| Project/Area Number |
24791949
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Emergency medicine
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
MAEDA Sachiko 京都府立医科大学, 医学部附属病院, 病院助教 (90529512)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 遺伝子治療 |
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| Research Abstract |
Thrombotic disorders by platelet dysfunction are frequently observed after cardiopulmonary bypass. We tested the hypothesis that differences in intraplatelet microRNA expression are related with intraplatelet Bax and platelet surface glycoprotein Ib expression, which subsequently affect perioperative blood loss.
In in vitro studies, we found that increase in intraplatelet miR-886-5p in thrombin-stimulated platelets has a strong relationship with preservation of platelet surface glycoprotein Ib expression and inhibition of intraplatelet Bax expression. In in vivo studies, we found a relationship between intraplatelet miR-886-5p expression, and platelet surface glycoprotein Ib and intraplatelet Bax expression in patients undergoing cardiac surgery after cardiopulmonary bypass. Patients showing increase in intraplatelet miR-886-5p expression had a tendency of decreased postoperative blood loss. Further studies are required to determine the complete clinical relevance of this relationship.
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