A search and evaluation of the peptides that control the connective tissue growth factor for the prevention and the treatment of osteoarthritis.

• Project/Area Number: 24792142
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Okayama University
• Principal Investigator: OIDA Yasutaka 岡山大学, 大学病院, 医員 (50625720)
• Research Collaborator: KUBOKI Takuo 岡山大学, 大学院・医歯薬学総合研究科インプラント再生補綴学分野, 教授 ; SONOYAMA Wataru 岡山大学, 大学院・医歯薬学総合研究科インプラント再生補綴学分野, 講師 ; ONO Mitsuaki 岡山大学, 大学院・医歯薬学総合研究科インプラント再生補綴学分野, 助教 ; ハラ エミリオ サトシ 岡山大学, 大学院・医歯薬学総合研究科インプラント再生補綴学分野, 助教 ; SHINKAWA Shigehiko 岡山大学, 大学院・医歯薬学総合研究科インプラント再生補綴学分野, 医員 ; NAKAJIMA Ryu 岡山大学, 大学院・医歯薬学総合研究科インプラント再生補綴学分野, 大学院生
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ハルミン / CCN2 / β-カルボリン / 軟骨細胞 / 軟骨再生 / Harmine / 変形性関節症 / 軟骨分化 / 結合組織成長因子 CTGF

Research Abstract

CCN2 / connective tissue growth factor (CTGF) has been reported to have essential role in cartilage development, chondrocyte proliferation and differentiation as well as regulation of the extracellular matrix metabolism. This study screened a compound library and identified harmine as a novel inducer of CCN2. This finding indicates harmine as a potential drug for prevention and / or repair of cartilage degradation.

Research Products

• [Journal Article] Novel chondrogenic and chondroprotective effects of the natural compound harmine. (2013)
  • Author(s): Hara, E. S., Ono, M., Kubota, S., Sonoyama, W., Oida, Y., Hattori, T., Nishida, T., Furumatsu, T., Ozaki, T., Takigawa, M. and Kuboki, T
  • Journal Title: Biochimie Volume: 95 Issue: 2 Pages: 347-381
  • DOI: 10.1016/j.biochi.2012.10.016
  • Peer Reviewed
• [Presentation] Enhanced TGFβ3-induced chondrogenesis of ATDC5 cells by fluocinolone acetonide. (2012)
  • Author(s): Hara ES, Ono M, Kubota S, Sonoyama W, Fujisawa T, Takigawa M, Kuboki T.
  • Organizer: 第30 日本骨代謝学会
  • Place of Presentation: 大阪
• [Presentation] The CCN2-inducer Harmine promotes chondrogenesis and protection against TNFα-induced inflammation (2011)
  • Author(s): Hara ES, Ono M, Kubota S, Sonoyama W, Oida Y, Hattori T, Nishida T, Furumatsu T, Ozaki T, Takigawa M, Kuboki T
  • Organizer: 第59 国際歯科研究学会日本部会 (JADR) 総会・学術大会- Hatton award competition
  • Place of Presentation: Hiroshima, Japan
  • Year and Date: 2011-10-08
• [Presentation] The CCN2-inducer Harmine promotes chondrogenesis and protection against TNFα-induced inflammation (2011)
  • Author(s): Hara ES, Ono M, Kubota S, Sonoyama W, Oida Y, Hattori T, Nishida T, Furumatsu T, Ozaki T, Takigawa M, Kuboki T
  • Organizer: 第59国際歯科研究学会日本部会 (JADR) 総会・学術大会
  • Place of Presentation: Hiroshima, Japan
  • Year and Date: 2011-10-08
• [Presentation] Harmine, an inducer of CCN2/CTGF, promotes chondrogenesis and rescues TNF-α-induced downregulation of cartilage markers (2011)
  • Author(s): Hara ES, Ono M, Kubota S, Sonoyama W, Hattori T, Nishida T, Takigawa M, Kuboki T
  • Organizer: 第2骨バイオサイエンス研究会
  • Place of Presentation: Okayama, Japan
  • Year and Date: 2011-08-28
• [Presentation] Harmine, an inducer of CCN2/CTGF, promotes chondrogenesis and protection against TNF-α-induced inflammatory condition (2011)
  • Author(s): Hara ES, Ono M, Kubota S, Sonoyama W, Hattori T, Nishida T, Furumatsu T, Ozaki T, Takigawa M, Kuboki T
  • Organizer: 第4日本CCNファミリー研究会
  • Place of Presentation: Okayama, Japan
  • Year and Date: 2011-08-28
• [Presentation] Harmine, an inducer of CCN2/CTGF, promotes chondrogenesis and suppresses TNF-α-induced inflammatory response (2011)
  • Author(s): Hara ES, Ono M, Kubota S, Sonoyama W, Hattori T, Takigawa M, Kuboki T
  • Organizer: 第29日本骨代謝学会
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2011-07-29
• [Presentation] Novel chondroprotective and anti-inflammatory effects of the natural compound harmine (2011)
  • Author(s): Hara ES, Ono M, Kubota S, Sonoyama W, Hattori T, Takigawa M, Kuboki T
  • Organizer: 第24日本軟骨代謝学会
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 2011-03-04