Study of hyperthermic sensitization as a target of DNA repair pathways in the oral cancer cells
| Project/Area Number |
24792244
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Nara Medical University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 臨床腫瘍学 / 温熱 / DNA修復 / 相同組み換え修復 / 口腔がん細胞 / siRNA / BRCA2 / recombination assay |
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| Research Abstract |
We investigated the possibility that the inhibition of homologous recombination (HR) repair or non-homologous end joining (NHEJ) repair enhances the heat sensitivity in cancer cells.There was no difference in the heat sensitivities of Ku80 defective cells and the parental cells. But, BRCA2-mutated cells were sensitive to heat as compared with the parental cells. In human tongue cancer cells, BRCA2-siRNA transfected cells were more sensitive to heat than the negative control-siRNA transfected cells. Apoptotic bodies were increased more efficiently in the BRCA2-siRNA transfected cells than the negative control-siRNA transfected cells after heat. A G2/M phase arrest was observed in the parental cells, but not in BRCA2-mutated cells after heat. DSBs were decreased at 18 h in the parental cells as compared with them at 0.5 h after heat. In contrast, they were not decreased at all in the BRCA2-mutated cells.These results suggest enhancement of heat sensitivity by depression of HR repair.
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Report
(3 results)
Research Products
(11 results)
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[Journal Article] Depression of p53-independent Akt survival signals in human oral cancer cells bearing mutated p53 gene after exposure to high-LET radiation.2012
Author(s)
Yosuke Nakagawa,Akihisa Takahashi,Atsuhisa Kajihara,Nobuhiro Yamakawa,Yuichiro Imai,Ichiro Ota,Noritomo Okamoto,Eiichiro Mori,Taichi Noda,Yoshiya Furusawa,Tadaaki Kirita,Takeo Ohnishi
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 423 (4)
Issue: 4
Pages: 654-660
DOI
Related Report
Peer Reviewed
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