Comprehensive analysis of mitochondria-related genes during gametogenesis and it's application to reproductive medicine
| Project/Area Number |
24791720
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Keio University |
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Principal Investigator |
SATO Suguru 慶應義塾大学, 医学部, 助教 (40383898)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | ミトコンドリア / 加齢 / 減数分裂 / 卵子 / 着床前遺伝子診断 / ミトコンドリア病 |
|---|
| Research Abstract |
We have obtained the following findings during the research period: (1)For detection of heteroplasmy for mtDNA mutations associated with the diseases, quick, simple and cheap diagnostic system using whole genome amplification (WGA)-PCR-RFLP-Image J simple was established. (2)As a result of chromosome aneuploidy analysis on single cell, 63% of the embryo which developed in the case of preimplantation diagnosis were revealed aneuploid. (3)As a effect of examination of the amplification efficiency of WGA, multi-locus simoultananeous analysis of targeted gene and STR markers closely linked to the gene enabled preimplantation genetic haplotyping.
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Report
(3 results)
Research Products
(23 results)
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[Presentation] 全ゲノム増幅法を用いた遺伝子ハプロタイピングを基盤とする新たな着床前診断の開発研究2013
Author(s)
佐藤卓, 末岡浩, 佐藤健二, 中林章, 水口雄貴, 高橋香織, 磯部まり子, 泉 陽子, 大澤淑子, 青木大輔, 吉村泰典
Organizer
第65回日本産科婦人科学会学術講演会
Place of Presentation
ロイトン札幌, ホテルさっぽろ芸文館(旧北海道厚生年金会館), 札幌プリンスホテル (札幌市)
Year and Date
2013-05-10
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[Presentation] 全ゲノム増幅法を用いた遺伝子ハプロタイピングを基盤とする新たな着床前診断の開発研究2013
Author(s)
佐藤 卓, 末岡 浩, 中林 章, 泉 陽子, 水口雄貴, 高橋香織, 櫻井友義, 佐藤健二, 大澤淑子, 青木大輔, 吉村泰典
Organizer
第65回日本産科婦人科学会学術講演会
Place of Presentation
札幌・ロイトン札幌
Related Report
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