Peripheral nervous systems for development of new therapy in abnormal orofacial pain

• Project/Area Number: 24792258
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Nihon University
• Principal Investigator: HONDA Kuniya 日本大学, 歯学部, ポスト・ドクトラル・フェロー (20548945)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 神経因性疼痛 / 痛覚過敏 / TRP channel / グルタミン酸受容体 / 代謝型グルタミン酸受容体 / TRPA1 / TRPV1 / PKC epsilon / 疼痛

Research Abstract

Peripheral tissue injury causes glutamate release from keratinocytes, resulting in hyperalgesia. We have reported that peripheral glutamate injection induces thermal hyperalgesia. However, it is still not understood the mechanisms underlying hyperalgesia following peripheral glutamate injection. To clarify the involvement of peripheral TRPA1, TRPV1 and PKC epsilon glutamate-induced hyperalgesia, we performed behavioral testing, immunohistochemistry and neuronal recording using glutamate injected rats. Present findings suggest that TRPA1 or TRPV1 activation through mGluR5 signaling via PKC epsilon is involved in facial thermal and mechanical hyperalgesia.

Research Products

• [Journal Article] Nitric oxide signaling contributes to ectopic orofacial neuropathic pain (2013)
  • Author(s): Sugiyama T, Shinoda M, Watase T, Honda K, Ito R, Kaji K, Urata K, Lee J, Ohara K, Takahashi O, Echizenya S, Iwata K
  • Journal Title: J Dent Res Volume: 92 Pages: 1113-1117
  • Peer Reviewed
• [Journal Article] Interaction of IL-1β and P2X3 Receptor in Pathologic Masseter Muscle Pain (2013)
  • Author(s): Noma N, Shinoda M, Honda K, Kiyomoto M, Dezawa K, Nakaya Y, Komiyama O, Imamura Y, Iwata K
  • Journal Title: J Dent Res Volume: 92 Pages: 456-460
  • Peer Reviewed
• [Journal Article] Involvement of ERK Phosphorylation of Trigeminal Spinal Subnucleus Caudalis Neurons in Thermal Hypersensitivity in Rats with Infraorbital Nerve Injury (2013)
  • Author(s): Suzuki I, Tsuboi Y, Shinoda M, Shibuta K, Honda K, Katagiri A, Kiyomoto M, Sessle BJ, Matsuura S, Ohara K, Urata K, Iwata K
  • Journal Title: PLoS One Volume: 8
  • Peer Reviewed
• [Journal Article] Mechanisms underlying ectopic persistent tooth-pulp pain following pulpal inflammation (2013)
  • Author(s): Matsuura S, Shimizu K, Shinoda M, Ohara K, Ogiso B, Honda K, Katagiri A, Sessle BJ, Urata K, Iwata K
  • Journal Title: PLoS One Volume: 8
  • Peer Reviewed
• [Journal Article] Metabotropic glutamate receptor 5 contributes to inflammatory tongue pain via extracellular signal-regulated kinase signaling in the trigeminal spinal subnucleus caudalis and upper cervical spinal cord (2012)
  • Author(s): Liu MG, Matsuura S, Shinoda M, Honda K, Suzuki I, Shibuta K, Tamagawa T, Katagiri A, Kiyomoto M, Ohara K, Furukawa A, Urata K, Iwata K
  • Journal Title: J Neuroinflammation Volume: 27 Pages: 258-258
  • Peer Reviewed
• [Journal Article] P2X3 receptor mediates ectopic mechanical allodynia with inflamed lower lip in mice (2012)
  • Author(s): Yasuda M, Shinoda M, Kiyomoto M, Honda K, Suzuki A, Tamagawa T, Kaji K, Kimoto S, Iwata K
  • Journal Title: Neurosci Lett Volume: 528 Pages: 67-72
  • Peer Reviewed
• [Journal Article] Involvement of AMPA Receptor GluR2 and GluR3 Trafficking in Trigeminal Spinal Subnucleus Caudalis and C1/C2 Neurons in Acute-Facial Inflammatory Pain (2012)
  • Author(s): Miyamoto M, Tsuboi Y, Honda K, Kobayashi M, Takamiya K, Huganir RL, Kondo M, Shinoda M, Sessle BJ, Katagiri A, Kita D, Suzuki I, Oi Y, Iwata K
  • Journal Title: PLoS One Volume: 7
  • Peer Reviewed
• [Journal Article] Organization of hyperactive microglial cells in trigeminal spinal subnucleus caudalis and upper cervical spinal cord associated with orofacial neuropathic pain (2012)
  • Author(s): Shibuta K, Suzuki I, Shinoda M, Tsuboi Y, Honda K, Shimizu N, Sessle BJ, Iwata K
  • Journal Title: Brain Res Volume: 1451 Pages: 74-86
  • Peer Reviewed
• [Journal Article] Involvement of AMPA Receptor GluR2 and GluR3 Trafficking in Trigeminal Spinal Subnucleus Caudalis and C1/C2 Neurons in Acute-Facial Inflammatory Pain. (2012)
  • Author(s): Miyamoto, M.
  • Journal Title: PLoS One Volume: 7 Issue: 8 Pages: e44055-e44055
  • DOI: 10.1371/journal.pone.0044055
  • Peer Reviewed / Open Access
• [Journal Article] Metabotropic glutamate receptor 5 contributes to inflammatory tongue pain via extracellular signal-regulated kinase signaling in the trigeminal subnucleus caudalis and upper cervical spinal cord (2012)
  • Author(s): Ming-Gang Liu, Shingo Matsuura,
  • Journal Title: J Neuroinflammation Volume: 9 Issue: 1 Pages: 258-258
  • DOI: 10.1186/1742-2094-9-258
  • Peer Reviewed
• [Journal Article] 咀嚼筋群における筋筋膜痛症候群に起因した非歯原性歯痛と歯内疾患を併発した一例 (2012)
  • Author(s): 清水康平,佐藤隆夫,羽鳥啓介,小林千明,柴崎郁恵,重森葉子,加藤岳詩,橋本一浩,佐藤洋子,澤田朋美,小木曽文内
  • Journal Title: 日本歯科保存学雑誌 Volume: 55巻6号 Issue: 1 Pages: 424-431
  • DOI: 10.1371/journal.pone.0052840
  • Peer Reviewed
• [Presentation] 末梢の代謝型グルタミン酸受容体活性化により誘発される痛覚過敏に対するTRP channel の関与 (2014)
  • Author(s): 本田訓也, 岩田幸一
  • Organizer: 第91回日本生理学会
  • Place of Presentation: 鹿児島
  • Year and Date: 2014-03-18
• [Presentation] 末梢の代謝型グルタミン酸受容体活性化により誘発される痛覚過敏に対するTRP channnelの関与 (2014)
  • Author(s): 本田 訓也、岩田 幸一
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学 郡元キャンパス 鹿児島
• [Presentation] Activation of peripheral mGluR5 contributes thermal and mechanical hyperalgesia via TRPA1 and TRPV1 (2013)
  • Author(s): Kuniya Honda, Masamichi Shinoda and Koichi Iwata
  • Organizer: 8th Congress of the Europian Federation of IASP Chapters
  • Place of Presentation: Florence, Italy
  • Year and Date: 2013-10-11
• [Presentation] Peripheral glutamate receptors contribute cold hyperalgesia via TRPA1 mechanisms (2013)
  • Author(s): Kuniya Honda, Eriko Tachi, Kaori Kaji, Takaaki Tamagawa, Masamichi Shinod, Koichi Iwata
  • Organizer: 4th International Congress on Neuropathic Pain
  • Place of Presentation: Metro Toronto Convention Centre, Canada
• [Presentation] Activation of peripheral mGluR5 contributes thermal and mechanical hyperalgesia via TRPA1 and TRPV1 (2013)
  • Author(s): Kuniya Honda, Masamichi Shinoda, Koichi Iwata
  • Organizer: 8th Congress of the European Federation of IASP Chapters
  • Place of Presentation: Firenze Fiera S.p.A. Congress & Exhibition Center, Italy
• [Presentation] Peripheral glutamate receptors contribute cold hyperalgesia via TRPA1 mechanisms (2012)
  • Author(s): Kuniya Honda, Eriko Tachi, Kaori Kaji, Takaaki Tamagawa, Masamichi Shinoda and Koichi Iwata
  • Organizer: Neuroscience
  • Place of Presentation: New Orleans
  • Year and Date: 2012-10-15
• [Presentation] Peripheral NMDA receptor contributes cold hyperalgesia via TRPA1 mechanisms
  • Author(s): Kuniya Honda
  • Organizer: 8ｔｈ FENS forum of neuroscience
  • Place of Presentation: Ｂａｒｃｅｌｏｎａ, Spain
• [Presentation] Peripheral glutamate receptors contribute cold hyperalgesia via TRPA1 mechanisms
  • Author(s): Kuniya Honda
  • Organizer: Neurosceince 2012
  • Place of Presentation: New Orleans, United States
• [Book] 骨痛の基本的メカニズムがん骨転移のバイオロジーとマネージメント (2012)
  • Author(s): 岩田幸一, 本田訓也, 篠田雅路
  • Publisher: 医薬ジャーナル社