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Transposon mutagenesis of biofilm-forming Rothia mucilaginosa

Research Project

Project/Area Number 24791975
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Morphological basic dentistry
Research InstitutionOsaka Dental University

Principal Investigator

YAMANE Kazuyoshi  大阪歯科大学, 歯学部, 講師 (40388369)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsバイオフィルム / Rothia mucilaginosa / 遺伝子
Research Abstract

Clinical isolate (strain DY-18) of Rothia mucilaginosa from persistent apical periodontitis produce a large amount of exopolysaccharide (EPS). In this study, to identify the genes involved in biofilm formation in R. mucilaginosa, we established EPS non-producing mutants from strain DY-18 by random mutagenesis. The viscosity of culture medium in these mutants were lower than the parent strain culture. Scanning electron microscopic observations on cell surface revealed that these mutans lacked or had significantly decreased meshwork-like structures, which is typical for biofilm-forming bacteria. We compared genes expression profiles of strain DY-18 between in static culture producing large amount of EPS, and shake culture, EPS non-producing condition, by microarray. The results in this study indicate that expression of stress response genes in the static culture is higher than that in the shaking growth condition and suggest that these genes might involve in biofilm formation.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (37 results)

All 2013 2012 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (25 results)

  • [Journal Article] 口腔感染症の病院論が変わった2013

    • Author(s)
      山根一芳, 円山由郷, 南部隆之, 真下千穂, 山中武志, 福島久典
    • Journal Title

      歯界展望特別号

      Volume: 189

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 口腔アーキアによって疾患は起こるか?2013

    • Author(s)
      円山由郷, 山根一芳, 南部隆之, 真下千穂, 山中武志, 福島久典
    • Journal Title

      歯界展望特別号

      Volume: 189

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 広宿主域接合プラスミドpJRD215 を基盤にした新規プラスミドの作製と口腔Actinomyces 属細菌への応用2013

    • Author(s)
      真下千穂, 南部隆之, 円山由郷, 山根一芳, 山中武志, 福島久典
    • Journal Title

      Bacterial adherence & biofilm

      Volume: 26 Pages: 61-64

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] バイオフィルム形成Streptococcus constellatus のゲノム解析2013

    • Author(s)
      安岡大志, 山根一芳, 福島久典
    • Journal Title

      歯科医学

      Volume: 76(2) Pages: 1-10

    • NAID

      10031138687

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biofilm-forming capacity on clinically isolated Streptococcus constellatus from an odontogenic subperiosteal abscess lesion2013

    • Author(s)
      Yamanaka T, Furukawa T, Yamane K, Nambu T, Mashimo C, Maruyama H, Inoue J, Kamei M, Yasuoka H, Horiike S, Leung KP, Fukushima H
    • Journal Title

      Journal of Bacteriology & Parasitology

      Volume: 4 Issue: 01 Pages: 1000160-1000160

    • DOI

      10.4172/2155-9597.1000160

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Biofilm formation of Actinomyces oris strain MG-1 on an orthodontic wire2013

    • Author(s)
      Omae A, Yamane K, Matsumoto N
    • Journal Title

      Journal of Osaka Dental University

      Volume: 47(1) Pages: 75-82

    • NAID

      110009603281

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the genes involved in the biofilm-like structures on Actinomyces oris K20, a clinical isolate from an apical lesion2013

    • Author(s)
      Mashimo C, Kamitani M, Nambu T, Yamane K, Yamanaka T, Tatami T, Inoue J, Kamei M, Morita S, Fukushima H
    • Journal Title

      Jounal of Endodontics

      Volume: 39(1) Issue: 1 Pages: 44-48

    • DOI

      10.1016/j.joen.2012.08.009

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Pathogenicity of exopolysaccharide-producing Actinomyces oris isolated from an apical abscess lesion2013

    • Author(s)
      Yamane K, Nambu T, Yamanaka T, Ishihara K, Tatami T, Mashimo C, Walker CB, Leung K.-P., Fukushima H
    • Journal Title

      International Endodontic Journal

      Volume: 46(2) Issue: 2 Pages: 145-154

    • DOI

      10.1111/j.1365-2591.2012.02099.x

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of disulphide stress-responsive extracytoplasmic function sigma factors in Rothia mucilaginosa2013

    • Author(s)
      Nambu T, Yamane K, Yamanaka T, Mashimo C, Maruyama H, Yoshida M, Hayashi H, Fukushima H
    • Journal Title

      Archives of Oral Biology

      Volume: 58(6) Issue: 6 Pages: 681-689

    • DOI

      10.1016/j.archoralbio.2012.10.017

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Pathogenicity of exopolysaccharide-producing Actinomyces oris isolated from an apical abscess lesion2013

    • Author(s)
      Yamane K, Nambu T, Yamanaka T, Ishihara K, Tatami T, Mashimo C, Walker CB, Leung K.-P., Fukushima H
    • Journal Title

      International Endodontic Journal

      Volume: 46(2) Pages: 145-154

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] バイオフィルム形成Streptococcus constellatusのゲノム解析2013

    • Author(s)
      安岡大志,山根一芳,福島久典
    • Journal Title

      歯科医学

      Volume: 76(2)

    • NAID

      10031138687

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Analysis of genes involved in exopolysaccharide production of Rothia mucilaginosa clinically isolated from a persistent apical periodontitis lesion2012

    • Author(s)
      Fujihira T, Yamane K, Yoshida M, Nambu T, Hayashi H
    • Journal Title

      Journal of Osaka Dental University

      Volume: 46(1) Pages: 157-164

    • NAID

      110009436547

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Prevotella intermedia の熱ショックタンパクとバイオフィルム形成2013

    • Author(s)
      山中武志, 山根一芳, 南部隆之, 真下千穂, 福島久典
    • Organizer
      第55回歯科基礎医学会学術大会
    • Place of Presentation
      岡山市
    • Year and Date
      2013-09-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 口腔アクチノバクテリアは硝酸依存的にP. gingivalis を殺す2013

    • Author(s)
      南部隆之, 真下千穂, 山根一芳, 山中武志, 福島久典
    • Organizer
      第55回歯科基礎医学会学術大会
    • Place of Presentation
      岡山市
    • Year and Date
      2013-09-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Prevotella intermedia のチタンプレート上でのバイオフィルム形成2013

    • Author(s)
      山中武志, 小幡登, 円山由郷, 南部隆之, 真下千穂, 山根一芳, 福島久典
    • Organizer
      第539回大阪歯科学会例会
    • Place of Presentation
      枚方市
    • Year and Date
      2013-06-08
    • Related Report
      2013 Final Research Report
  • [Presentation] Genetic analysis of biofilm-forming Streptococcus constellatus2013

    • Author(s)
      Yamane K, Yamanaka T, Mashimo C, Nambu T, Maruyama H, Walker CB, Leung K-P, Fukushima H
    • Organizer
      IADR/AADR/CADR General Session & Exhibition
    • Place of Presentation
      Seattle, Washington, USA
    • Year and Date
      2013-03-21
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 口腔アクチノマイセスにおけるバイオフィルム欠損株の同定と特徴づけ2013

    • Author(s)
      南部隆之, 真下千穂, 山根一芳, 山中武志, 福島久典
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      千葉市
    • Year and Date
      2013-03-18
    • Related Report
      2013 Final Research Report
  • [Presentation] pJRD215 を基盤にしたクローニング・発現用プラスミドの作製とActinomyces 属細菌への応用2013

    • Author(s)
      真下千穂, 南部隆之, 山根一芳, 山中武志, 福島久典
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      千葉市
    • Year and Date
      2013-03-18
    • Related Report
      2013 Final Research Report
  • [Presentation] Characterization of Tn5 insertion mutants of oral Actinomyces affected in biofilm formation2012

    • Author(s)
      Nambu T, Mashimo C, Yamane K, Yamanaka T, Maruyama H, Fukushima H
    • Organizer
      Biofilms 5
    • Place of Presentation
      Paris, France
    • Year and Date
      2012-12-12
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] バイオフィルム形成Streptococcus constellatus のゲノム解析2012

    • Author(s)
      安岡大志, 山根一芳, 福島久典
    • Organizer
      大阪歯科学会例会
    • Place of Presentation
      枚方市
    • Year and Date
      2012-12-08
    • Related Report
      2013 Final Research Report
  • [Presentation] Sucrose independent exopolysaccharide productivity and biofilm formation of oral commensal bacteria as pathogenesis of chronic inflammation in the oral cavity2012

    • Author(s)
      Yamanaka T, Yamane K, Mashimo C, Nambu T, Maruyama H, Leung KP, Fukushima H
    • Organizer
      Clinical Microbiology and Microbial Genomics
    • Place of Presentation
      San Antonio, USA
    • Year and Date
      2012-11-12
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 口腔アーキアによって疾患は起こるか?2012

    • Author(s)
      円山由郷, 山根一芳, 南部隆之, 真下千穂, 山中武志, 福島久典
    • Organizer
      日本歯科医学会総会
    • Place of Presentation
      大阪市
    • Year and Date
      2012-11-10
    • Related Report
      2013 Final Research Report
  • [Presentation] 口腔感染症の病因論が変わった2012

    • Author(s)
      山根一芳, 円山由郷, 南部隆之, 真下千穂, 山中武志, 福島久典
    • Organizer
      日本歯科医学会総会
    • Place of Presentation
      大阪市
    • Year and Date
      2012-11-10
    • Related Report
      2013 Final Research Report
  • [Presentation] 矯正線表面へのActinomyces oris MG‐1 株の付着性2012

    • Author(s)
      大前有紀, 山根一芳, 南部隆之, 松本尚之
    • Organizer
      日本矯正歯科学会大会
    • Place of Presentation
      盛岡市
    • Year and Date
      2012-09-28
    • Related Report
      2013 Final Research Report
  • [Presentation] Development of new plasmids for cloning and expression in oral Actinomyces spp2012

    • Author(s)
      Mashimo C, Nambu T, Maruyama H, Yamane K, Yamanaka T, Fukushima H
    • Organizer
      The 4th EMBO Meeting 2012
    • Place of Presentation
      Nice, France
    • Year and Date
      2012-09-24
    • Related Report
      2013 Final Research Report
  • [Presentation] 口腔膿瘍から分離したStreptococcus intermedius のゲノム解析2012

    • Author(s)
      山根一芳, 南部隆之, 真下千穂, 山中武志, 福島久典
    • Organizer
      歯科基礎医学会学術大会・総会
    • Place of Presentation
      郡山市
    • Year and Date
      2012-09-16
    • Related Report
      2013 Final Research Report
  • [Presentation] バイオフィルム形成Streptococcus intermedius のゲノム解析2012

    • Author(s)
      山根一芳, 山中武志, 真下千穂, 吉田 匡宏, 林宏行, 南部隆之, 円山由郷, 福島久典
    • Organizer
      日本歯科保存学会
    • Place of Presentation
      宜野湾市
    • Year and Date
      2012-06-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Prevotella intermediaのチタンプレート上でのバイオフィルム形成

    • Author(s)
      山中 武志, 小幡 登, 円山 由郷, 南部 隆之, 真下 千穂, 山根 一芳, 福島 久典
    • Organizer
      第539回大阪歯科学会例会
    • Place of Presentation
      枚方市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development of new plasmids for cloning and expression in oral Actinomyces spp.

    • Author(s)
      Mashimo C, Nambu T, Maruyama H, Yamane K, Yamanaka T, Fukushima H
    • Organizer
      The 4th EMBO Meeting 2012
    • Place of Presentation
      Nice, France.
    • Related Report
      2012 Research-status Report
  • [Presentation] 口腔アクチノマイセスにおけるバイオフィルム欠損株の同定と特徴づけ

    • Author(s)
      南部 隆之, 真下 千穂, 山根 一芳, 山中 武志, 福島 久典
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      千葉市
    • Related Report
      2012 Research-status Report
  • [Presentation] pJRD215を基盤にしたクローニング・発現用プラスミドの作製とActinomyces属細菌への応用.

    • Author(s)
      真下 千穂, 南部 隆之, 山根 一芳, 山中 武志, 福島 久典
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      千葉市
    • Related Report
      2012 Research-status Report
  • [Presentation] バイオフィルム形成Streptococcus constellatusのゲノム解析

    • Author(s)
      安岡 大志, 山根 一芳, 福島 久典
    • Organizer
      大阪歯科学会例会
    • Place of Presentation
      枚方市
    • Related Report
      2012 Research-status Report
  • [Presentation] 口腔アーキアによって疾患は起こるか?

    • Author(s)
      円山 由郷, 山根 一芳, 南部 隆之, 真下 千穂, 山中 武志, 福島 久典
    • Organizer
      日本歯科医学会総会
    • Place of Presentation
      大阪市
    • Related Report
      2012 Research-status Report
  • [Presentation] 口腔感染症の病因論が変わった

    • Author(s)
      山根 一芳, 円山 由郷, 南部 隆之, 真下 千穂, 山中 武志, 福島 久典
    • Organizer
      日本歯科医学会総会
    • Place of Presentation
      大阪市
    • Related Report
      2012 Research-status Report
  • [Presentation] 矯正線表面へのActinomyces oris MG‐1株の付着性

    • Author(s)
      大前 有紀, 山根 一芳, 南部 隆之, 松本 尚之
    • Organizer
      日本矯正歯科学会大会
    • Place of Presentation
      盛岡市
    • Related Report
      2012 Research-status Report
  • [Presentation] 口腔膿瘍から分離したStreptococcus intermediusのゲノム解析

    • Author(s)
      山根 一芳, 南部 隆之, 真下 千穂, 山中 武志, 福島 久典
    • Organizer
      歯科基礎医学会学術大会・総会
    • Place of Presentation
      郡山市
    • Related Report
      2012 Research-status Report
  • [Presentation] バイオフィルム形成Streptococcus intermediusのゲノム解析

    • Author(s)
      山根 一芳, 山中 武志, 真下 千穂, 吉田 匡宏, 林 宏行, 南部 隆之, 円山 由郷, 福島 久典
    • Organizer
      日本歯科保存学会
    • Place of Presentation
      宜野湾市
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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