| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Outline of Final Research Achievements |
Light microscopy revealed the hexagonal shape of RPE cells with an overlying fibrous membrane, suggesting upside-down polarity of RPE and the sheet was easily detached from the bottom of the plate by the gentle pipetting technique. The RPE sheet expressed occludin and ZO-1 as tight junction markers as well as actin, collagen type IV and elastin as a Bruch’s membrane component in Western blotting. In SEM images, the upper side showed microvilli and lamellipodia of RPE cells and, thereafter, Bruch’s membrane-like structures involving elastic fibers and collagen. TEM disclosed the tight junctions of the RPE cells. Confocal immunofluorescence microscopy showed the expression of ZO-1 and occludin among RPE cells. The RPE sheet manufactured by our original method was well-structured as an implantable sheet with Bruch’s membrane and tight junction. This RPE sheet may have a potential as a better option for RPE transplantation.
|
