Examination of the medical therapy of the multiple drug for the cancer pain

• Project/Area Number: 24792247
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Kyushu Dental College
• Principal Investigator: NOZOMU Harano 九州歯科大学, 歯学部, 助教 (50423976)
• Co-Investigator(Renkei-kenkyūsha): ONO Kentarou 九州歯科大学, 歯学部, 准教授 (40316154)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 口腔顔面痛 / 癌性疼痛 / endothelin-1 / endothelin-3 / BQ-123 / BQ-788 / 口内炎 / ガン性疼痛 / cisplatin / 癌モデル / 癌性疼痛治療

Outline of Final Research Achievements

We investigated the expression of the endothelin receptors ETA and ETB and endothelin induced responses in rat TRG neurons. These results suggest that ETA-mediated TRPV1 hyperactivation via PKC activation and ETB-mediated Ca2+ mobilization occurs in different subsets of TRG neurons.
These endothelin-induced responses may contribute to the induction of orofacial pain.The ETB-mediated function in TRG neurons is a special feature in the trigeminal system because of no ETB expression in dorsal root ganglion neurons.

Research Products

• [Journal Article] Novel methods of applying direct chemical and mechanical stimulation to the oral mucosa for traditional behavioral pain assays in conscious rats (2015)
  • Author(s): Suzuro Hitomi, Kentaro Ono, Kanako Miyano, Yojiro Ota, Yasuhito Uezono, Motohiro Matoba, Sachiko Kuramitsu, Kiichiro Yamaguchi, Kou Matsuo, Yuji Seta, Nozomu Harano, Kiyotoshi Inenaga
  • Journal Title: Journal of Neuroscience Methods Volume: 239 Pages: 162-169
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Endothelin Receptor-mediated Responses in Trigeminal Ganglion Neurons (2013)
  • Author(s): Yamamoto M, Ono K, Hitomi S, Harano N, Sago T, Yoshida M, Nunomaki M, Shiiba S, Watanabe S, Nakanishi O, Inenaga K.
  • Journal Title: J Dental Res Volume: 92 Issue: 4 Pages: 335-339
  • DOI: 10.1177/0022034513478428
  • Peer Reviewed
• [Journal Article] Distinct time courses of microglial and astrocytic hyper-activation and the glial contribution to pain hypersensitivity in a facial cancer model (2012)
  • Author(s): Sago T, Ono K, Harano N, Furuta-Hidaka K, Hitomi S, Nunomaki M, at al.(11名中2番目)
  • Journal Title: Brain Res Volume: (in press) Pages: 70-80
  • DOI: 10.1016/j.brainres.2012.03.039
  • Peer Reviewed
• [Journal Article] A rat pain model of facial cancer (2012)
  • Author(s): Ono K, Harano N, Inenaga K, Nakanishi O
  • Journal Title: Methods Mol Biol Volume: 851 Pages: 149-157
  • Peer Reviewed
• [Presentation] ラット口内炎モデルにおける疼痛発症への抗がん薬の影響 (2015)
  • Author(s): K. Yamaguchi, S. Hitomi, K. Ono, N. Harano, T. Sago, S. Watanabe, and K. Inenaga
  • Organizer: 第92回日本生理学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Effects of 5-fluorouracil on stomatitis-induced pain in rats (2014)
  • Author(s): Kiichiro Yamaguchi, Nozomu Harano, Suzuro Hitomi, Kentaro Ono, Teppei Sago, Seiji Watanabe, Kiyotoshi Inenaga
  • Organizer: Neuroscience
  • Place of Presentation: Washington DC
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] ラットにおける口内炎誘発疼痛に対する5-fluorouracilの影響 (2014)
  • Author(s): 山口喜一郎, 人見涼露, 小野堅太郎, 原野 望, 左合徹平, 稲永清敏
  • Organizer: 第56回歯科基礎医学会学術大会・総会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2014-09-25 – 2014-09-27