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The elucidation of functional regulation of osteoclasts and improvements of bone metabolism by estrogen and estrogen responsive genes

Research Project

Project/Area Number 24791963
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Morphological basic dentistry
Research InstitutionHiroshima University

Principal Investigator

HIYAMA Shinji  広島大学, 医歯薬保健学研究院(歯), 助教 (60314754)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords口腔解剖学 / 骨代謝 / エストロゲン / 破骨細胞 / エストロゲン応答遺伝子
Research Abstract

Estrogen inhibited bone resorption, but did not induce apoptosis of osteoclasts that derived from bone marrow cells of estrogen-treated male quails. Further, the morphology of osteoclasts and the expression of ATPase in these cells were affected by estrogen administration. As a result of DNA microarray, a lot of genes that were significantly up- or down-regulated by estrogen administration were identified in these osteoclasts. Osteoclastogenesis from bone marrow cells were suppressed by knock-down of some of genes with/without estrogen. Taken together with these results, estrogen has no effects on the apoptosis of medullary bone osteoclasts, but rather suppress bone resorption. Additionally, it is suggested that many genes that indicated significant regulation of expression are related to the inhibition of apoptosis as estrogen responsive genes.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (13 results)

All 2013 2012

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (7 results) (of which Invited: 1 results)

  • [Journal Article] Gene Expression Profiling of Avian Osteoclasts Treated with Estrogen for Exploring Unidentified Action(s) of Estrogen in Bone2013

    • Author(s)
      Shinji Hiyama, Yumiko Kadoyama, Mineo Watanabe, Takashi Uchida
    • Journal Title

      Journal of Bone and Mineral Reseach

      Volume: 28

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Estrogen is involved in osteoclast activity but neither in osteoclastogenesis nor in osteoclast apoptosis in avian medullary bone2013

    • Author(s)
      Shinji Hiyama, Yumiko Kadoyama, Takashi Uchida
    • Journal Title

      IBMS (International Bone and Mineral Society)

      Volume: 10

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 1. Gene Expression Profiling of Avian Osteoclasts Treated with Estrogen for Exploring Unidentified Action(s) of Estrogen in Bone.2013

    • Author(s)
      Hiyama S, Kadoyama Y, Watanabe M, Uchida T.
    • Journal Title

      J Bone Miner Res.

      Volume: 28 Issue: S1 Pages: S1-S1

    • DOI

      10.1002/jbmr.2201

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 2. Osteoclasts of avian medullary bone do not occur apoptosis and affect osteoclastogenesis, but are inhibited of function by estrogen.2013

    • Author(s)
      Hiyama S, Kadoyama Y, Uchida T.
    • Journal Title

      IBMS (International Bone and Mineral Society) BoneKEy.

      Volume: 10 Pages: S42-S117

    • DOI

      10.1038/bonekey.2013.69

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sequential Expression of Osteoblast Phenotypic Genes During Medullary Bone Formation and Resorption in Estrogen- Treated Male Japanese Quails2012

    • Author(s)
      SHINJI HIYAMA, TOSHIE SUGIYAMA, SEIJI KUSUHARA, TAKASHI UCHIDA
    • Journal Title

      Journal of Experimental Zoology (Molecular and Developmental Evolution)

      Volume: 318B Issue: 5 Pages: 344-352

    • DOI

      10.1002/jez.b.22451

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Evidence for estrogen receptor expression during medullary bone formation and resorption in estrogentreated male Japanese quails (Coturnix coturnix japonica)2012

    • Author(s)
      SHINJI HIYAMA, TOSHIE SUGIYAMA, SEIJI KUSUHARA, TAKASHI UCHIDA
    • Journal Title

      Journal of Veterinary Science

      Volume: 13(3) Issue: 3 Pages: 223-227

    • DOI

      10.4142/jvs.2012.13.3.223

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] The regulation of osteoclastogenesis by the alteration of bone marrow cells in avian medullary bone2013

    • Author(s)
      Shinji Hiyama, Yumiko Kadoyama, Takashi Uchida
    • Organizer
      Comparative Endocrinology of Calcium Regulation Workshop
    • Place of Presentation
      Kobe, Japan
    • Year and Date
      2013-05-28
    • Related Report
      2013 Final Research Report
  • [Presentation] Gene Expression Profiling of Avian Osteoclasts Treated with Estrogen for Exploring Unidentified Action(s) of Estrogen in Bone2013

    • Author(s)
      Shinji Hiyama, Yumiko Kadoyama, Mineo Watanabe, Takashi Uchida
    • Organizer
      American Society for Bone and Mineral Research Annual Meeting 2013
    • Place of Presentation
      Baltimore, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Estrogen is involved in osteoclast activity but neither in osteoclastogenesis nor in osteoclast apoptosis in avian medullary bone2013

    • Author(s)
      Shinji Hiyama, Yumiko Kadoyama, Takashi Uchida
    • Organizer
      IBMS (International Bone and Mineral Society)
    • Place of Presentation
      Kobe, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] 1. Gene Expression Profiling of Avian Osteoclasts Treated with Estrogen for Exploring Unidentified Action(s) of Estrogen in Bone.2013

    • Author(s)
      Hiyama S, Kadoyama Y, Watanabe M, Uchida T.
    • Organizer
      American Society for Bone and Mineral Research Annual Meeting 2013
    • Place of Presentation
      Baltimore, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] 2. Osteoclasts of avian medullary bone do not occur apoptosis and affect osteoclastogenesis, but are inhibited of function by estrogen.2013

    • Author(s)
      Hiyama S, Kadoyama Y, Uchida T.
    • Organizer
      IBMS (International Bone and Mineral Society)
    • Place of Presentation
      Kobe, Japan
    • Related Report
      2013 Annual Research Report
  • [Presentation] 3. The regulation of osteoclastogenesis by the alteration of bone marrow cells in avian medullary bone.2013

    • Author(s)
      Hiyama S, Kadoyama Y, Uchida T.
    • Organizer
      Invited speaker. Comparative Endocrinology of Calcium Regulation Workshop.
    • Place of Presentation
      Kobe, Japan
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 鳥類骨髄骨の破骨細胞および前駆細胞はエストロゲンでアポトーシスが誘導されない2013

    • Author(s)
      樋山伸二・門山裕美子・渡邉峰朗・内田隆
    • Organizer
      第118回解剖学会総会・全国学術集会
    • Place of Presentation
      サンポートホール高松・かがわ国際会議場
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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