Enhancement of functional expression of osteoblastic and periosteal cells by gingival fibroblasts with paracrine effect of fibroblastic growth factors

• Project/Area Number: 24792114
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Prosthetic dentistry
• Research Institution: Tokyo Dental College
• Principal Investigator: YAMADA Masahiro 東京歯科大学, 歯学部, 講師 (90549982)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 骨再生 / 線維芽細胞増殖因子 / 歯肉結合組織 / 細胞間相互作用 / 歯肉線維芽細胞

Research Abstract

Gingival fibroblasts enhanced osteoblastic and periosteal proliferation and differentiation. The mechanism was involved in paracrine secretion of FGF-1 and -18 from GF and subsequent autocrine secresion of these growth factors from osteoblasts and periosteal cells.

Research Products

• [Journal Article] Early-stage osseointegration capability of a submicrofeatured titanium surface created by microroughening and anodic oxidation (2013)
  • Author(s): Yamada M, Ueno T, Minamikawa H, Ikeda T, Nakagawa K, Ogawa T
  • Journal Title: Clin Oral Implants Res Volume: 24(9) Pages: 991-1001
• [Journal Article] Success rate, healing time, and implant stability development of photofunctionalized dental implants (2013)
  • Author(s): Funato A, Yamada M, Ogawa T
  • Journal Title: Int J Oral Maxillofac Implants Volume: 28(5) Pages: 1261-71
• [Journal Article] N-acetyl cysteine as an osteogenesis-enhancing molecule for bone regeneration (2013)
  • Author(s): Yamada M, Tsukimura N, Ikeda T, Sugita Y, Att W, Kojima N, Kubo K, Ueno T, Sakurai K, Ogawa T
  • Journal Title: Biomaterials Volume: 34(26) Pages: 6147-56
• [Journal Article] A novel combined surgical approach to vertical alveolar ridge augmentation with titanium mesh, resorbable membrane, and rhPDGF-BB : a retrospective consecutive case series (2013)
  • Author(s): Funato A, Ishikawa T, Kitajima H, Yamada M, Moroi H
  • Journal Title: Int J Periodontics Restorative Dent Volume: 33(4) Pages: 159-66
• [Journal Article] Activation of osteoblastic differentiation through calcium supplement by beta-tricalcium phosphate collagen composite leading to initial inner bone formation (2013)
  • Author(s): 加藤英治, 山田将博, 櫻井 薫
  • Journal Title: 日本口腔インプラント学会誌 Volume: 26(3) Pages: 405-424
  • NAID: 130004631892
• [Journal Article] Anti-oxidant amino acid derivative as a multifunctional molecule for bone regeneration (2013)
  • Author(s): 山田将博, 小川隆広, 櫻井 薫
  • Journal Title: 日本補綴歯科学会誌 Volume: 5(4) Pages: 411-413
  • NAID: 10031202888
• [Journal Article] Early-stage osseointegration capability of a submicrofeatured titanium surface created by microroughening and anodic oxidation (2013)
  • Author(s): Yamada M, Ueno T, Minamikawa H, Ikeda T, Nakagawa K, Ogawa T.
  • Journal Title: Clinical oral implant research Volume: 24(9) Issue: 9 Pages: 991-1001
  • DOI: 10.1111/j.1600-0501.2012.02507.x
  • Peer Reviewed
• [Journal Article] Effect of photofunctionalization on fluoride-treated nanofeatured titanium (2013)
  • Author(s): Ikeda T, Hagiwara Y, Hirota M, Tabuchi M, Yamada M, Sugita Y, Ogawa T.
  • Journal Title: J Biomater Appl. Volume: 28 Issue: 8 Pages: 1200-1212
  • DOI: 10.1177/0885328213501566
  • Peer Reviewed
• [Journal Article] A novel combined surgical approach to vertical alveolar ridge augmentation with titanium mesh, resorbable membrane, and rhPDGF-BB: a retrospective consecutive case series. (2013)
  • Author(s): Funato A, Ishikawa T, Kitajima H, Yamada M, Moroi H.
  • Journal Title: Int J Periodontics Restorative Dent. Volume: 33 Issue: 4 Pages: 159-166
  • DOI: 10.11607/prd.1460
  • Peer Reviewed
• [Journal Article] Success rate, healing time, and implant stability development of photofunctionalized dental implants. (2013)
  • Author(s): Funato A, Yamada M, Ogawa T.
  • Journal Title: Int J Oral Maxillofac Implants. Volume: 28 Issue: 5 Pages: 1261-1271
  • DOI: 10.11607/jomi.3263
  • Peer Reviewed
• [Journal Article] N-acetyl cysteine as an osteogenesis-anhancing molecule for bone regeneration (2013)
  • Author(s): Yamada M, Tsukimura N, Ikeda T, Sugita Y, Att W, Kojima N, Kubo K, Ueno T, Sakurai K, Ogawa T
  • Journal Title: Biomaterials Volume: 34(26) Issue: 26 Pages: 6147-6156
  • DOI: 10.1016/j.biomaterials.2013.04.064
  • Peer Reviewed
• [Journal Article] Activation of osteoblastic differentiation through calcium supplement by beta-tricalcium phosphate collagen composite leading to initial inner bone formation. (2013)
  • Author(s): 加藤英治, 山田将博, 櫻井 薫
  • Journal Title: 日本口腔インプラント学会誌 Volume: 26 Pages: 405-417
  • NAID: 130004631892
  • Peer Reviewed
• [Journal Article] 特集：日本補綴歯科学会第121 回学術大会イブニングセッション2 臨床イノベーションのための若手研究者の挑戦：補綴治療のための検査法の新たな展開 骨再生における多機能性分子としての抗酸化アミノ酸誘導体 (2013)
  • Author(s): 山田将博，小川 隆広，櫻井 薫
  • Journal Title: 日補綴会誌 Volume: 5 Pages: 411-413
• [Journal Article] Bone integration capability of nanopolymorphic crystalline hydroxyapatite coated on titanium implants (2012)
  • Author(s): Yamada M, Ueno T, Tsukimura N, Ikeda T, Nakagawa K, Hori N, Suzuki T, Ogawa T
  • Journal Title: Int J Nanomedicine Volume: 7 Pages: 859-73
• [Journal Article] N-acetyl Cysteine Improves Affinity of Beta-Tricalcium Phosphate Granules for Cultured Osteoblast-like Cells (2012)
  • Author(s): Yamada, M., Minamikawa, H., Ueno, T., Sakurai, K., Ogawa, T
  • Journal Title: J Biomater Appl Volume: 27(1) Pages: 37-36
• [Journal Article] Biodegradation Property of Beta-Tricalcium Phosphate-Collagen Composite in Accordance with Bone Formation: A Comparative Study with Bio-Oss Collagen® in a Rat Critical-Size Defect Model. (2012)
  • Author(s): Kato, E., Lemler, J., Sakurai, K. and Yamada, M.
  • Journal Title: Clin Implant Dent Relat Res Volume: - Issue: 2 Pages: 202-211
  • DOI: 10.1111/j.1708-8208.2012.00467.x
  • Peer Reviewed
• [Journal Article] N-acetyl Cysteine Improves Affinity of Beta-Tricalcium Phosphate Granules for Cultured Osteoblast-like Cells (2012)
  • Author(s): Yamada M, Minamikawa H, Ueno T, Sakurai K, Ogawa T
  • Journal Title: J Biomater Appl. Volume: (In press) Issue: 1 Pages: 27-36
  • DOI: 10.1177/0885328210383598
  • Peer Reviewed
• [Journal Article] Bone integration capability of nanopolymorphic crystalline hydroxyapatite coated on titanium implants. (2012)
  • Author(s): Yamada M, Ueno T, Tsukimura N, Ikeda T, Nakagawa K, Hori N, Suzuki T, Ogawa T.
  • Journal Title: Int J Nanomedicine Volume: 7 Pages: 859-873
  • DOI: 10.2147/ijn.s28082
  • Peer Reviewed
• [Presentation] Cytofunctional preconditioning for auto-cell transplantation to enhance bone regeneration using antioxidant amino acid derivative (2014)
  • Author(s): Yamada, M., Ogawa, T. and Sakurai K.
  • Organizer: the 29th Anniversary Meeting of Academy of Osseointegration
  • Place of Presentation: Seattle, USA
• [Presentation] Establishment of connective tissue integration with alkali-heat treated titanium surface (2014)
  • Author(s): Kato, E., Yamada, M. and Sakurai K.
  • Organizer: the 29th Anniversary Meeting of Academy of Osseointegration
  • Place of Presentation: Seattle, USA
• [Presentation] Establishment of connective tissue integration with alkali-heat treated titanium surface (2013)
  • Author(s): Kato, E., Yamada, M. and Sakurai K
  • Organizer: 29th Annual Meeting of Academy of Osseointegration
  • Place of Presentation: Seattle, USA
  • Year and Date: 2013-03-06
• [Presentation] N-アセチル-L-システインの細胞内吸収によるヒト気管支上皮細胞の細菌性炎症反応の抑制 (2013)
  • Author(s): 小泉ちあき，山田将博，上田貴之，石崎 憲，玉井久貴，櫻井 薫
  • Organizer: 平成25年度(公社)日本補綴歯科学会東京支部学術大会
  • Place of Presentation: 東京
• [Presentation] Strong dermal collagen binding capability of alkali-heat-treated titanium surface with substantial nano-tuft-like structure (2013)
  • Author(s): Yamada M, Kato E, Sakurai K.
  • Organizer: International College of Prosthodontists 15th Biennial Meeting
  • Place of Presentation: Torino, Italy
• [Presentation] アルカリ熱処理によるナノ毛羽立ち状チタン表面のヒト真皮コラーゲン付着強度の向上 (2013)
  • Author(s): 山田将博，加藤英治，櫻井 薫
  • Organizer: 日本口腔インプラント学会第43回学術大会
  • Place of Presentation: 福岡
• [Presentation] 毛羽立ち状ナノ構造体を介したアルカリ熱処理チタン表面における歯肉コラーゲン線維の機械的嵌 合の確立と付着強度の向上 (2013)
  • Author(s): 加藤英治，山田将博，櫻井 薫
  • Organizer: 日本口腔インプラント学会第43回学術大会
  • Place of Presentation: 福岡
• [Presentation] N-アセチルシステインを用いたヒト気管支上皮細胞の粘液産生増加の予防 (2013)
  • Author(s): 小泉ちあき, 山田将博, 上田貴之, 石崎憲, 櫻井薫
  • Organizer: 第295回東京歯科大学学会
  • Place of Presentation: 千葉
• [Presentation] A Development of the Most Suitable Titanium Topography for the Transmucosal Abutment Module and the Real Soft Tissue Integration (2013)
  • Author(s): Kato, E., Lember, J, R., Yamada, M., Sakurai, K.
  • Organizer: Academy of Osseoingration 28th Annual Meeting
  • Place of Presentation: Florida, USA
• [Presentation] Alkali-heat-treated Titanium Surface Enhances Collagen Deposition and Binding Strength (2013)
  • Author(s): Yamada, M., Kato, E., Sakurai, K.
  • Organizer: 91th General Session of the IADR
  • Place of Presentation: Seattle, USA
• [Presentation] N-acetyl Cysteine Alleviates Damage of Epithelial Monolayer from PMMA Extracts (2013)
  • Author(s): Nishimiya H, Yamada M, Ueda T and Sakurai K.
  • Organizer: 91th General Session of the IADR
  • Place of Presentation: Seattle, USA
• [Presentation] Cytofunctional preconditioning for auto-cell transplantation to enhance bone regeneration using antioxidant amino acid derivative (2012)
  • Author(s): Yamada, M., Ogawa, T. and Sakurai K
  • Organizer: 29th Anniversary Meeting of Academy of Osseointegration
  • Place of Presentation: Seattle, USA
  • Year and Date: 2012-03-06
• [Presentation] Staphylococcal attachment reduces on UVC-irradiated titanium by changing surface properties (2012)
  • Author(s): Yamada, Y., Yamada, M., Ueda, T., Sakurai, K.
  • Organizer: 36th Annual Congress of the European Prosthodontic Association
  • Place of Presentation: Rotterdam, Netherlands
• [Presentation] UVC dose-dependent alteration in osteobiological and physicochemical properties of titanium (2012)
  • Author(s): Uchiyama, H., Yamada, M., Ishizaki, K., Sakurai, K.
  • Organizer: 36th Annual Congress of the European Prosthodontic Association
  • Place of Presentation: Rotterdam, Netherlands
• [Presentation] Alkali-heat-treated Titanium Surface Enhances Fibroblastic Collagen Deposition and Binding Strength
  • Author(s): Yamada M, Kato E and Sakurai K
  • Organizer: the 90th General Session and Exhibition of the IADR
  • Place of Presentation: Seattle, USA
• [Presentation] N-acetyl Cysteine Alleviates Damage of Epithelial Monolayer from PMMA Extracts
  • Author(s): Nishimiya H, Yamada M, Ueda T and Sakurai K
  • Organizer: the 90th General Session and Exhibition of the IADR
  • Place of Presentation: Seattle, USA
• [Remarks] 東京歯科大学HRC プロジェクト研究HRC 第8プロジェクト・免疫機能・トランスレーショナル研究グループホームページ
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• [Remarks] 東京歯科大学有床義歯補綴学講座ホームページ
  • URL: http://www.tdc.ac.jp/dept/rpg/
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