A novel preventive technique of dental diseases based on photo-dynamic anti-microbial therapy utilizing plaque discolinsg liquid

• Project/Area Number: 24791977
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional basic dentistry
• Research Institution: Tohoku University
• Principal Investigator: NAKAMURA Keisuke 東北大学, 歯学研究科(研究院), 助教 (30431589)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 光線力学療法 / 殺菌 / 一重項酸素 / 水酸化ラジカル / プラーク染色剤 / ポリフェノール / レーザー

Outline of Final Research Achievements

Dyes contained in plaque disclosing liquid can be activated by photo-irradiation with a specific wavelength. The activated dyes generate singlet oxygen, a type of reactive oxygen species, when they return to the ground state. Since singlet oxygen has strong oxidative effect, it exerts bactericidal activity when reacts with bacteria. In the present study, it was demonstrated that the photodynamic antimicrobial therapy exerted strong bactericidal action on cariogenic bacteria (Streptococcus mutans). In addition, it was found that photo-irradiation of polyphenol aqueous solution also exerted bactericidal effect. Thus, it is suggested that this technique can be used a novel photodynamic antimicrobial therapy.

Research Products

• [Journal Article] Bactericidal Activity and Mechanism of Photoirradiated Polyphenols against Gram-Positive and -Negative Bacteria. (2015)
  • Author(s): Nakamura K, Ishiyama K, Sheng H, Ikai H, Kanno T, Niwano Y.
  • Journal Title: J Agric Food Chem. Volume: 印刷中 Issue: 35 Pages: 7707-7713
  • DOI: 10.1021/jf5058588
  • Peer Reviewed
• [Journal Article] Microbicidal activity of artificially generated hydroxyl radicals (2015)
  • Author(s): Sheng H, Nakamura K ,Kanno T, Sasak K, Niwano Y
  • Journal Title: Interface Oral Health Science 2014 Volume: - Pages: 203-216
  • Open Access
• [Journal Article] Postantibiotic effect of disinfection treatment by photolysis of hydrogen peroxide. (2014)
  • Author(s): Odashima, Y., Nakamura, K., Ikai, H., Kanno, T., Meirelles, L., Sasaki, K., Niwano, Y.
  • Journal Title: Journal of Chemotherapy Volume: 26(2): Issue: 2 Pages: 92-100
  • DOI: 10.1179/1973947813y.0000000114
  • Peer Reviewed
• [Journal Article] Synergistic Effect of Proanthocyanidin on the Bactericidal Action of the Photolysis of H<SUB>2</SUB>O<SUB>2</SUB> (2013)
  • Author(s): Ikai, H., Nakamura, K., Kanno, T., Shirato. M., Meirelles, L., Sasaki, K., Niwano, Y
  • Journal Title: Biocontrol Science Volume: 18 Issue: 3 Pages: 137-141
  • DOI: 10.4265/bio.18.137
  • NAID: 10031202904
  • ISSN: 1342-4815, 1884-0205
  • Peer Reviewed
• [Journal Article] Influence of a new denture cleaning technique based on photolysis of H₂O₂ the mechanical properties and color change of acrylic denture base resin (2013)
  • Author(s): Nakahara T, Harada A, Yamada Y, Odashima Y, Nakamura K, Inagaki R, Kanno T, Sasaki K, Niwano Y
  • Journal Title: Dental Materials Journal Volume: 32 Issue: 4 Pages: 529-536
  • DOI: 10.4012/dmj.2013-047
  • NAID: 10031178633
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed
• [Journal Article] In vitro evaluation of the risk of inducing bacterial resistance to disinfection treatment with photolysis of hydrogen peroxide (2013)
  • Author(s): Ikai, H., Odashima, Y., Kanno, T., Nakamura, K., Shirato, M., Sasaki, K., Niwano, Y
  • Journal Title: PLoS ONE Volume: 8(11) Issue: 11 Pages: e81316-e81316
  • DOI: 10.1371/journal.pone.0081316
  • Peer Reviewed
• [Journal Article] Photo-irradiation of proanthocyanidin as a new disinfection technique via reactive oxygen species formation (2013)
  • Author(s): Nakamura K, Shirato M, Ikai H, Kanno T, Sasaki K, Kohno M, Niwano Y.
  • Journal Title: PLoS ONE Volume: 8 Issue: 3 Pages: e60053-e60053
  • DOI: 10.1371/journal.pone.0060053
  • Peer Reviewed
• [Journal Article] Bactericidal action of photogenerated singlet oxygen from photosensitizers used in plaque disclosing agents (2012)
  • Author(s): Ishiyama K, Nakamura K, Ikai H, Kanno T, Kohno M, Sasaki K, Niwano Y
  • Journal Title: PLoS ONE Volume: 7 Issue: 5 Pages: e37871-e37871
  • DOI: 10.1371/journal.pone.0037871
  • Peer Reviewed
• [Journal Article] Bactericidal action of photo-irradiated gallic acid via reactive oxygen species formation (2012)
  • Author(s): Nakamura K, Yamada Y, Ikai H, Kanno T, Sasaki K, Niwano Y.
  • Journal Title: Journal of Agricultural and Food Chemistry Volume: 60 Issue: 40 Pages: 10048-10054
  • DOI: 10.1021/jf303177p
  • Peer Reviewed
• [Presentation] 活性酸素 (2014)
  • Author(s): 中村圭祐、菅野太郎、猪飼紘代、庭野吉己
  • Organizer: 第41回日本防菌防黴学会年次大会
  • Place of Presentation: 品川（東京）
  • Year and Date: 2014-09-24 – 2014-09-25
  • Invited
• [Presentation] Comparison of bactericidal activity of photo-irradiated polyphenols (2014)
  • Author(s): Nakamura K, Ishiyama K, Ikai H, Kanno T, Niwano Y
  • Organizer: The XXVIIth International Conference on Polyphenols
  • Place of Presentation: 名古屋（愛知）
  • Year and Date: 2014-09-02 – 2014-09-06
• [Presentation] Bactericidal effect of photolysis of H2O2 in combination with sonolysis of water via hydroxyl radical generation (2014)
  • Author(s): Sheng H, Nakamura K ,Kanno T, Sasak Ki, Niwano Y
  • Organizer: The 5th international symposium for interface oral science in Sendai
  • Place of Presentation: 宮城
• [Presentation] Acceleration of proliferative response of 3T3-L1 mouse fibroblasts and human gingival fibroblasts by short-term exposure to proanthocyanidin (2014)
  • Author(s): Kurauchi M, Nakamura K, Kanno T, Shirato M, Sasaki K, Niwano Y
  • Organizer: The 5th international symposium for interface oral science in Sendai
  • Place of Presentation: 宮城
• [Presentation] Bactericidal action of photogenerated singlet oxygen from photosensitizers used in plaque disclosing agents (2013)
  • Author(s): Ishiyama, K., Nakamura, K., Ikai, H., Kanno, T., Sasaki, K., Niwano, Y
  • Organizer: Innovative Research for Biosis-Abiosis Intelligent Interface Summer Seminar 2013
  • Place of Presentation: 宮城
• [Presentation] Advantages of new disinfection treatment utilizing photolysis of hydrogen peroxide - Low risk of inducing bacterial resistance and postantibiotic effect (2013)
  • Author(s): Ikai, H., Odashima, Y., Nakamura, K., Shirato, M., Kanno, T., Sasaki, K., Niwano, Y
  • Organizer: Innovative Research for Biosis-Abiosis Intelligent Interface Summer Seminar 2013
  • Place of Presentation: 宮城
• [Presentation] 電気分解で生成された酸性電解水中に存在する水酸化ラジカルは殺菌活性に寄与するか？ (2013)
  • Author(s): 目代貴之、中村圭祐、菅野太郎、庭野吉己
  • Organizer: 第40回日本防菌防黴学会年次大会
  • Place of Presentation: 大阪
• [Presentation] 過酸化水素光分解殺菌法の短時間処理が残存細菌に及ぼす増殖抑制効果 (2013)
  • Author(s): 小田島優、中村圭祐、猪飼紘代、菅野太郎、佐々木啓一、庭野吉己
  • Organizer: 第40回日本防菌防黴学会年次大会
  • Place of Presentation: 大阪
• [Presentation] 過酸化水素光分解殺菌法の耐性誘導試験 (2013)
  • Author(s): 猪飼紘代 、中村圭祐、小田島優、白土翠、菅野太郎、庭野吉己
  • Organizer: 第40回日本防菌防黴学会年次大会
  • Place of Presentation: 大阪
• [Presentation] 各種ポリフェノールに対する青色可視光照射で得られる殺菌効果の比較検討 (2013)
  • Author(s): 中村圭祐、石山希里香、猪飼紘代、菅野太郎、庭野吉己
  • Organizer: 第40回日本防菌防黴学会年次大会
  • Place of Presentation: 大阪
• [Presentation] 各種光感受性プラーク染色剤から生成する一重項酸素による殺菌効果の比較検討 (2012)
  • Author(s): 石山希里香，中村圭祐，猪飼紘代，菅野太郎，佐々木啓一，庭野吉己
  • Organizer: 第64回日本酸化ストレス学会学術集会
  • Place of Presentation: 徳島
• [Presentation] 歯垢染色剤を用いた光線力学殺菌療法の比較検討 (2012)
  • Author(s): 石山希里香，中村圭祐，猪飼紘代，菅野太郎，佐々木啓一，庭野吉己
  • Organizer: 第39回日本防菌防黴学会年次大会
  • Place of Presentation: 東京
• [Presentation] ポリフェノールへの青色可視光照射により生成される活性酸素を応用した新規殺菌技術 (2012)
  • Author(s): 中村圭祐、山田康友、猪飼紘代、菅野太郎、佐々木啓一、庭野吉己
  • Organizer: 第39回日本防菌防黴学会年次大会
  • Place of Presentation: 東京
• [Presentation] プラーク染色剤を用いた新しい光線力学殺菌療法の基礎的検討 (2012)
  • Author(s): 石山希里香、中村圭祐、猪飼紘代、菅野太郎、佐々木啓一、庭野吉己
  • Organizer: 第22回日本歯科医学会総会
  • Place of Presentation: 大阪