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Analysis of miRNA signatures in eyes with EAU in rat

Research Project

Project/Area Number 24791873
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Ophthalmology
Research InstitutionKyorin University

Principal Investigator

WATANABE Takayo  杏林大学, 医学部, 助教 (90458901)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsぶどう膜炎 / microRNA
Research Abstract

Micro-RNAs (miRNAs) are non-coding, 21-24 nucleotide long RNAs that have recently emerged as important posttranscriptional regulators of gene expression. In the present study, we investigated the changes of miRNA expression of retina during the development of EAU in rats. Inflammation in anterior segment by biomicroscopic evaluation peaked on day 14. The expression of IL-1beta and MCP-1 in aqueous humor from eyes with EAU peaked on day 14. Microarray analysis demonstrated that the expression of mir-223, 142, 21, and 146 in retina was elevated on day 14 than on day 0 (baseline), and the expression of these miRNAs declined on day 21, whereas the expression of mir-181, 183, 124, and 331 was reduced on day 14. These results were confirmed by qRT-PCR method. The changes of miRNA expression paralleled clinical course of EAU in rat. These findings suggest that these up-regulated or down-regulated miRNAs may contribute to the development and regulation of EAU in rat.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (19 results)

All 2014 2013 2012

All Journal Article (4 results) (of which Peer Reviewed: 3 results) Presentation (11 results) Book (4 results)

  • [Journal Article] 先天白内障眼の角膜乱視2014

    • Author(s)
      渡辺交世, 松木奈央子, 柳沼重晴, 永本敏之
    • Journal Title

      日本眼科学会雑誌

      Volume: 108 Pages: 98-103

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Journal Article] Enhanced depth imaging optical coherence tomography of the choroid in new-onset acute posterior scleritis2013

    • Author(s)
      Hirukawa K, Keino H, Watanabe T, Okada AA
    • Journal Title

      Graefes Arch Clin Exp Ophthalmol

      Volume: 251 Issue: 9 Pages: 2273-2275

    • DOI

      10.1007/s00417-013-2265-z

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced depth imaging optical coherence tomography of the choroid in recurrent unilateral posterior scleritis2013

    • Author(s)
      Taki W, Keino H, Watanabe T, Okada AA
    • Journal Title

      Graefes Arch Clin Exp Ophthalmol

      Volume: 251 Issue: 3 Pages: 1003-1004

    • DOI

      10.1007/s00417-012-1972-1

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Enhanced depth imaging optical coherence tomography of the choroid in Vogt-Koyanagi-Harada disease2012

    • Author(s)
      Nakayama M, Keino H, Okada AA, Watanabe T, Taki W, Inoue M, Hirakata A.
    • Journal Title

      Retina

      Volume: 32 Issue: 10 Pages: 2061-2069

    • DOI

      10.1097/iae.0b013e318256205a

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Clinical characteristics of patients diagnosed with presumed diabetic iritis2014

    • Author(s)
      Watanabe T, Hirukawa K, Keino H, Taki W, Echizen N, Okada AA
    • Organizer
      International ocular inflammation society meeting
    • Place of Presentation
      Valencia, Spain
    • Related Report
      2013 Annual Research Report
  • [Presentation] 杏林アイセンターにおける小児および若年者のぶどう膜炎の統計2013

    • Author(s)
      慶野博, 中島史絵, 渡辺交世, 瀧和歌子、岡田アナベルあやめ
    • Organizer
      第67回日本臨床眼科学会
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 網膜中心静脈閉塞症から抗リン脂質抗体症候群の診断に至った一例2013

    • Author(s)
      渡邊交世, 慶野博, 瀧 和歌子, 越前成旭, 肥留川京子,岡田アナベルあやめ
    • Organizer
      第47回日本眼炎症学会
    • Place of Presentation
      大阪
    • Related Report
      2013 Final Research Report
  • [Presentation] 実験的自己免疫性ぶどう膜網膜炎 (EAU) における眼局所の microRNAの発現2013

    • Author(s)
      慶野博, 渡辺交世, 宮東昭彦, 川上速人,岡田アナベルあやめ
    • Organizer
      第47回日本眼炎症学会
    • Place of Presentation
      大阪
    • Related Report
      2013 Final Research Report
  • [Presentation] Clinical characteris tics of patients diagnosed with presumed2013

    • Author(s)
      Takayo Watanabe, Kyoko Hirukawa, Hiros hi Keino, Wakako Taki, Nariaki Echizen, Annabelle A. Okada
    • Organizer
      diabetic iritis IOIS meeting
    • Place of Presentation
      Valencia Spain
    • Related Report
      2013 Final Research Report
  • [Presentation] 網膜中心静脈閉塞症から抗リン脂質抗体症候群の診断に至った一例2013

    • Author(s)
      渡邊交世、慶野博、瀧 和歌子、越前成旭、肥留川京子、岡田アナベルあやめ
    • Organizer
      第47回 日本眼炎症学会
    • Place of Presentation
      大阪
    • Related Report
      2013 Annual Research Report
  • [Presentation] EDI-OCT を用いた交感性眼炎回復期の脈絡膜厚の評価2012

    • Author(s)
      慶野博, 渡辺交世, 瀧 和歌子,岡田アナベルあやめ
    • Organizer
      第66回日本臨床眼科学会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] 網膜動静脈閉塞症に対してステロイドパルス療法が奏功した SLE 網膜症の 1例2012

    • Author(s)
      肥留川京子, 慶野博, 渡辺交世, 瀧和歌子, 平形明人,岡田アナベルあやめ
    • Organizer
      第46回日本眼炎症学会
    • Place of Presentation
      横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] インフリキシマブ長期投与ベーチェット病患者の蛍光眼底造影像の推移2012

    • Author(s)
      慶野博, 渡辺交世, 瀧和歌子,岡田アナベルあやめ
    • Organizer
      第46回日本眼炎症学会
    • Place of Presentation
      横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] インフリキシマブ治療を導入した若年性ベーチェット病ぶどう膜網膜炎の2症例2012

    • Author(s)
      渡辺交世, 慶野博, 瀧和歌子, 越前成旭,岡田アナベルあやめ
    • Organizer
      第116回日本眼科学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 網膜動静脈閉塞症に対してステロイドパルス療法が奏功したSLE網膜症の1例2012

    • Author(s)
      肥留川京子、慶野博、渡辺交世、瀧和歌子、平形明人、岡田アナベルあやめ
    • Organizer
      第46回日本眼炎症学会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Book] 眼科疾患最新の治療2013-2015梅毒, 結核2013

    • Author(s)
      渡邊交世
    • Publisher
      南江堂
    • Related Report
      2013 Final Research Report
  • [Book] 眼科疾患ビジュアルブックChapter 9 水晶体疾患2013

    • Author(s)
      渡邊交世
    • Publisher
      学研メディカル秀潤社
    • Related Report
      2013 Final Research Report
  • [Book] 眼科疾患ビジュアルブック2013

    • Author(s)
      渡邊交世
    • Total Pages
      8
    • Publisher
      学研メディカル秀潤社
    • Related Report
      2013 Annual Research Report
  • [Book] 眼科疾患最新の治療2013-20152013

    • Author(s)
      渡邊交世
    • Total Pages
      1
    • Publisher
      南江堂
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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