| Project/Area Number |
24792006
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 温熱療法 / 分子標的薬 / 併用療法 / 口腔癌 / 口腔扁平上皮癌 |
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| Research Abstract |
Interleukin-13 receptor alpha 2 chain (IL-13Ra2), a unique tumor-associated antigen, is a promising target for cancer immunotherapy. IL13-PE, a targeted cytotoxin composed of IL-13 and mutated Pseudomonas exotoxin, induces specific killing of IL-13Ra2 positive tumor cells. Our objective is whether hyperthermia treatment of oral squamous cell carcinoma (OSCC) can modulate the expression of IL-13Ra2 and increase their sensitivity to IL13-PE.
OSCC cell lines, HSC-3 and SCC-25 cells were heated with 43 degree for 1 hour. The proliferation of heat-stressed OSCC cells showed more than 50% growth inhibition compared to control cells. IL-13Ra2 was up-regulated after heating of OSCC cells. Protein synthesis inhibition assay with IL13-PE showed that heat-stressed OSCC cells decreased the number of IC50 compared to that of without heated OSCC cells. The percentages of apoptotic cells increased in OSCC cells treated with IL13-PE after hyperthermia compared with controls.
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