugino
  • Journal Title: Endocrinology Volume: 154 Pages: 458-470
  • Peer Reviewed
• [Journal Article] Changes in Histone Modification and DNA Methylation of the StAR and Cyp19a1 Promoter Regions in Granulosa Cells Undergoing Luteinization during Ovulation In Rats (2013)
  • Author(s): Lee L
  • Journal Title: Endocrinology Volume: 154 Issue: 1 Pages: 458-470
  • DOI: 10.1210/en.2012-1610
  • Peer Reviewed
• [Presentation] Changes in histone modification and DNA methylation of the Cyp11a1 promoter region and mRNA expression of histone modification enzymes in rat granulosa cells undergoing luteinization during ovulation (2013)
  • Author(s): Lifa Lee
  • Organizer: SSR's 46th annual meeting
  • Place of Presentation: Palais des congres de Montreal(Canada)
• [Presentation] ラット顆粒膜細胞の黄体化に伴う P450scc(Cyp11a1) 遺伝子発現におけるepigenetics 制御機構の関与 (2013)
  • Author(s): 李 理華
  • Organizer: 第65回日本産科婦人科学会学術集会
  • Place of Presentation: ロイトン札幌(北海道)
• [Presentation] ラット顆粒膜細胞の黄体化に伴う Steroidogenic Acute Regulatory(StAR)遺伝子 Cyp19a1(aromatase) 遺伝子発現のepigenetics 制御の関与 (2013)
  • Author(s): 李 理華
  • Organizer: 第86回日本内分泌学会学術集会
  • Place of Presentation: 仙台国際センター(宮城県)
  • Invited
• [Presentation] ラット顆粒膜細胞の黄体化に伴うSteroidogenic Acute Regulatory (StAR)遺伝子 Cyp19a1(aromatase)遺伝子発現のepigenetics制御の関与 (2013)
  • Author(s): 李 理華
  • Organizer: 第86回日本内分泌学会学術集会
  • Place of Presentation: 仙台国際センター(宮城県)
  • Invited
• [Presentation] ラット顆粒膜細胞の黄体化に伴うP450scc(Cyp11a1)遺伝子発現 におけるepigenetics制御機構の関与 (2013)
  • Author(s): 李 理華
  • Organizer: 第65回日本産科婦人科学会学術集会
  • Place of Presentation: ロイトン札幌(北海道)
• [Presentation] Changes in histone modification and DNA methylation of the Cyp11a1 promoter region and mRNA expression of histone modification enzymes in rat granulosa cells undergoing luteinization during ovulation. (2013)
  • Author(s): Lifa Lee
  • Organizer: SSR's 46th annual meeting
  • Place of Presentation: Palais des congres de Montreal(Canada)
• [Presentation] ラット顆粒膜細胞の黄体化に伴う Steroidogenic Acute Regulatory(StAR)遺伝子, Cyp19a1(aromatase) 遺伝子発現の epigenetics 制御の関与 (2012)
  • Author(s): 李 理華
  • Organizer: 第57回日本生殖医学会
  • Place of Presentation: 長崎ブリックホール(長崎県)
  • Invited
• [Presentation] ラット顆粒膜細胞の黄体化に伴う Steroidogenic Acute Regulatory(StAR)遺伝子, Cyp19a1(aromatase) 遺伝子発現の epigenetics 制御の関与 (2012)
  • Author(s): 李 理華
  • Organizer: 第105回繁殖生物学会
  • Place of Presentation: 筑波大学大学会館(茨城県)
• [Presentation] ラット顆粒膜細胞の黄体化に伴う Steroidogenic Acute Regulatory(StAR)遺伝子, Cyp19a1(aromatase) 遺伝子発現の epigenetics 制御の関与 (2012)
  • Author(s): 李 理華
  • Organizer: 第6回日本エピジェネティクス研究会
  • Place of Presentation: 学術総合センター(東京都)
• [Presentation] ラット顆粒膜細胞の黄体化に伴う Steroidogenic Acute Regulatory(StAR)遺伝子, Cyp19a1(aromatase) 遺伝子発現の epigenetics 制御の関与 (2012)
  • Author(s): 李 理華
  • Organizer: 第85回日本内分泌学会
  • Place of Presentation: 名古屋国際会議場(愛知県)
• [Presentation] ラット顆粒膜細胞の黄体化に伴うSteroidogenic Acute Regulatory (StAR)遺伝子、Cyp19a1(aromatase)遺伝子発現のepigenetics制御の関与 (2012)
  • Author(s): 李 理華
  • Organizer: 第6回日本エピジェネティクス研究会
  • Place of Presentation: 東京(学術総合センター)
• [Presentation] ラット顆粒膜細胞の黄体化に伴うSteroidogenic Acute Regulatory (StAR)遺伝子、Cyp19a1(aromatase)遺伝子発現のepigenetics制御の関与 (2012)
  • Author(s): 李 理華
  • Organizer: 第85回日本内分泌学会
  • Place of Presentation: 名古屋(名古屋国際会議場)
• [Presentation] ラット顆粒膜細胞の黄体化に伴うSteroidogenic Acute Regulatory (StAR)遺伝子、Cyp19a1(aromatase)遺伝子発現のepigenetics制御の関与 (2012)
  • Author(s): 李 理華
  • Organizer: 第105回 繁殖生物学会
  • Place of Presentation: 筑波(筑波大学大学会館)
• [Presentation] ラット顆粒膜細胞の黄体化に伴うSteroidogenic Acute Regulatory (StAR)遺伝子、Cyp19a1(aromatase)遺伝子発現のepigenetics制御の関与 (2012)
  • Author(s): 李 理華
  • Organizer: 第57回日本生殖医学会
  • Place of Presentation: 長崎(長崎ブリックホール)
  • Invited