| Project/Area Number |
24791800
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
TAKEI Shin 和歌山県立医科大学, 医学部, 博士研究員 (40347589)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | バイオフィルム / 抗菌薬 / 抗菌薬感受性 / インフルエンザ菌 |
|---|
| Outline of Final Research Achievements |
Nontypeable Haemophilus influenzae (NTHi) makes the clinical course of acute otitis media (AOM) intractable by forming biofilm that may hamper the clearance of the bacteria from middle ear cavity. In this study, we evaluated the minimum biofilm eradication concentration (MBEC) of antimicrobial agents against biofilm-forming NTHi strains.
Twelve NTHi strains isolated from middle ear fluids (MEFs) of Japanese children with intractable AOM prior to antimicrobial treatments were evaluated for MBEC of fluoroquinolones in comparison with those of ß-lactams and macrolides. AMPC and CDTR required much higher concentration, i.e., high MBECs, to suppress the biofilm formation of NTHi. In contrast, fluoroquinolones followed by macrolides showed lower MBECs. The MBEC would be the good parameter to infer the efficacies of antimicrobials against NTHi in biofilm.
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