New therapeutic target associated with epigenetic changes with epithelial-mesenchymal transition of oral cancer

• Project/Area Number: 24792255
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Showa University
• Principal Investigator: YOSHIBA Sayaka 昭和大学, 歯学部, 助教 (60555358)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 口腔扁平上皮癌 / 上皮間葉移行 / DNAメチル化 / 口腔癌 / エピジェネティクス

Outline of Final Research Achievements

We were able to induce the epithelial-mesenchymal transition and (EMT) by TGF-β stimulation in oral squamous cell carcinoma cells. It induces EMT by TGF-β stimulation, and the attenuation of E-cadherin, enhancement of N-cadherin could be confirmed. From exhaustive DNA methylation analysis, we identified GPR-37 as a gene that is induced methylation, and LOM2 and PALLD as genes that are induced hypomethylation by transfoemation change by EMT.

Research Products

• [Journal Article] A case of odontogenic infection involves infratemporal and temporal fossa due to apical periodontitis of upper left second molar (2015)
  • Author(s): Sayaka Yoshiba, Takaaki Kamatani, Tatsuo Shirota
  • Journal Title: Indian Journal of Dentistry Volume: 1 Pages: 1-1
  • Peer Reviewed / Open Access
• [Journal Article] A Case of Amniotic Band Sequence (2015)
  • Author(s): Sayaka Yoshiba, Tetsutaro Yamaguchi, Sumimasa Ohtsuka, Tatsuo Shirota
  • Journal Title: Case Reports in Clinical Medicine Volume: 4 Pages: 124-130
  • Peer Reviewed
• [Journal Article] Primary minor salivary gland salivary duct carcinoma at palate: A radiological investigation and review of the literature (2014)
  • Author(s): Sayaka Yoshiba, Takaaki Kamatani, Daisuke Soga, Sunao Shiogama, Tomohiko Kutsuna, Satoru Shintani
  • Journal Title: Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology Volume: 26 Pages: 356-358
  • Peer Reviewed
• [Journal Article] Aberrant expression of EZH2 is associated with pathological findings and P53 alteration (2013)
  • Author(s): Shiogama S, Yoshiba S, Soga D, Motohashi H, Shintani S
  • Journal Title: Anticancer Res. 2013 Oct;33(10):4309-17. Volume: 10 Pages: 4309-4317
• [Journal Article] microRNA expression profiles in oral squamous cell carcinoma (2013)
  • Author(s): Daisuke Soga, Sayaka Yoshiba, Sunao Shiogama, Hiroaki Miyazaki, Seiji Kondo, Satoru Shintani
  • Journal Title: Oncology Reports Volume: 30 Issue: 2 Pages: 579-583
  • DOI: 10.3892/or.2013.2488
  • Peer Reviewed
• [Journal Article] Primary minor salivary gland salivary duct carcinoma at palate: A radiological investigation and review of the literature (2013)
  • Author(s): Sayaka Yoshiba, Takaaki Kamatani, Daisuke Soga, Sunao Shiogama, Tomohiko Kutsuna, Satoru Shintani
  • Journal Title: Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology Volume: なし Issue: 3 Pages: 356-358
  • DOI: 10.1016/j.ajoms.2013.02.010
  • Peer Reviewed
• [Presentation] 口腔内転移を認めた乳腺悪性葉状肉腫の１例 (2015)
  • Author(s): 葭葉 清香、渡辺 仁資、代田 達夫
  • Organizer: 口腔腫瘍学会
  • Place of Presentation: 奈良県新公会堂
  • Year and Date: 2015-01-29 – 2015-01-30
• [Presentation] 昭和大学横浜市北部病院歯科・歯科口腔外科開設後３年間における患者の臨床統計学的観察 (2014)
  • Author(s): 葭葉清香、宮久保あや子、吉田祐子、木村有子、正司めい、渡辺一葉、鈴木麻衣子、渡辺仁資、代田達夫
  • Organizer: 日本口腔外科学会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2014-10-17 – 2014-10-19
• [Presentation] 重度骨格性不正咬合を合併した羊膜破裂シークエンスの1例 (2013)
  • Author(s): ○葭葉 清香、代田 達夫、山口 徹太郎、大塚 純生、槇 宏太郎、新谷 悟
  • Organizer: 第23回日本顎変形症学会総会・学術大会
  • Place of Presentation: 福岡
• [Presentation] 頬リンパ節転移を認めた口腔扁平上皮癌の2例 (2013)
  • Author(s): 葭葉 清香、鎌谷宇明、代田 達夫、吉濱㤗斗、新谷 悟
  • Organizer: 第31回 日本口腔腫瘍学会総会・学術大会
  • Place of Presentation: 東京
• [Presentation] Epigenomic profiling of oral cancer (2012)
  • Author(s): Sayaka Yoshiba, Genta Nagae, Atsushi Kaneda, Shingo Tsuji, Keisuke Matsusaka, Koichi Yagi, Takayuki Isagawa, Tetsuhiko Tachikawa, Satoru Shintani, Hiroyuki Aburatani
  • Organizer: AACR Annual Meeting 2012
  • Place of Presentation: アメリカ シカゴ
• [Presentation] 上下顎移動術を施行した顎変形症患者における顎顔面形態の術後変化 (2012)
  • Author(s): 葭葉 清香、代田 達夫、山口 徹太郎、新 真紀子、栗原 祐史、槇 宏太郎、新谷 悟
  • Organizer: 第22回 顎変形症学会
  • Place of Presentation: 福岡
• [Presentation] MRI画像データを用いて経時的に病態を解析した進行性半側顔面萎縮症の1症例 (2012)
  • Author(s): 葭葉 清香、代田 達夫、関 健次、倉林仁美、吉村 節、新谷 悟
  • Organizer: 第57回 日本口腔外科学会
  • Place of Presentation: 横浜
• [Book] 口腔癌を知る (2012)
  • Author(s): 新谷悟、葭葉清香
  • Total Pages: 160
  • Publisher: 第一歯科出版