Study of the relationship between mechanism of transport of intracellular cisplatin and cisplatin resistance

• Project/Area Number: 24792007
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Kyushu Dental College
• Principal Investigator: MATSUMOTO Shinobu 九州歯科大学, 歯学部, 助教 (20514996)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: シスプラチン / トランスポーター / 薬剤耐性

Outline of Final Research Achievements

To elucidate the mechanism of cisplatin, the distributions of intracellular platinum were searched using a transmission electron microscope (TEM) and a scanning electron microscope (SEM). In individual cell lines which varied their sensitivity to cisplatin, by the analysis using the TEM, much chromatin stained with Pt was observed, and condensed. These findings suggested that cisplatin was incorporated into nuclear and bound to the DNA, thereby agglutinating chromatin. It was also indicated that platinum plays the role as a stain of an electron staining. A peak of platinum was detected by mapping examination by the SEM, and revealed a localization of cisplatin in the cells.