Comprehensive analysis of CCN family upon mice tooth morphogenesis

• Project/Area Number: 24792272
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Tohoku University
• Principal Investigator: HASEGAWA Masakazu 東北大学, 大学病院, 助教 (40625042)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: CCN / μCT / 再生医療 / CCNファミリー

Research Abstract

For analysis of CCN family effect upon mice tooth morphogenesis, we cultured murine tooth germ obtained from upper and lower jaw of embryonic day 11.5 and 13.5 mice in organ culture system contained 10% FBS, 100mg/ml ascorbic acid, and 2mM L-glutamine, supplemented with recombinant CCN1, 2, 3, 4, 5, and 6, respectively. For three dimensional analysis of these organ tissue, we preliminary acquired the CT data of tooth germ of wild type mice and evaluated volume, maximum tooth length and degree of ossification.

Research Products

• [Journal Article] Improvement of asymmetric stomatognathic functions, unilateral crossbite, and facial esthetics in a patient with skeletal Class III malocclusion and mandibular asymmetry, treated with orthognathic surgery. (2013)
  • Author(s): Takeshita N, Ishida M, Watanabe H, Hashimoto T, Daimaruya T, Hasegawa M, Takano-Yamamoto T.
  • Journal Title: American journal of orthodontics and dentofacial orthopedics Volume: 144 Issue: 3 Pages: 441-454
  • DOI: 10.1016/j.ajodo.2012.09.024
  • Peer Reviewed
• [Journal Article] Inflammatory and Necrotic Effects of Minodronate, a Nitrogen-Containing Bisphosphonate, in Mice (2013)
  • Author(s): Kiyama T, Okada S, Tanaka Y, Kim S, Bando K, Hasegawa M, Yamaguchi K, Takano-Yamamoto T, Sasaki K, Sugawara S, Endo Y.
  • Journal Title: The Tohoku Journal of Experimental Medicine Volume: 230 Issue: 3 Pages: 141-149
  • DOI: 10.1620/tjem.230.141
  • NAID: 10031189109
  • ISSN: 0040-8727, 1349-3329
  • Peer Reviewed
• [Journal Article] Orthodontic miniscrew failure rate and root proximity, insertion angle, bone contact length, and bone density. (2013)
  • Author(s): Watanabe H, Deguchi T, Hasegawa M, Ito M, Kim S, Takano-Yamamoto T
  • Journal Title: Orthod Craniofac Res Volume: 16(1) Issue: 1 Pages: 44-55
  • DOI: 10.1111/ocr.12003
  • Peer Reviewed
• [Presentation] Application of a Level-set Method for Simulation of Orthodontic Tooth Movement Using CT Image-based Voxel FE Models (2012)
  • Author(s): Taiji Adachi, Masakazu Hasegawa, Teruko Takano-Yamamoto
  • Organizer: 10th World Congress on Computational Mechanics
  • Place of Presentation: Sao Paulo, Brazil