Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Outline of Final Research Achievements |
Monozygotic (MZ) twins show remarkable resemblance in many aspects of disease. MZ twins were usually called “genetically identical”; however, evidence for genetic and epigenetic differences within rare MZ twins has accumulated. Nonsyndromic cleft lip and palate (NCLP) is one of the most common birth defects. It causes severe facial dysmorphism and treatment requires a multifaceted team approach. The causes of NCLP are multifactorial with both genetic and environmental factors. Some study shows that approximately 20% of the genetic contributions to NCLP have been assigned to a small number of genes. Subjects are two pairs of NCLP/non-NCLP MZ and one pair of NCLP/non-NCLP triplets. We had used Methylated DNA Binding Domain (MBD) for inspissations of methylated DNA domain, and did MBD-based genom-wide methylation sequencing. We used EdgeR software to perform a differential methylation analysis. Few specific high/low methylated gene were found in NCLP subjects.
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