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Genome, epigenome compaired analysis of a pair of monozygotic twins discordant for congenital malformation

Research Project

Project/Area Number 24792305
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthodontic/Pediatric dentistry
Research InstitutionShowa University

Principal Investigator

YOROZUYA REIKO  昭和大学, 歯学部, 助教 (30384340)

Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsエピジェネティクス / ゲノム / 一卵性双生児 / 口蓋裂 / 先天異常 / エピゲノム / 双生児 / 唇顎口蓋裂 / メチル化 / 環境要因
Outline of Final Research Achievements

Monozygotic (MZ) twins show remarkable resemblance in many aspects of disease. MZ twins were usually called “genetically identical”; however, evidence for genetic and epigenetic differences within rare MZ twins has accumulated. Nonsyndromic cleft lip and palate (NCLP) is one of the most common birth defects. It causes severe facial dysmorphism and treatment requires a multifaceted team approach. The causes of NCLP are multifactorial with both genetic and environmental factors. Some study shows that approximately 20% of the genetic contributions to NCLP have been assigned to a small number of genes.
Subjects are two pairs of NCLP/non-NCLP MZ and one pair of NCLP/non-NCLP triplets. We had used Methylated DNA Binding Domain (MBD) for inspissations of methylated DNA domain, and did MBD-based genom-wide methylation sequencing. We used EdgeR software to perform a differential methylation analysis.
Few specific high/low methylated gene were found in NCLP subjects.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • 2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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