Research of marker gene expression by epigenetic control in human periodontal ligament fibroblasts

• Project/Area Number: 24792316
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Tsurumi University
• Principal Investigator: ISHIKAWA Misao 鶴見大学, 歯学部, 助教 (90582445)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 歯根膜線維芽細胞 / アルカリフォスファターゼ / マイクロアレイ / RT-PCR法 / バイサルファイトシーケンス

Research Abstract

Alkaline phosphatase (ALP) gene is expressed strongly in human periodontal ligament fibroblasts. We examined the changes of the gene expression and activity by epigenetics control (by DNA methylation in particular) using a bisulfite sequence analysis of ALP promoter region.These results suggested that expression of ALP gene is not under the control of epigenetics, and the change of activity is affected by transcription factors.