The effects of lysophosphatidic acid on osteoclast activity.

• Project/Area Number: 24792009
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Nihon University
• Principal Investigator: TANABE Natsuko 日本大学, 歯学部, 准教授 (10409097)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: メカニカルストレス / ATP / P2X7 / 骨芽細胞 / 破骨細胞

Outline of Final Research Achievements

LPA stimulated the expression of IL-1beta in osteoclast precursor cell line RAW 264.7. LPA-induced IL-1beta also enhanced osteoclast survival.
Mechanical stimuli such as Low-intensity pulsed ultrasounds (LIPUS) and tension force (TF) induced extracellular ATP release. Mechanical stimuli-induced ATP activated P2X7 receptor increased extracellular matrix proteins and bone formation. However, LIPUS enhanced bone formation without the increases of ALPase activity.

Research Products

• [Journal Article] Tension force-induced ATP promotes osteogenesis through P2X7 receptor in osteoblasts. (2015)
  • Author(s): Kariya T, Tanabe N, Shionome C, Kawato T, Zhao N, Maeno M, Suzuki N, Shimizu N
  • Journal Title: Journal of Cellular Biochemistry Volume: 116 Issue: 1 Pages: 12-21
  • DOI: 10.1002/jcb.24863
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3E1 cells. (2015)
  • Author(s): Manaka S, Tanabe N, Kariya T, Naito M, Takayama T, Nagao M, Liu D, Ito K, Maeno M, Suzuki N, Miyazaki M
  • Journal Title: FEBS Letters Volume: 589 Issue: 3 Pages: 310-318
  • DOI: 10.1016/j.febslet.2014.12.013
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Subcellular elevation of cytosolic free calcium is required for osteoclast migration. (2014)
  • Author(s): Wheal BD, Beach RJ, Tanabe N, Dixon SJ, Sims SM
  • Journal Title: J Bone Miner Res. Volume: 29 Issue: 3 Pages: 725-34
  • DOI: 10.1002/jbmr.2068
  • Peer Reviewed
• [Journal Article] Effects of Isoform-Selective Phosphatidylinositol 3-Kinase Inhibitors on Osteoclasts: ACTIONS ON CYTOSKELETAL ORGANIZATION, SURVIVAL AND RESORPTION. (2013)
  • Author(s): Shugg RP, Thomson A, Tanabe N, Kashishian A, Steiner BH, Puri KD, Pereverzev A, Lannutti BJ, Jirik FR, Dixon SJ, Sims SM
  • Journal Title: J Biol Chem. Volume: 288 Issue: 49 Pages: 35346-57
  • DOI: 10.1074/jbc.m113.507525
  • Peer Reviewed
• [Journal Article] Nicotine affects bone resorption and suppresses the expression of cathepsin K, MMP-9 and vacuolar-type H+-ATPase d2 and actin organization in osteoclasts (2013)
  • Author(s): Tanaka H, Tanabe N, Kawato T, Nakai K, Kariya T, Matsumoto S, Zhao N, Motohashi M, Maeno M
  • Journal Title: PLoS One Volume: 8 Issue: 3 Pages: e59402-e59402
  • DOI: 10.1371/journal.pone.0059402
  • Peer Reviewed
• [Journal Article] Vaspin attenuates RANKL-induced osteoclast formation in RAW264.7 cells. (2013)
  • Author(s): Kamio N, Kawato T, Tanabe N
  • Journal Title: Connect Tissue Res. Volume: 54(2) Pages: 147-52
  • Peer Reviewed
• [Presentation] Low-intensity pulsed ultrasound-induced ATP increases osteogenesis via P2X7 in MC3T3E1 cells. (2014)
  • Author(s): Soichiro Manaka, Natsuko Tanabe , Tadahiro Takayama, Mayu Nagao , Yasuo Tsuji , Kenji Okuno , Naoyuki Sugano , Naoto Suzuki, Koichi Ito, Bunnai Ogiso
  • Organizer: 第100回AAPアメリカ歯周病学会
  • Place of Presentation: アメリカ合衆国・サンフランシスコ
  • Year and Date: 2014-09-18 – 2014-09-22
• [Presentation] Tension force-induced ATP promotes osteoblast differentiation-related transcription factors and osteogenesis through P2X7 receptor in osteoblasts (2014)
  • Author(s): Natsuko Tanabe, Taro Kariya, Chieko Shionome, Takayuki Kawato, Masao Maeno, Noriyoshi Shimizu, Naoto Suzuki
  • Organizer: American Society of Bone and Mineral Research 2014 Annual Meeting
  • Place of Presentation: アメリカ合衆国・ヒューストン
  • Year and Date: 2014-09-14 – 2014-09-16
• [Presentation] Nicotine reduces bone resorption due to the suppression of cathepthin K, MMP-9 and vacuolar-type-ATPase D2 production and actin organization in osteoclasts.
  • Author(s): Tanabe, N
  • Organizer: 第20回IFCC-EFLMK
  • Place of Presentation: イタリア，ミラノコンベンションセンター
• [Presentation] Tention force-induced ATP promoters osteogenesis via the P2X7 in osteoblasts.
  • Author(s): Tanabe N
  • Organizer: PER/IADR congress & Exhibition
  • Place of Presentation: Finlandia Hall, Helsinki, Finland
• [Presentation] Angiotensin II induces osteoblast production of collagenase and stromelysin.
  • Author(s): Nakai K
  • Organizer: PER/IADR congress & Exhibition
  • Place of Presentation: Finlandia Hall, Helsinki, Finland
• [Presentation] Interleukin-17A induces extracellular matrix protein expression in osteoblastic ROS17/2.8 cells.
  • Author(s): Maeno M
  • Organizer: PER/IADR congress & Exhibition
  • Place of Presentation: Finlandia Hall, Helsinki, Finland
• [Presentation] ニコチンがRAW264.7細胞の破骨細胞への分化と無機質溶解に及ぼす影響
  • Author(s): 田中秀樹
  • Organizer: 第61回日本口腔衛生学会・総会
  • Place of Presentation: 神奈川，横須賀市，神奈川歯科大学
• [Presentation] オステオポンチンはNFATc1を活性化して破骨細胞の生存を促進する.
  • Author(s): 田邉奈津子
  • Organizer: 第61回日本口腔衛生学会・総会
  • Place of Presentation: 神奈川，横須賀市，神奈川歯科大学
• [Presentation] ニコチンが破骨細胞の分化と無機質溶解に及ぼす影響について.
  • Author(s): 田中秀樹
  • Organizer: 日本歯科保存学会2012年度春季学術大会
  • Place of Presentation: 沖縄，宜野湾市，コンベンションセンター
• [Presentation] ニコチンが破骨細胞の分化と無機質溶解に及ぼす影響
  • Author(s): 田中秀樹
  • Organizer: 第21回硬組織再生生物学会学術大会
  • Place of Presentation: 愛知，名古屋市，愛知学院大学
• [Presentation] 骨芽細胞への伸展力負荷はP2X7受容体を介して石灰化物形成を促進する.
  • Author(s): 仮谷太良
  • Organizer: 第21回硬組織再生生物学会学術大会
  • Place of Presentation: 愛知，名古屋市，愛知学院大学